Integrated microRNA/mRNA expression profiling of the skin of psoriasis patients

Delić, Denis; Wolk, Kerstin; Schmid, RM; Gabrielyan, Ogsen; Christou, Demetrios; Rieber, Kathrin; Rolser, Marcel; Jakob, Ines; Wiech, Franziska; Griesser, Manuela; Wohnhaas, Christian T.; Kokolakis, Georgios; Witte-Händel, Ellen; Baum, Patrick; Sabat, Robert

doi:10.1016/j.jdermsci.2019.11.003

Cited by 33 publications

(37 citation statements)

References 62 publications

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“…Another study demonstrated that miR-876-5p was downregulated in psoriasic tissue and blood of patients and would be expected to be biomarker and potential therapeutic targets for the treatment of psoriasis (22). In this study, the serum expression level of miR-106a-5p in psoriasis patients was higher than that in healthy control, which was consistent with two previous studies (5,11). We further studied the correlation between miR-106a-5p and PASI score.…”

Section: Discussionsupporting

confidence: 91%

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Diagnostic value of miR-106a-5p in patients with psoriasis and its regulatory role in inflammatory responses

Miao

Tong

et al. 2020

Preprint

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Background: Psoriasis is a multifactorial, recurring, and chronic inflammatory skin disease. This study was designed to explore the potential role of microRNA-106a-5p (miR-106a-5p) in psoriasis.Methods: The expression levels of miR-106a-5p in the serum of psoriasis patients and healthy individuals were detected by quantitative real-time polymerase chain reaction (qRT-PCR). The diagnostic value of miR-106a-5p in serum was evaluated by the receiver operating characteristics curve (ROC). The levels of interleukin-22 (IL-22), interleukin-17A (IL-17A), and tumor necrosis factor-alpha (TNF-alpha) were determined by enzyme-linked immunosorbent assay.Results: The serum expression of miR-106a-5p was found to be up-regulated in psoriasis patients. ROC curve showed that miR-106a-5p had high specificity and sensitivity in the diagnosis of psoriasis. The correlation between the serum expression level of miR-106a-5p and PASI was positive. The relative expression levels of IL-17A, IL-22 and TNF-alpha in serum of psoriasis patients were significantly upregulated compared with that in healthy control, and showed positive association with serum miR-106a-5p levels. Cell experiments demonstrated that upregulation of miR-106a-5p could promote cell proliferation, and the levels of IL-22, IL-17A and TNF-alpha were upregulated significantly in M5-induced HaCaT cells.Conclusion: Considering the novel and vital role in psoriasis progression, miR-106a-5p is expected to be a new potent target for treatment of psoriasis. MiR-106-5p was expected to use for more immunity diseases research and therapy.

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Section: Discussionsupporting

confidence: 91%

“…It is commonly used as a model of immune-mediated diseases. The gene expression pro le of the affected skin has made people deeply understand the pathogenesis of psoriasis (5). However, the mechanism leading to psoriasis-speci c mRNA expression is unclear.…”

Section: Introductionmentioning

confidence: 99%

Diagnostic value of miR-106a-5p in patients with psoriasis and its regulatory role in inflammatory responses

Miao

Tong

et al. 2020

Preprint

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“…), tumor necrosis factor-α (TNF-α), interferon-γ (IFN-γ), and transforming growth factor-β (TGF-β) are key players for cell-cell crosstalks (3)(4)(5). As for the non-protein-coding regulatory elements, altered miRNA expression profiles were uncovered in psoriatic skin, blood, and hair samples (6,7), which profoundly influences our knowledge of the driving force and regulatory counterpart in psoriasis.…”

Section: Introductionmentioning

confidence: 99%

miRNAs Flowing Up and Down: The Concerto of Psoriasis

Yang

Wang

2021

Front. Med.

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Psoriasis is a chronic immune-mediated skin disease, whose hallmarks include keratinocyte hyperproliferation and CD4+ T cell subsets imbalance. Dysregulated microRNAs (miRNAs) identified in psoriasis have been shown to affect keratinocyte and T cell functions, with studies on the molecular mechanisms and intrinsic relationships of the miRNAs on the way. Here, we focus on the dysregulated miRNAs that contribute to the two hallmarks of psoriasis with the miRNA target genes confirmed. We review a network, in which, upregulated miR-31/miR-203/miR-155/miR-21 and downregulated miR-99a/miR-125b facilitate the excessive proliferation and abnormal differentiation of psoriatic keratinocytes; upregulated miR-210 and downregulated miR-138 work in concert to distort CD4+ T cell subsets balance in psoriasis. The miRNAs exert their functions through regulating key psoriasis-associated transcription factors including NF-κB and STAT3. Whether flowing up or down, these miRNAs collaborate to promote the development and maintenance of psoriasis.

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“…The first psoriasis-specific miRNAs were published in 2007 8 . With increasing sample sizes, advancing technologies and analytical methods, several differentially expressed miRNAs (DEMs) have since been identified [8][9][10][11][12][13][14] .…”

Section: Introductionmentioning

confidence: 99%

RNA sequencing of a large number of psoriatic patients identifies 131 novel miRNAs and 11 miRNAs associated with disease severity

Solvin

Chawla

Olsen

et al. 2021

Preprint

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BackgroundMicroRNAs are small regulatory molecules that are dysregulated in psoriasis. Despite previous efforts, much is unknown about the regulatory mechanisms of psoriasis genetics and their contributions to disease development and activity.ObjectivesTo globally characterize the miRNAome of psoriatic skin in a large sample of psoriatic cases and controls for increased understanding of psoriasis pathophysiology.MethodsSkin biopsies from psoriatic cases (n=75) and non-psoriatic controls (n=57) were RNA sequenced. Count data was meta-analyzed with a previously published dataset (cases, n=24, controls, n=20), increasing the number of psoriatic cases four-fold from previously published studies. Differential expression analyses were performed comparing lesional psoriatic (PP), non-lesional psoriatic (PN) and control (NN) skin. Further, functional enrichment and cell specific analyses were performed.ResultsWe identified 439 significantly differentially expressed miRNAs (DEMs), of which 131 were novel and 11 were related to disease severity. Meta-analyses identified 20 DEMs between PN and NN, suggesting an inherent change in all psoriatic skin. By integrating the miRNA transcriptome with mRNA target interactions, we identified several functionally enriched terms, including ‘thyroid hormone signaling’, ‘insulin resistance’ and various infectious diseases. Cell specific expression analyses revealed that the upregulated DEMs were enriched in epithelial and immune cells.ConclusionsWe have provided the most comprehensive overview of the miRNome in psoriatic skin to date and identified a miRNA signature related to psoriasis severity. Our results may represent molecular links between psoriasis and related comorbidities and have outlined potential directions for future functional studies to identify biomarkers and treatment targets.

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Integrated microRNA/mRNA expression profiling of the skin of psoriasis patients

Cited by 33 publications

References 62 publications

Diagnostic value of miR-106a-5p in patients with psoriasis and its regulatory role in inflammatory responses

Diagnostic value of miR-106a-5p in patients with psoriasis and its regulatory role in inflammatory responses

miRNAs Flowing Up and Down: The Concerto of Psoriasis

RNA sequencing of a large number of psoriatic patients identifies 131 novel miRNAs and 11 miRNAs associated with disease severity

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