Integrated Karyotyping of Woodland Strawberry (&lt;b&gt;&lt;i&gt;Fragaria vesca&lt;/i&gt;&lt;/b&gt;) with Oligopaint FISH Probes

Qu, Manman; Li, Kunpeng; Han, Yan-Li; Chen, Lei; Li, Zongyun; Han, Yonghua

doi:10.1159/000485283

Cited by 45 publications

(31 citation statements)

References 24 publications

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“…Chromosome painting allows the identification and tracking of the target chromosome at different stages of mitosis and meiosis (Han et al, 2015). Oligo-based chromosome painting probes have been developed in an increasing number of plant species (Han et al, 2015;Qu et al, 2017;Braz et al, 2018;He et al, 2018;Hou et al, 2018;Meng et al, 2018;Albert et al, 2019), and have been used in various cytogenetic and genome studies (Jiang, 2019).…”

Section: Introductionmentioning

confidence: 99%

An extraordinarily stable karyotype of the woody Populus species revealed by chromosome painting

Xin

Zhang

et al. 2019

The Plant Journal

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Summary The karyotype represents the basic genetic make‐up of a eukaryotic species. Comparative cytogenetic analysis of related species based on individually identified chromosomes has been conducted in only a few plant groups and not yet in woody plants. We have developed a complete set of 19 chromosome painting probes based on the reference genome of the model woody plant Populus trichocarpa. Using sequential fluorescence in situ hybridization we were able to identify all poplar chromosomes in the same metaphase cells, which led to the development of poplar karyotypes based on individually identified chromosomes. We demonstrate that five Populus species, belonging to five different sections within Populus, have maintained a remarkably conserved karyotype. No inter‐chromosomal structural rearrangements were observed on any of the 19 chromosomes among the five species. Thus, the chromosomal synteny in Populus has been remarkably maintained after nearly 14 million years of divergence. We propose that the karyotypes of woody species are more stable than those of herbaceous plants since it may take a longer period of time for woody plants to fix chromosome number or structural variants in natural populations.

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Section: Introductionmentioning

confidence: 99%

An extraordinarily stable karyotype of the woody Populus species revealed by chromosome painting

Xin

Zhang

et al. 2019

The Plant Journal

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“…Lysák et al, 2001, Idziak et al, 2014. The development of oligo painting FISH (Han et al, 2015) changed this situation dramatically and it is now possible to label individual plant chromosomes and chromosomal regions in many species (Qu et al, 2017, Machado et al, 2018, Albert et al, 2019, Bi et al, 2020. (Fauré et al, 1993, Hippolyte et al, 2010, Mbanjo et al, 2012, Noumbissie et al, 2016.…”

Section: Discussionmentioning

confidence: 99%

Chromosome painting in cultivated banana and their wild relatives (Musaspp.) reveals differences in chromosome structure

Šimoníková

Němečková

Čížková

et al. 2020

Preprint

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Edible banana cultivars are diploid, triploid or tetraploid hybrids which originated by natural cross hybridization between subspecies of diploid Musa acuminata, or between M. acuminata and diploid M. balbisiana. Participation of two wild diploid species M. schizocarpa and M. textilis was also indicated by molecular studies. Fusion of gametes with structurally different chromosome sets may give rise to progenies with structural chromosome heterozygosity and reduced fertility due to aberrant chromosome pairing and unbalanced chromosome segregation. Only a few translocations have been classified on the genomic level so far and a comprehensive molecular cytogenetic characterization of cultivars and species of the family Musaceae is still lacking. FISH with chromosome-arm specific oligo painting probes was used for comparative karyotype analysis in a set of wild Musa species and edible banana clones. The results revealed large differences in chromosome structure discriminating individual accessions and permitted identification of putative progenitors of cultivated clones.

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“…If the genomic sequence is available from a target species, oligos, typically 40-50 bp long, specific to a chromosomal region or to an entire chromosome can be synthesized in parallel and labeled as a FISH probe (Han et al 2015). Oligo-FISH probes have been successfully developed in several plant species for various types of cytogenetic studies (Albert et al 2019;Bi et al 2020;He et al 2018;Hou et al 2018;Martins et al 2019;Meng et al 2018;Qu et al 2017;Simonikova et al 2019;Song et al 2020;Xin et al 2018;Xin et al 2020).…”

Section: Introductionmentioning

confidence: 99%

A universal chromosome identification system for maize and wildZeaspecies

Braz

Martins

Zhang

et al. 2020

Preprint

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Maize was one of the first eukaryotic species in which individual chromosomes can be identified cytologically, which made maize one of the oldest models for genetics and cytogenetics research. Nevertheless, consistent identification of all 10 chromosomes from different maize lines as well as from wild Zea species remains a challenge. We developed a new technique for maize chromosome identification based on fluorescence in situ hybridization (FISH). We developed two oligonucleotide-based probes that hybridize to 24 chromosomal regions. Individual maize chromosomes show distinct FISH signal patterns, which allow universal identification of all chromosomes from different Zea species. We developed karyotypes from three Zea mays subspecies and two additional wild Zea species based on individually identified chromosomes. A paracentric inversion was discovered on the long arm of chromosome 4 in Z. nicaraguensis and Z. luxurians based on modifications of the FISH signal patterns. Chromosomes from these two species also showed distinct distribution patterns of terminal knobs compared to other Zea species. These results support that Z. nicaraguensis and Z. luxurians are closely related species. ReferencesAdawy SSM, Stupar RM, Jiang J (2004) Fluorescence in situ hybridization of knob-associated DNA elements analysis reveals multiple loci in one-knob and knobless maize lines. J (2019) Whole-chromosome paints in maize reveal rearrangements, nuclear domains, and chromosomal relationships. P Natl Acad Sci USA 116:1679-1685 Amarillo FIE, Bass HW (2007) A transgenomic cytogenetic sorghum (Sorghum propinquum) bacterial artificial chromosome fluorescence in situ hybridization map of maize (Zea mays L.) pachytene chromosome 9, evidence for regions of genome hyperexpansion.

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Integrated Karyotyping of Woodland Strawberry (<b><i>Fragaria vesca</i></b>) with Oligopaint FISH Probes

Cited by 45 publications

References 24 publications

An extraordinarily stable karyotype of the woody Populus species revealed by chromosome painting

An extraordinarily stable karyotype of the woody Populus species revealed by chromosome painting

Chromosome painting in cultivated banana and their wild relatives (Musaspp.) reveals differences in chromosome structure

A universal chromosome identification system for maize and wildZeaspecies

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