Integrated control of white rot disease on beans caused by Sclerotinia sclerotiorum using Contans® and reduced fungicides application

El-Sheshtawi, M.; Elkhaky, Maged T.; Sayed, Shaban R. M.; Bahkali, Ali H.; Mohammed, Arif A.; Gambhir, Dikshit; Mansour, Aref S.; Elgorban, Abdallah M.

doi:10.1016/j.sjbs.2016.01.038

Cited by 25 publications

(15 citation statements)

References 16 publications

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“…The treatment with the fungicide (Vitavax) showed the best results in reducing disease incidence and severity (6.67±1.18 and 0.88±0.08, respectively). Similar results were obtained by Elsheshtawi et al (2017) who reported that the "Topsin" fungicide significantly reduced the disease incidence caused by S. sclerotiorum by 90% and 95% survival plants, respectively.…”

Section: Pot Experimentssupporting

confidence: 88%

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The dual inoculation with Rhizobium sp. and cyanobacterial extracts enhances the common bean (Phaseolus vulgaris L.) responses to white rot disease caused by Sclerotinia sclerotiorum

2019

Middle East J. Appl. Sci.

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In this study, laboratory and greenhouse experiments were conducted to investigate the suppression effect of two biocontrol agents caynobacterial (Spirulina sp., Nostoc linckia and Anabaena variabilis) extracts, along with Rhizobium leguminosarum biovar phaseoli, against Sclerotinia sclerotiorum in common bean plants. Four isolates of S. sclerotiorum were tested for pathogenicity in bean plants, and all isolated fungi proved to be pathogenic and caused white rot symptoms. Results of in vitro studies showed that algal extracts significantly inhibited the mycelial growth of the pathogen when compared to the untreated control. N. linckia gave the highest reduction (56.29%), followed by A. variabilis (51.85%) and Spirulina sp. (45.93%), respectively compared to control (0%). In greenhouse experiments, the combined effect of Rhizobium sp. and cyanobacterial extracts significantly reduced disease incidence and severity under artificial infection with S. sclerotiorum. The treatments showed the maximum effects for controlling disease incidence and severity caused by S. sclerotiorum, which were in the range of 13.33 to 26.67 % and 1.24 to 1.82, compared to 73.33 and 4.50 % in infested control, respectively. In addition, these treatments increased number of nodules, plant height, root length, fresh and dry weight of shoots, N2 % and total nitrogen compared to control. The effects were similar to those of the fungicide Vitavax, which reduced the disease incidence and severity but adversely affected Rhizobium sp. and the symbiotic N2 fixing parameters. Considerable increases in activity of oxidative reductive enzymes (peroxidase and polyphenol oxidase) were recorded in plants grown from treated bean seeds.

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Section: Pot Experimentssupporting

confidence: 88%

“…Isolates were purified and identified according to their morphological and microscopical characteristics as Sclerotinia sclerotiorum. This fungus was previously reported to be associated with bean white rot diseases (Khalequzzaman and Hossain, 2008;Aysan andDemir, 2009 andElsheshtawi et al, 2017).…”

Section: Isolation and Identification Of The Causal Organismsmentioning

confidence: 87%

The dual inoculation with Rhizobium sp. and cyanobacterial extracts enhances the common bean (Phaseolus vulgaris L.) responses to white rot disease caused by Sclerotinia sclerotiorum

2019

Middle East J. Appl. Sci.

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“…Given this, the application of chemical fungicides, such as picoxystrobin (Aproach ® ) and boscalid (Endura ® ), is the standard procedure used for the control of SSR [26,27]. Fungal biological controllers, such as Trichoderma asperelloides, Trichoderma harzianum, and Coniothyrium minitans e.g., [28][29][30], have been considered to be a viable alternative for the treatment of soybean seed, although alterations in the water potential of the soil may limit the use of these fungi for biocontrol [31]. Even so, the ongoing advances in the development of new microbial strains with potential for biological control provide increasing hope for the discovery of new, as yet untested strains that are more effective for the suppression of Sclerotinia.…”

Section: Introductionmentioning

confidence: 99%

Biocontrol Potential of Sclerotinia sclerotiorum and Physiological Changes in Soybean in Response to Butia archeri Palm Rhizobacteria

Vitorino

Silva

Cruvinel

et al. 2020

Plants

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Sclerotinia sclerotiorum is a necrotrophic parasitic fungus that causes Sclerotinia stem rot (SSR), which is currently one of the most difficult agronomic crop diseases to control. A number of plants of the Brazilian Cerrado biome have been shown to be important sources of symbiotic microorganisms with biotechnological potential, so we decided to test the potential of bacteria isolated from the dwarf jelly palm, Butia archeri (Arecaceae) for the control of the pathogenic effects provoked by S. sclerotiorum. For this, we bioprimed seeds and evaluated the effects of this biopriming on the OJIP transient patterns prior to and following infection by the phytopathogen. Plants treated with the BA48R strain of Enterobacter sp., and in particular, those treated with the BA88R strain of Bacillus cereus presented the best results in terms of the loss/gain of the physiological and symptomatological variables evaluated. The plants bioprimed with BA88R presented high post-infection levels of total chlorophyll (33.35 FCIs) and chlorophyll a (26.39 FCIs), maintained a high Nitrogen Balance Index (NBI = 18.87), and synthesized low concentrations of flavonoids (1.39). These plants also maintained high levels of PIABS (1.111) and PITOTAL (1.300) following infection, and low levels of Di0/RC (0.602), which indicates that, in the presence S. sclerotiorum, the efficiency of the photosynthesis in the plants treated with these bacteria was less affected in the reaction centers, as confirmed by the negative amplitude recorded in the L band. The present study reconfirms the importance of the use of chlorophyll fluorescence for the diagnosis of disease and conditions of stress in crop plants, in addition to demonstrating the effectivenesss of the BA48R bacterial strain and, in particular, the BA88R strain on systemic resistance induction and suppression of S. sclerotiorum in Glycine max plants, with enormous potential for the development of more sustainable agricultural processes.

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“…and produce antifungal substances that inhibit host growth [5,[7][8][9][10][11][12][13]. C. minitans can successfully control crop diseases caused by S. sclerotiorum in the field [14][15][16]. C. minitans destroyed the sclerotia, blocked the germination of apothecia by approximately 90% and reduced disease incidence in a bean crop by 50% [5,17].…”

Section: Introductionmentioning

confidence: 99%

Mycoparasitism illuminated by genome sequencing and digital gene expression profiling of Coniothyrium minitans, an important biocontrol fungus of the plant pathogen Sclerotinia sclerotiorum

Zhao¹,

Zhou²,

Xiè³

et al. 2019

Preprint

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BackgroundConiothyrium minitans is a mycoparasite of the notorious plant pathogen Sclerotinia sclerotiorum. To further understand the parasitism of C. minitans, here, we assembled and analyzed its genome by combining transcriptome data. ResultsThe genome of C. minitans strain ZS-1 was 39.77 Mb in 350 scaffolds. A total of 11437 predicted genes and proteins were annotated, and 30.8% of blast hits matched proteins encoded by Paraphaeosphaeria sporulosa, a worldwide soil fungus. The transcriptome of strain ZS-1 during the early interaction at 0 h, 4 h and 12 h with its host was analyzed. The detected expressed genes were involved in response to host defenses, including cell wall-degrading enzymes, transporters, secretory proteins and secondary metabolites. The fungal cell wall-degrading enzymes belonged to the GH16, GH18, and GH72 classes in CAZymes, and some were significantly up-regulated during mycoparasitism. Most of the monocarboxylate transporter genes of the major facilitator superfamily and all the detected ABC transporters, especially the heavy metal transporters, were significantly upregulated. Approximately 8% of the 11437 proteins in C. minitans were predicted to be secretory proteins, with catalytic activity, hydrolase activity, peptidase activity and serine hydrolase activity enriched in molecular function. Most genes involved in serine hydrolase activity were significantly upregulated during mycoparasitism. ConclusionThis assemble genome and genome-wide expression study demonstrate that the mycoparasitism process of C. minitans is complex and a series of genes or proteins would be deployed by C. minitans to invade successfully the host. Our study provides insights into the mechanisms of the mycoparasitism between C. minitans and S. sclerotiorum and clues to excavate active secondary metabolites from C. minitans. BackgroundConiothyrium minitans, a sclerotial mycoparasite, lives on the sclerotia of Sclerotinia sclerotiorum and its relatives in soil and was first isolated and identified in 1947 by Campbell [1]. It is distributed worldwide in America, Europe, Australia, Africa and Asia [2][3][4], and its remarkable biocontrol ability has attracted great attention. C. minitans shares similar conditions (20°C) with its host S. sclerotiorum [1,[4][5][6]. C. minitans has a narrow host range, and most of its reported hosts are fungi in the genus Sclerotinia [5]. It can parasitize the sclerotia and hypha of Sclerotinia spp. and produce antifungal substances that inhibit host growth [5,[7][8][9][10][11][12][13]. C. minitans can successfully control crop diseases caused by S. sclerotiorum in the field [14][15][16]. C. minitans destroyed the sclerotia, blocked the germination of apothecia by approximately 90% and reduced disease incidence in a bean crop by 50% [5, 17]. As the main effective constituent in commercial biological control agents, C. minitans was first registered in Germany and was used to control the diseases caused by S. sclerotiorum and S. minor in Europe, America, Oceania and Asia. In China, C. min...

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Integrated control of white rot disease on beans caused by Sclerotinia sclerotiorum using Contans® and reduced fungicides application

Cited by 25 publications

References 16 publications

The dual inoculation with Rhizobium sp. and cyanobacterial extracts enhances the common bean (Phaseolus vulgaris L.) responses to white rot disease caused by Sclerotinia sclerotiorum

The dual inoculation with Rhizobium sp. and cyanobacterial extracts enhances the common bean (Phaseolus vulgaris L.) responses to white rot disease caused by Sclerotinia sclerotiorum

Biocontrol Potential of Sclerotinia sclerotiorum and Physiological Changes in Soybean in Response to Butia archeri Palm Rhizobacteria

Mycoparasitism illuminated by genome sequencing and digital gene expression profiling of Coniothyrium minitans, an important biocontrol fungus of the plant pathogen Sclerotinia sclerotiorum

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