Integrated microfluidic probe station

Perrault, Cécile M.; Qasaimeh, Mohammad A.; Brastaviceanu, Tiberius; Anderson, Kari B.; Kabakibo, Y.; Juncker, David

doi:10.1063/1.3497302

Cited by 21 publications

(21 citation statements)

References 20 publications

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“…Furthermore, the MQ and floating gradients could be used to study large samples which are hard to cultivate inside closed channels, such as embryos or tissue slices 42 . Finally, it may be useful for surface patterning by positioning and scanning the MFP atop surfaces 9,43,44 .…”

Section: Discussionmentioning

confidence: 99%

Microfluidic quadrupole and floating concentration gradient

2011

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The concept of fluidic multipoles, in analogy to electrostatics, has long been known as a particular class of solutions of the Navier-Stokes equation in potential flows, however, experimental observations of fluidic multipoles and of their characteristics have not been reported yet. Here we present a two-dimensional microfluidic quadrupole and a theoretical analysis consistent with the experimental observations. The microfluidic quadrupole was formed by simultaneously injecting and aspirating fluids from two pairs of opposing apertures in a narrow gap formed between a microfluidic probe and a substrate. A stagnation point was formed at the center of the microfluidic quadrupole, and its position could be rapidly adjusted hydrodynamically. Following the injection of a solute through one of the poles, a stationary, tunable, and movable – i.e. “floating” – concentration gradient was formed at the stagnation point. Our results lay the foundation for future combined experimental and theoretical exploration of microfluidic planar multipoles including convective-diffusive phenomena.

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Section: Discussionmentioning

confidence: 99%

Microfluidic quadrupole and floating concentration gradient

2011

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“…The horizontal alignment of the sample can be set via three screws on the outer edges of the sample holder. 1,18 The head holder is mounted on a shorter motorized stage (Zaber Technologies Inc., T-LSM25A), having a stroke of 12.5 mm in the Z direction. The integration of X, Y and Z stages make the cMFP independent from external motorized stages (e.g.…”

Section: B Positioning Systemmentioning

confidence: 99%

A compact and versatile microfluidic probe for local processing of tissue sections and biological specimens

Cors

Lovchik

Delamarche

et al. 2014

Review of Scientific Instruments

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The microfluidic probe (MFP) is a non-contact, scanning microfluidic technology for local (bio)chemical processing of surfaces based on hydrodynamically confining nanoliter volumes of liquids over tens of micrometers. We present here a compact MFP (cMFP) that can be used on a standard inverted microscope and assist in the local processing of tissue sections and biological specimens. The cMFP has a footprint of 175 × 100 × 140 mm 3 and can scan an area of 45 × 45 mm 2 on a surface with an accuracy of ±15 µm. The cMFP is compatible with standard surfaces used in life science laboratories such as microscope slides and Petri dishes. For ease of use, we developed self-aligned mounted MFP heads with standardized "chip-to-world" and "chip-toplatform" interfaces. Switching the processing liquid in the flow confinement is performed within 90 seconds using a selector valve with a dead-volume of approximately 5 µL. We further implemented height-compensation that allows a cMFP head to follow non-planar surfaces common in tissue and cellular ensembles. This was shown by patterning different macroscopic copper-coated topographies with height differences up to 750 µm. To illustrate the applicability to tissue processing, 5 µm thick M000921 BRAF V600E+ melanoma cell blocks were stained with hematoxylin to create contours, lines, spots, gradients of the chemicals, and multiple spots over larger areas. The local staining was performed in an interactive manner using a joystick and a scripting module. The compactness, user-friendliness and functionality of the cMFP will enable it to be adapted as a standard tool in research, development and diagnostic laboratories, particularly for the interaction with tissues and cells.3

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“…Furthermore, some regions within the network cannot easily or selectively be accessed as in open-surface setups. 31,32 Indeed, these issues become even more critical when aiming to enclose large biological samples inside the chip, which limits the use of these devices with samples such as embryos or tissue slices. Beyond the issues of closed microfluidic systems, the switch from performing experiments using the traditional cell culture protocols (i.e.…”

Section: Introductionmentioning

confidence: 99%