Integrated cascade nanozyme catalyzes in vivo ROS scavenging for anti-inflammatory therapy

Abstract

Here, an integrated cascade nanozyme with a formulation of Pt@PCN222-Mn is developed to eliminate excessive reactive oxygen species (ROS). This nanozyme mimics superoxide dismutase by incorporation of a Mn–[5,10,15,20-tetrakis(4-carboxyphenyl)porphyrinato]–based metal-organic framework compound capable of transforming oxygen radicals to hydrogen peroxide. The second mimicked functionality is that of catalase by incorporation of Pt nanoparticles, which catalyze hydrogen peroxide disproportionation to water and oxygen. Both in vitro and in vivo experimental measurements reveal the synergistic ROS-scavenging capacity of such an integrated cascade nanozyme. Two forms of inflammatory bowel disease (IBD; i.e., ulcerative colitis and Crohn’s disease) can be effectively relieved by treatment with the cascade nanozyme. This study not only provides a new method for constructing enzyme-like cascade systems but also illustrates their efficient therapeutic promise in the treatment of in vivo IBDs.

Nanoplatforms for Sepsis Management: Rapid Detection/Warning, Pathogen Elimination and Restoring Immune Homeostasis

“…In contrast, inorganic nanozymes that eliminate excessive ROS are more desirable at management of inflammatory diseases such as inflammatory bowel diseases (IBDs) [ 71 ]. To eliminate ROS more efficiently at IBDs, Liu and coworkers established an integrated cascade nanozyme by introducing a superoxide dismutase (SOD)-like Mn (III) porphyrin and a catalase (CAT)-like Pt NP into a nanoscale Zr-based metal–organic frameworks (MOF), PCN222 [ 83 ]. The resultant cascade nanozyme named Pt@PCN222-Mn could transform the catalytic process of ·O 2 − to H 2 O/O 2 from inherent two transport steps to a high-performance cascade catalysis in single compartment.…”

Metal–Organic Frameworks Enhance Biomimetic Cascade Catalysis for Biosensing

“…This strategy has been confirmed to have gratifying potential in biocatalysis, biomedicine, and biosensing. [39][40][41][42][43][44][45] Herein, the research progress on the design and construction of MOFs-involving cascade reaction systems for signal amplification in biosensing is comprehensively summarized (Scheme 1). First, the MOFs-involving biomimetic cascade systems are mainly divided into three types based on the component of the cascade reaction catalyst, including enzymes, enzymes-nanozymes, and nanozymes.…”

“…This strategy has been confirmed to have gratifying potential in biocatalysis, biomedicine, and biosensing. [ 39–45 ]…”

Protective Effect of Platinum Nano-antioxidant and Nitric Oxide Against Hepatic Ischemia-Reperfusion Injury

“…Among these nanozymes, several nanomaterials have displayed promising effect on the elimination of reactive oxygen species (ROS), which could be employed as biomimetic antioxidants to regulate ROS homeostasis 25,27−30 . In addition, nanozymecatalyzed cascade reactions are becoming intriguing for versatile biomedical applications [31][32][33][34] . For example, Li et al integrated the arti cial Au nanoparticle (NP) nanozyme and natural ATP synthase into hollow silica microspheres for mitochondria-mimicking oxidative phosphorylation 31 .…”

“…In the designed natural-arti cial hybrid architecture, the Au NPs could convert glucose into gluconic acid in the presence of oxygen (O 2 ) and the resulting transmembrane proton gradient facilitated the production of ATP catalyzed by ATP synthase. Nanozyme-involved cascade reactions exhibit great bene ts in reducing the diffusion barriers, minimizing intermediate decomposition and enhancing local concentrations of reactants, thereby improving the intercommunication and e ciency of catalytic reactions 33,34 .…”