Integrated method of thermosensitive triblock copolymer–salt aqueous two phase extraction and dialysis membrane separation for purification of lycium barbarum polysaccharide

Wang, Yun; Hu, Xiaowei; Han, Juan; Ni, Liang; Xu, Tao; Hu, Yunxiang; Chen, Tong

doi:10.1016/j.foodchem.2015.08.016

Cited by 46 publications

(19 citation statements)

References 31 publications

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“…As intelligent materials, the studies about block copolymer that contains thermo‐sensitive segment have been extensively reported naturally. Because the thermo‐sensitive block copolymer can form a hydrophobic core‐hydrophilic shell through self‐assembly in a selective solvent, it has displayed a prospect application potential in the range of medicine, nanomaterials engineering, and tissue engineering . Meanwhile, the research on poly (N‐isopropylacrylamide) (PNIPAM) block copolymer is more systematic and in‐depth .…”

Section: Introductionmentioning

confidence: 99%

Design and synthesis study of the thermo‐sensitive copolymer carrier of penicillin G acylase

Chen

Liu

et al. 2018

Polymers for Advanced Techs

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Reversible‐addition‐fragmentation chain transfer polymerization method was adopted here in this study to prepare a series of thermo‐sensitive tri‐block copolymer carrier, poly(N,N‐diethylacrylamide‐b‐β‐hydroxyethyl methacrylate‐b‐glycidyl methacrylate), PDEA‐b‐PHEMA‐b‐PGMA (named as DHG). Their structures and temperature sensitivity performances were further characterized by the nuclear magnetic resonance, the gel permeation chromatography, and the fluorescence spectroscopy, respectively. It has been identified that the synthesis reaction of tri‐block copolymer was living polymerization. The thermo‐sensitivity study suggested that 2 monomers, β‐hydroxyethyl methacrylate and glycidyl methacrylate, played key roles on the lower critical solution temperature performance. Finally, above block copolymer was conscripted to immobilize penicillin G acylase; relationships among the number‐average molar weight ()trueM¯n of the 2 monomer, β‐hydroxyethyl methacrylate and glycidyl methacrylate, loading (L), and activity recover rate (Ar) of immobilized PGA were investigated. And the results showed that L and Ar arrived at 19 502 U and 92%, respectively.

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Section: Introductionmentioning

confidence: 99%

Design and synthesis study of the thermo‐sensitive copolymer carrier of penicillin G acylase

Chen

Liu

et al. 2018

Polymers for Advanced Techs

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“…The effect of system temperature (0–30°C) on the extraction of anthocyanin and VLP were studied. As a tri‐block copolymer L44(EOPOEO)‐phosphate ATPS (Haraguchi et al, ; Wang et al, ), the temperature has a significant effect on the phase diagrams of ATPS. Since the hydrogen bonding between hydrophilic block PEO and water molecules would be destroyed with raising temperature (Zhou & Chu, ), the dehydration process occurred on the mantle of micelles, which will affect the distribution of water molecules in the two phases.…”

Section: Resultsmentioning

confidence: 99%

“…The effect of system temperature (0-308C) on the extraction of anthocyanin and VLP were studied. As a tri-block copolymer L44(EOPOEO)phosphate ATPS (Haraguchi et al, 2004;Wang et al, 2016), the temperature has a significant effect on the phase diagrams of ATPS.…”

Section: Effect Of Temperaturementioning

confidence: 99%

“…Its principle is based on the selective distribution between two aqueous phases, which is similar to traditional organic solvent extraction. This technology is widely applied in separation and purification of bioactive substances, succeeding in the separation and purification of protein (Ding et al, ), enzymes (Aumiller Jr. et al, ), nucleic acid (Matos, Johansson, Queiroz, & Bulow, ), antibiotics (Yang et al, ), polysa9ccharide (Wang et al, ) and plant active components (Han et al, ; Liu et al, ) etc. The early aqueous two‐phase system focused on the polymer–polymer ATPS, such as the commonly used polyethylene glycol–dextran ATPS (Albertsson, ), which has the characteristics of high water content (80–90%) in system, small interfacial tension, good biocompatibility, simple operation, easy amplification and continuous operation.…”

Section: Introductionmentioning

confidence: 99%

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Separation, purification of anthocyanin and vitis linn polysaccharide from grape juice by the two-step extraction and dialysis

Wang

Han

et al. 2017

J Food Process Preserv

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Liquid liquid extraction, as a clean and green process, has a great potential in technology and innovation, which has been widely used in industrial production. In this paper, a liquid-liquid extraction process based on L44-NaH 2 PO 4 ATPS was studied for separation and purification of anthocyanin and vitis linn polysaccharide from the grape juice. The concentrations of L44 and NaH 2 PO 4 , temperature and pH as factors of effect on the extraction rates were discussed. Under the optimal conditions, the extraction rates of anthocyanin and vitis linn polysaccharides were 99.8% and 90.3%, respectively. The work subject is extraction of two compounds from grape juice, which can be very important for food, pharmaceutical and cosmetic industries. The vitis linn polysaccharides were further purified by dialysis and the impurity protein was successfully removed based on organic solvent precipitation method. Besides, the recycle of L44 was also achieved through secondary extraction. Practical applicationsA liquid-liquid extraction process based on L44-NaH 2 PO 4 ATPS was studied for separation and purification of anthocyanin and vitis linn polysaccharide from the grape juice. The efficient separation of two kinds of water-soluble components is of great significance in industrial production. It is very interesting for scientific communities and shows a great biotechnological potential of the ATPS purification process. The work subject is extraction of two compounds from grape juice, which can be very important for food, pharmaceutical and cosmetic industries.

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“…Thermosensitive polymers with inverse temperature solubility have attracted much of attention in the last decades, because they undergo a sharp change in their properties in response to a small change in temperature at their LCST (lower critical solution temperature). This behaviour has becoming interesting for a number of applications, such as controlled drug delivery systems, molecular separation, tissue engineering, water treatment and oil recovery …”

Section: Introductionmentioning

confidence: 99%

Carboxymethylcellulose Grafted to Amino‐Terminated Poly(N‐isopropylacrylamide): Preparation, Characterization and Evaluation of the Thermoassociative Behaviour at Low Concentrations

Marques

Lima

Balaban

2016

Macromolecular Symposia

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Amino-terminated poly(N-isopropylacrylamide) (PNIPAM-NH 2 ) was synthesized by free-radical polymerization in water by using the redox pair potassium persulfate/2-aminoethanethiol hydrochloride (KPS/AET.HCl) in a glove box under N 2(g) atmosphere. PNIPAM-NH 2 was then used to produce graft copolymers with carboxymethylcellulose (CMC) in water by applying a water-soluble carbodiimide and N-hydroxysuccinimide as activators. The samples were characterized by infrared spectroscopy and the thermal behaviour on solid state was investigated by thermogravimetry. Also, the rheological behaviour of the copolymers at 25 and 60 C was evaluated in water at 3 and 7 g/L. Contrary to CMC and PNIPAM-NH 2 , which had only one DTG peak, the thermal decomposition of the graft copolymers occurred at two steps, being the first DTG peak higher and second one lower than the corresponding precursors. Thermoassociative behaviour in water for CMC-g-PNIPAM graft copolymers depend on the amount of chain transfer agent used for the PNIPAM-NH 2 synthesis. Thermothickening effect occurred for the copolymer that had PNIPAM-NH 2 prepared with lower AET.HCl content, at a polymer concentration of 7 g/L, which is much lower than the concentrations previously reported in literature for graft copolymers with poly(N-isopropylacrylamide).

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Integrated method of thermosensitive triblock copolymer–salt aqueous two phase extraction and dialysis membrane separation for purification of lycium barbarum polysaccharide

Cited by 46 publications

References 31 publications

Design and synthesis study of the thermo‐sensitive copolymer carrier of penicillin G acylase

Design and synthesis study of the thermo‐sensitive copolymer carrier of penicillin G acylase

Separation, purification of anthocyanin and vitis linn polysaccharide from grape juice by the two-step extraction and dialysis

Carboxymethylcellulose Grafted to Amino‐Terminated Poly(N‐isopropylacrylamide): Preparation, Characterization and Evaluation of the Thermoassociative Behaviour at Low Concentrations

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