Integrated Poly(dimethysiloxane) with an Intrinsic Nonfouling Property Approaching “Absolute” Zero Background in Immunoassays

Ma, Hongwei; Wu, Yuanzi; Yang, Xiaoli; Liu, Xing; He, Jianan; Fu, Long; Wang, Jie; Xu, Hongke; Shi, Yi; Zhong, Ruiqin

doi:10.1021/ac101277e

Cited by 34 publications

(35 citation statements)

References 25 publications

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“…Quite a few methods have demonstrated decreased non‐specific binding of proteins on this interface using nanometer‐thick membranes or self‐assembled monolayers (SAMs) 166. A surface‐initiated polymerization strategy was reported to prepare oligo(ethylene glycol) methacrylate‐based anti‐fouling polymer brushes on gold or poly(dimethysiloxane),167, 168 rendering background below the limit of detection of instrument 169…”

Section: Discussionmentioning

confidence: 99%

Nanomaterials for Ultrasensitive Protein Detection

et al. 2013

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The advances of nanomaterials have provided exciting technologies and novel materials for protein detection, based on the unique properties associated with nanoscale phenomena such as plasmon resonance, catalysis and energy transfer. This article reviews a series of nanomaterials including nanoparticles, nanofibers, nanowires, and nanosheets, and evaluates their performances in the application for protein detection, focusing on approaches that realize ultrasensitive detection. Many of these nanomaterials were used to analyze clinically relevant protein biomarkers. Their detection in the picomolar, femtomolar or even zeptomolar regime has been realized, sometimes even with naked‐eye readout. We summarize the detection methods and results according to materials and targets, review the current challenges, and discuss the solution in the context of technological integration such as combining nanomaterials with microfluidics, and classical analytical technologies.

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Section: Discussionmentioning

confidence: 99%

Nanomaterials for Ultrasensitive Protein Detection

et al. 2013

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“…The detection limit of the FEP substrate based protein microarray analysis is much lower than the conventional nitrocellulose membrane and comparable with other state-of-art techniques, which is in the range of 1-10 pg/ml. 29,30,31,32 In conclusion, we have successfully fabricated the protein and peptide microarrays on the FEP substrate for protein analysis. Simple dispensing method was used to deposit capture proteins or peptides onto the polydopamine microspots.…”

Section: Resultsmentioning

confidence: 91%

An ultralow background substrate for protein microarray technology

Feng

Zhang

et al. 2015

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We herein report an ultralow background substrate for protein microarrays. Conventional protein microarray substrates often suffer from non-specific protein adsorption and inhomogeneous spot morphology. Consequently, surface treatment and a suitable printing solution are required to improve the microarray performance. In the current work, we improved the situation by developing a new microarray substrate based on a fluorinated ethylene propylene (FEP) membrane. A polydopamine microspot array was fabricated on the FEP membrane, with proteins conjugated to the FEP surface through polydopamine. Uniform microspots were obtained on FEP without the application of a special printing solution. The modified FEP membrane demonstrated ultralow background signal and was applied in protein and peptide microarray analysis.

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“…In this study, a new solidly supported material, iPDMS membrane, which has a near "zero" background for identification, was used. It achieved high sensitivity in detecting antibodies in serum [17]. These unique features of iPDMS not only simplify data analysis but also reduce nonspecific interactions [18].…”

Section: Discussionmentioning

confidence: 99%

Novel protein chip for the detection of antibodies against infectious bronchitis virus

Yan

Lei

et al. 2018

BMC Vet Res

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BackgroundInfectious bronchitis (IB) caused by the IB virus (IBV) can cause acute damage to chickens around the world. Therefore, rapid diagnosis and immune status determination are critical for controlling IBV outbreaks. Enzyme-linked immunosorbent assays (ELISAs) have been widely used in the detection of IBV antibodies in the early infection and continuous infection of IB because they are more sensitive and quicker than other diagnostic methods.ResultsWe have developed two indirect microarray methods to detect antibodies against IBV: a chemiluminescent immunoassay test (CIT) and a rapid diagnostic test (RDT). IBV nonstructural protein 5 (nsp5) was expressed, purified from Escherichia coli, and used to spot the initiator integrated poly(dimethylsiloxane), which can provide a near “zero” background for serological assays. Compared with the IDEXX IBV Ab Test kit, CIT and RDT have a sensitivity and specificity of at least 98.88% and 91.67%, respectively. No cross-reaction was detected with antibodies against avian influenza virus subtypes (H5, H7, and H9), Newcastle disease virus, Marek’s disease virus, infectious bursal disease virus, and chicken anemia virus. The coefficients of variation of the reproducibility of the intra- and inter-assays for CIT ranged from 0.8 to 18.63%. The reproducibility of RDT was consistent with the original results. The application of the IBV nsp5 protein microarray showed that the positive rate of the CIT was 96.77%, that of the nsp5 ELISA was 91.40%, and that of the RDT was 90.32%. Furthermore, the RDT, which was visible to the naked eye, could be completed within 15 min. Our results indicated that compared with nsp5 ELISA, the CIT was more sensitive, and the RDT had similar positive rates but was faster. Furthermore, the two proposed methods were specific and stable.ConclusionsTwo microarray assays, which were rapid, specific, sensitive, and relatively simple, were developed for the detection of an antibody against IBV. These methods can be of great value for the surveillance of pathogens and monitoring the efficiency of vaccination.

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Integrated Poly(dimethysiloxane) with an Intrinsic Nonfouling Property Approaching “Absolute” Zero Background in Immunoassays

Cited by 34 publications

References 25 publications

Nanomaterials for Ultrasensitive Protein Detection

Nanomaterials for Ultrasensitive Protein Detection

An ultralow background substrate for protein microarray technology

Novel protein chip for the detection of antibodies against infectious bronchitis virus

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