Integrated metabolomics and network pharmacology approach to reveal immunomodulatory mechanisms of Yupingfeng granules

Li, Moying; Gao, Yong-jian; Yue, Xinyi; Zhang, Bei; Zhou, Haifeng; Yuan, Chunping; Wu, Tong

doi:10.1016/j.jpba.2020.113660

Cited by 13 publications

(13 citation statements)

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“…Performing the enrichment analyses of common targets as well as pneumonia targets, respectively, and selecting their overlapping terms, we found that these genes are highly involved in host immune-inflammatory responses, inhibiting cell migration. Li et al [ 18 ] confirmed that YPFG might play an immunomodulatory role by modulating lipid metabolism, thereby affecting bile acid metabolism and inflammatory response. The results of this literature are in agreement with our prediction.…”

Section: Discussionmentioning

confidence: 99%

“…YPFG is commonly used to treat inflammation-related diseases, including asthma [ 15 ], chronic obstructive pulmonary disease [ 16 ], and pneumonia [ 17 ]. Through metabolomics and network pharmacology approaches, Li et al [ 18 ] revealed that the immune-modulatory effects of YPFG may be mediated via lipid metabolism regulation. Liu et al [ 19 ] identified that YPFG suppresses NLRP3 inflammasome constituents to ameliorate asthma-associated inflammatory responses.…”

Section: Introductionmentioning

confidence: 99%

“…rough metabolomics and network pharmacology approaches, Li et al [18] revealed that the immune-modulatory effects of YPFG may be mediated via lipid metabolism regulation. Liu et al [19] identified that YPFG suppresses NLRP3 inflammasome constituents to ameliorate asthma-associated inflammatory responses.…”

Section: Introductionmentioning

confidence: 99%

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Deciphering the Mechanism of YuPingFeng Granules in Treating Pneumonia: A Network Pharmacology and Molecular Docking Study

Bi-rong

Luo

Liu

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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Objective. YuPingFeng Granules (YPFGs) is an herbal formula clinically used in China for more than 100 years to treat pneumonia. Nevertheless, the mechanism of YPFG in pneumonia treatment has not been established. This network pharmacology-based strategy has been performed to elucidate active compounds as well as mechanisms of YPFG in pneumonia treatment. Methods. First, active compounds of YPFG were identified in the traditional Chinese medicine systems pharmacology (TCMSP) database, and then the targets related to the active compounds were obtained from TCMSP and Swiss Target Prediction databases. Next, using DisGeNET, DrugBank, and GeneCards databases, we got therapeutic targets of pneumonia and common targets between pneumonia targets and YPFG. After that, a protein-protein interaction (PPI) network of pneumonia composed of common targets was built to analyze the interactions among these targets, which focused on screening for hub targets by topology. Then, online software and the ClusterProfiler package were utilized for the enrichment analysis of gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) data. Finally, the visualization software of Autodock was used for molecular docking among the hub target proteins. Results. 10 hub genes were selected by comparing the GO and KEGG functions of pneumonia targets with those of the common targets of YPFG and pneumonia. By using molecular docking technology, a total of 3 active ingredients have been verified as being able to combine closely with 6 hub targets and contribute to their therapeutic effects. Conclusion. This research explored the multigene pharmacological mechanism of action of YPFG against pneumonia through network pharmacology. The findings present new ideas for studying the mechanism of action of Chinese medicine against pneumonia caused by bacteria.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Deciphering the Mechanism of YuPingFeng Granules in Treating Pneumonia: A Network Pharmacology and Molecular Docking Study

Bi-rong

Luo

Liu

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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“…Network pharmacology is based on the interaction between “disease-gene-target-drug,” and constructs the related network from the perspective of biological system, explores the pathogenesis and course development of diseases, and reveals the interaction between various targets of drugs in the body, which is beneficial to the multi-level exploration of the action mechanism of active compounds of traditional Chinese medicine ( Bing et al, 2017 ). The research methods of metabonomics and network pharmacology based on the holistic view are consistent with the systematic view advocated by traditional Chinese medicine research, so they are especially suitable for studying the action mechanism of complex systems of traditional Chinese medicine ( Li et al, 2021 ).…”

Section: Introductionmentioning

confidence: 89%

Integrated Metabolomics and Network Pharmacology Analysis Immunomodulatory Mechanisms of Qifenggubiao Granules

Guo¹,

Dong²,

Huo³

et al. 2022

Front. Pharmacol.

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Background: Qifenggubiao granules (QFGBG) is a new Chinese medicine independently developed by Heilongjiang Academy of Traditional Chinese Medicine, which combines the essence of Yupingfeng powder and Shengmai yin (invention patent number: CN1325098C, approval number: Sinopharm Zhunzi B20020410), and has been included in the 2020 edition of Chinese Pharmacopoeia. It has remarkable pharmacodynamic results and conclusive clinical effects in the treatment of allergic rhinitis, chronic cough and other diseases. Previous pharmacological studies have shown that it has immunomodulatory effect, but its immunomodulatory mechanism is still unclear.Methods: In this study, cyclophosphamide (CTX) was used to establish the immune hypofunction model in mice, and the weight change, index of immune organs in spleen and thymus, pathological sections of immune organs and inflammatory factors were used to evaluate the model. Based on the metabolic biomarkers obtained by metabonomics technology, the potential targets of Qifeng Gubiao Granule immunomodulation were obtained by integrating the targets of blood components, metabolites and diseases through network pharmacology. Meanwhile, GO enrichment analysis and KEGG pathway analysis were carried out on the potential targets.Results: QFGBG can increase body weight and organ index, and recover immune organ damage caused by CP. Metabonomics identified 13 metabolites with significant changes, among which the level of phospholipid (PC) metabolites decreased significantly in the model group. Sphingosine -1- phosphate, 1- palmitoyl phosphatidylcholine [LysoPC (16:0/0:0)] and other metabolites were significantly increased in the model group, and 98 targets of Qifeng’s external immune regulation were obtained by intersecting 629 component targets, 202 metabolite targets and 1916 disease targets. KEGG pathway analysis obtained 233 related metabolic pathways, and the top 20 metabolic pathways mainly involved IL-17 signaling pathway, TNF signaling pathway, Sphingolipid signaling pathway, and so on.Conclusion: QFGBG may act on AKT1, IL6, MAPK3, PTGS2, CASP3, MAPK1, ESR1, PPARG, HSP90AA1, PPARA and other targets, acting through Sphingolipid signaling pathway and signaling pathway. Combined with pharmacodynamic evaluation, the immunomodulatory effect of QFGBG was confirmed, and the immunomodulatory mechanism of QFGBG with multiple targets and multiple pathways was preliminarily clarified.

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“…It has been used for centuries in TCM and is currently used for treatment of asthma, bronchitis, allergic dermatitis and urticaria. Animal and in vitro studies have reported immunomodulator properties including reduction in tumor necrosis factor alpha, interleukin‐6, mucus secretion and modification of factors such as MUC5A which are involved in producing inflammatory cells and cytokines, T‐cell differentiation, and serum levels of IgA, IgG, IgM, and CD3 + T cells 9 …”

mentioning

confidence: 99%

Lost in translation: Evaluating traditional Chinese medicine by western standards

Leon

Allen

2022

Pediatric Investigation

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Integrated metabolomics and network pharmacology approach to reveal immunomodulatory mechanisms of Yupingfeng granules

Cited by 13 publications

References 2 publications

Deciphering the Mechanism of YuPingFeng Granules in Treating Pneumonia: A Network Pharmacology and Molecular Docking Study

Deciphering the Mechanism of YuPingFeng Granules in Treating Pneumonia: A Network Pharmacology and Molecular Docking Study

Integrated Metabolomics and Network Pharmacology Analysis Immunomodulatory Mechanisms of Qifenggubiao Granules

Lost in translation: Evaluating traditional Chinese medicine by western standards

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