Integrated Karyotypes of Diploid and Tetraploid Carrizo Citrange (Citrus sinensis L. Osbeck × Poncirus trifoliata L. Raf.) as Determined by Sequential Multicolor Fluorescence in situ Hybridization With Tandemly Repeated DNA Sequences

Deng, Honghong; Tang, Guohao; Xu, Nuo; Gao, Zhijian; Lin, Lijin; Liang, Dong; Qunxian, Deng; Wang, Jin; Cai, Zexi; Liang, Guolu; Lv, Xiulan

doi:10.3389/fpls.2020.00569

Cited by 9 publications

(5 citation statements)

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“…(AG 3 T 3 ) 3 , a classic chromosome end marker, is typically located in the distal region of the chromosome in H. mutabilis [ 32 ], J. regia , J. sigillata [ 31 ], F. pennsylvanica , S. oblata , L. lucidum , L. × vicaryi [ 28 ], B. diaphana , and B. soulieana [ 28 ]. Other similar types of (T x A y G z ) n [ 49 ] have also been identified at each chromosome end in the woody plants C. sinensis × P. trifoliata [ 23 ], Citrus clementina Hort. Ex Tan.…”

Section: Discussionmentioning

confidence: 99%

“…Occasionally, (AG 3 T 3 ) 3 or other similar types deviated from the end and were observed in the proximal and interstitial regions of chromosomes in the woody plant Ci. sinensis × P. trifoliata [ 23 ], Ch. campanulatus [ 30 ], Aralia elata (Miq.)…”

Section: Discussionmentioning

confidence: 99%

“…5S rDNA has been used extensively as a chromosome marker and exhibits substantial conservation and stability in woody plants Annona cherimola L. [ 61 ], C. sinensis × P. trifoliata [ 23 ], A. elata , D. morbiferus , E. sessiliflorus , K. septemlobus [ 51 ], Ch. campanulatus [ 30 ], G. biloba and P. densiflora [ 52 ], H. rhamnoides [ 17 ], R. wichurana [ 53 ], Passiflora species [ 62 ], Cestrum species [ 56 ], and V. foetida [ 57 ].…”

Section: Discussionmentioning

confidence: 99%

“…These oligonucleotide sequences can then be readily produced and tagged with fluorescent markers for use as oligo-probes in FISH [ 18 ]. Species identification based on such oligo-probes has been reported in an increasing number of plant species, such as Avena L. species [ 19 ], Arachis hypogaea L. [ 20 ], Saccharum spontaneum L. [ 21 ], Citrus L. species [ 22 ], Citrus sinensis (L.) Osbeck × Poncirus trifoliata (L.) Raf., CC [ 23 ], Populus L. species [ 24 ], Strobus Opiz species [ 25 ], and Pinus L. species [ 26 ]. However, information regarding H. rhamnoides is limited.…”

Section: Introductionmentioning

confidence: 99%

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Oligo-FISH Can Identify Chromosomes and Distinguish Hippophaë rhamnoides L. Taxa

Luo

Liu

2022

Genes

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Oligo-fluorescence in situ hybridization (FISH) facilitates precise chromosome identification and comparative cytogenetic analysis. Detection of autosomal chromosomes of Hippophaë rhamnoides has not been achieved using oligonucleotide sequences. Here, the chromosomes of five H. rhamnoides taxa in the mitotic metaphase and mitotic metaphase to anaphase were detected using the oligo-FISH probes (AG3T3)3, 5S rDNA, and (TTG)6. In total, 24 small chromosomes were clearly observed in the mitotic metaphase (0.89–3.03 μm), whereas 24–48 small chromosomes were observed in the mitotic metaphase to anaphase (0.94–3.10 μm). The signal number and intensity of (AG3T3)3, 5S rDNA, and (TTG)6 in the mitotic metaphase to anaphase chromosomes were nearly consistent with those in the mitotic metaphase chromosomes when the two split chromosomes were integrated as one unit. Of note, 14 chromosomes (there is a high chance that sex chromosomes are included) were exclusively identified by (AG3T3)3, 5S rDNA, and (TTG)6. The other 10 also showed a terminal signal with (AG3T3)3. Moreover, these oligo-probes were able to distinguish one wild H. rhamnoides taxon from four H. rhamnoides taxa. These chromosome identification and taxa differentiation data will help in elucidating visual and elaborate physical mapping and guide breeders’ utilization of wild resources of H. rhamnoides.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Oligo-FISH Can Identify Chromosomes and Distinguish Hippophaë rhamnoides L. Taxa

Luo

Liu

2022

Genes

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“…Typical chromosomal loci largely used as FISH probes are represented by tandem repetitive arrays of 5S and 45S rRNA genes and satellite DNA sequences whose chromosome location is species-specific (e.g., [25,[39][40][41][42]). Another extensively employed probe types are BAC (bacterial artificial chromosome) clones bearing large single-and low-copy DNA sequences [43,44] or certain mobile repetitive elements dispersed on all chromosomes but provide signals on specific chromosomal regions, e.g., in centromeres [8,[45][46][47].…”

Section: Molecular Cytogenetics Of Musaceaementioning

confidence: 99%

Advances in the Molecular Cytogenetics of Bananas, Family Musaceae

Šimoníková

Čížková

Zoulová

et al. 2022

Plants

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The banana is a staple food crop and represents an important trade commodity for millions of people living in tropical and subtropical countries. The most important edible banana clones originated from natural crosses between diploid Musa balbisiana and various subspecies of M. acuminata. It is worth mentioning that evolution and speciation in the Musaceae family were accompanied by large-scale chromosome structural changes, indicating possible reasons for lower fertility or complete sterility of these vegetatively propagated clones. Chromosomal changes, often accompanied by changes in genome size, are one of the driving forces underlying speciation in plants. They can clarify the genomic constitution of edible bananas and shed light on their origin and on diversification processes in members of the Musaceae family. This article reviews the development of molecular cytogenetic approaches, ranging from classical fluorescence in situ hybridization (FISH) using common cytogenetic markers to oligo painting FISH. We discuss differences in genome size and chromosome number across the Musaceae family in addition to the development of new chromosome-specific cytogenetic probes and their use in genome structure and comparative karyotype analysis. The impact of these methodological advances on our knowledge of Musa genome evolution at the chromosomal level is demonstrated. In addition to citing published results, we include our own new unpublished results and outline future applications of molecular cytogenetics in banana research.

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Heterochromatic patterns of Citrus revisited: a new look at species origins and karyotype evolution

Montenegro

Roa

Filho

et al. 2023

Tree Genetics & Genomes

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Integrated Karyotypes of Diploid and Tetraploid Carrizo Citrange (Citrus sinensis L. Osbeck × Poncirus trifoliata L. Raf.) as Determined by Sequential Multicolor Fluorescence in situ Hybridization With Tandemly Repeated DNA Sequences

Cited by 9 publications

References 44 publications

Oligo-FISH Can Identify Chromosomes and Distinguish Hippophaë rhamnoides L. Taxa

Oligo-FISH Can Identify Chromosomes and Distinguish Hippophaë rhamnoides L. Taxa

Advances in the Molecular Cytogenetics of Bananas, Family Musaceae

Heterochromatic patterns of Citrus revisited: a new look at species origins and karyotype evolution

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