Integrated process design for single‐cell analytical technologies

Love, J. Christopher

doi:10.1002/aic.12413

Cited by 15 publications

(11 citation statements)

References 40 publications

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“…Examples of traits measured for individual cells include protein secretion 31,38–41 , cell-surface markers 8 , motility 42,43 , cytotoxicity 36,44,45 , morphology 46 , proliferation 47 and an endpoint analysis of intracellular protein or transcript expression 48–50 . The spatial confinement of cells in a microsystem allows, therefore, customized bioanalytical processes to be constructed, using modular operations to examine complex functional and phenotypic behaviors 51 . For example, serial measurements of activated T cells can be used to monitor the evolution of secretory states or surface-expressed proteins with time 8,47 .…”

Section: Adding Breadth To Single-cell Analysismentioning

confidence: 99%

Single-cell technologies for monitoring immune systems

et al. 2014

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The complex heterogeneity of cells, and their interconnectedness with each other, are major challenges to identifying clinically relevant measurements that reflect the state and capability of the immune system. Highly multiplexed, single-cell technologies may be critical for identifying correlates of disease or immunological interventions as well as for elucidating the underlying mechanisms of immunity. Here we review limitations of bulk measurements and explore advances in single-cell technologies that overcome these problems by expanding the depth and breadth of functional and phenotypic analysis in space and time. The geometric increases in complexity of data make formidable hurdles for exploring, analyzing and presenting results. We summarize recent approaches to making such computations tractable and discuss challenges for integrating heterogeneous data obtained using these single-cell technologies.

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Section: Adding Breadth To Single-cell Analysismentioning

confidence: 99%

Single-cell technologies for monitoring immune systems

et al. 2014

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“…46 One of the essential features of any technologies for stem cell assays, therefore, would be the capability to isolate and track target stem cells or stem cell colonies in defined compartments for multiparametric analyses. 47 In addition, it is essential not only to decipher the true nature of stem cell behavior, but also to understand the plasticity that is influenced by a heterogeneous environment along with cell-fate decisions and progeny (mechanotransduction, 48 asymmetric cell division, 49 gene regulatory networks, 50 and so on).…”

Section: Current Stem Cell Analysis Methods and Their Limitationsmentioning

confidence: 99%

Technology Advancement for Integrative Stem Cell Analyses

Jeong

Choi

Lee

2014

Tissue Engineering Part B: Reviews

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Scientists have endeavored to use stem cells for a variety of applications ranging from basic science research to translational medicine. Population-based characterization of such stem cells, while providing an important foundation to further development, often disregard the heterogeneity inherent among individual constituents within a given population. The population-based analysis and characterization of stem cells and the problems associated with such a blanket approach only underscore the need for the development of new analytical technology. In this article, we review current stem cell analytical technologies, along with the advantages and disadvantages of each, followed by applications of these technologies in the field of stem cells. Furthermore, while recent advances in micro/nano technology have led to a growth in the stem cell analytical field, underlying architectural concepts allow only for a vertical analytical approach, in which different desirable parameters are obtained from multiple individual experiments and there are many technical challenges that limit vertically integrated analytical tools. Therefore, we propose-by introducing a concept of vertical and horizontal approach-that there is the need of adequate methods to the integration of information, such that multiple descriptive parameters from a stem cell can be obtained from a single experiment.

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“…One class of tools with the potential to provide new opportunities by integrating (16) multiple functions is based on microsystems such as lab-on-a-chip (LOC) devices (17). Lindström et al (17) provide an overview of microdevice-based single-cell tools, such as LOC microfluidics and microwell-based technologies, as well as applications of these technologies.…”

Section: Introductionmentioning

confidence: 99%

“…Lindström et al (17) provide an overview of microdevice-based single-cell tools, such as LOC microfluidics and microwell-based technologies, as well as applications of these technologies. Several reviews focus on the topic of SCA, covering aspects of basic (18, 19), clinical (2), and biotechnological research (3, 16, 20). Furthermore, comprehensive reviews covering different aspects of single-cell “omics” (1, 20) have been published.…”

Section: Introductionmentioning

confidence: 99%

A New Toolbox for Assessing Single Cells

Tsioris¹,

Torres²,

Douce³

et al. 2014

Annu. Rev. Chem. Biomol. Eng.

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Unprecedented access to the biology of single cells is now feasible, enabled by recent technological advancements that allow us to manipulate and measure sparse samples and achieve a new level of resolution in space and time. This review focuses on advances in tools to study single cells for specific areas of biology. We examine both mature and nascent techniques to study single cells at the genomics, transcriptomics, and proteomics level. In addition, we provide an overview of tools that are well suited for following biological responses to defined perturbations with single-cell resolution. Techniques to analyze and manipulate single cells through soluble and chemical ligands, the microenvironment, and cell-cell interactions are provided. For each of these topics, we highlight the biological motivation, applications, methods, recent advances, and opportunities for improvement. The toolbox presented in this review can function as a starting point for the design of single-cell experiments.

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Integrated process design for single‐cell analytical technologies

Cited by 15 publications

References 40 publications

Single-cell technologies for monitoring immune systems

Single-cell technologies for monitoring immune systems

Technology Advancement for Integrative Stem Cell Analyses

A New Toolbox for Assessing Single Cells

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