Integrated on-line sample clean-up using cation exchange restricted access sorbent for the LC determination of atropine in human plasma coupled to UV detection

High-performance liquid chromatography coupled with electrospray tandem mass spectrometry (LC/MS/MS) method for the simultaneous determination of diazepam, atropine and pralidoxime in human plasma

“…Additionally, quantification of atropine in the biological matrix has been accomplished by different techniques including HPLC with UV detector [18] or tandem mass spectrometry [19,20] and gas chromatography with mass spectrometry [21]. In addition, atropine was quantified as dl-hyoscyamine equivalents with a radioimmuno assay [22] and a radio receptor assay [23].…”

Restricted access media as a streamlined approach toward on‐line sample preparation: Recent advancements and applications

“…Researchers have demonstrated the benefit of functional groups other than C 18 bonded into the inner core of the silica gel particles, including strong anion exchange (SAX) and strong cation exchange groups (and their weak ion exchange variants) [50][51][52][53]. It is not hard to imagine applications where ion exchange ISP media would be valuable, especially if the trapping step is followed by an RP analytical separation.…”

Profiling of Endogenous Peptides by Multidimensional Liquid Chromatography

“…In 1991, Desilets et al (Desilets, et al, 1991) introduced the restricted access term. Silica based Restricted Access Materials have been developed for the clean-up in bioanalysis; first for low molecular weight compounds in biofluids (Rbeida, et al 2005) and subsequently for biopolymers such as peptides (Wagner et al 2001). Those supports were able to withstand several hundred plasma or serum injections (total volume of 5-7 ml) without losing performance.…”