Integrated metabolomics and transcriptomics analysis during seed germination of waxy corn under low temperature stress

Abstract

Background Waxy corn has a short growth cycle and high multiple cropping index. However, after being planted in early spring, late autumn and winter, it is susceptible to low temperature (LT), which reduces the emergence rate and yield. Therefore, it is important to analyze the response mechanism of waxy corn under LT stress. Results All phenotype indexes of waxy corn inbred lines N28 were significantly higher than waxy corn inbred lines N67 under LT. With the increase of LT stress time, all physiological indexes showed an upward trend in N28 and N67. Differentially expressed genes (DEGs) 16,017 and 14,435 were identified in N28 and N67 compared with nongerminated control under LT germination, respectively, and differential metabolites 127 and 93 were detected in N28 and N67, respectively. In addition, the expression level of some genes involved in plant hormones and mitogen activated protein kinase (MAPK) signaling pathways was significantly up-regulated in N28. Compared with N67, flavonoid metabolites were also significantly enriched in N28 under LT germination. Conclusion Under LT stress, the inbred lines N28 was significantly higher than the inbred lines N67 in the phenotypic and physiological indices of cold resistance. Compared with N67, the expression levels of some genes involved in the plant hormones and MAPK pathways were significantly up-regulated in N28, and flavonoid metabolites were also significantly enriched in N28 under LT stress. These genes and metabolites may help N28 to improve cold resistance and may be as potential target genes for cold resistance breeding in waxy corn.

Integrating WGCNA and PPI network to screen heat stress-responsive hub genes of Pinellia ternata

“…The homogenate was subjected to centrifugation at 10,000 rpm for 15 min at 4℃. The supernatant was used for the determination of SOD and POD activities, and the method of determination was referred to in the literature [34,35] .…”

Multiomics Analyses Reveal the Dual Role of Flavonoids in Pigmentation and Abiotic Stress Tolerance of Soybean Seeds

“…12 The higher level of flavonoids probably contribute to the cold tolerance in inbred line N28 waxy corn. 13 Overexpression of MdmiR156n promoted the accumulation of flavonoids and reduced the accumulation of ROS under drought conditions in transgenic apple calli and Arabidopsis thaliana plants. 14 The ectopic expression of flavonoids synthetase genes from Apocynum venetum including AvF3H, AvF3′H, and AvFLS notably increased the salt tolerance but reduced the ROS content in transgenic Arabidopsis, suggesting that the flavonoids improve the salt tolerance by increasing the ROS scavenging capacity in plants.…”

“…The cold responsive module BcCBF2–BcMYB111–BcF3H/BcFLS1 in Brassica campestris L. activates the biosynthesis of flavonoids, especially including the quercetin and kaempferol, to combat the cold stress . The higher level of flavonoids probably contribute to the cold tolerance in inbred line N28 waxy corn . Overexpression of MdmiR156n promoted the accumulation of flavonoids and reduced the accumulation of ROS under drought conditions in transgenic apple calli and Arabidopsis thaliana plants .…”

Integrated transcriptomic and metabolomic data reveal the cold stress responses molecular mechanisms of two coconut varieties

“…Integrated transcriptomics and metabolomics approaches are gaining prevalence to uncover molecular dynamics of abiotic stress resilience in various crops owing to genetical, morphological, and physiological data ( Maruyama et al., 2014 ; Jiang et al., 2023 ; Li Q. et al, 2023 ). In spite of this, there have been limited studies evaluating coconuts under cold stress by analyzing metabolomics and transcriptomics data.…”