Integrated system for temperature-controlled fast protein liquid chromatography. III. Continuous downstream processing of monoclonal antibodies

Abstract

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Adsorption of a Humanized Monoclonal Antibody onto Thermoresponsive Copolymer-Grafted Sepharose Fast Flow Sorbents

“…These features should increase process productivity (and thus lower the overall production cost) in the downstream purification of monoclonal antibodies. Similar conclusions have been reached from recent studies with a thermoresponsive rProtein A sorbent packed in a traveling heating zone reactor (THZR) chromatographic system . The results of the current investigation confirmed that the environmental temperature and the composition of the grafted copolymer played an important role in controlling the adsorption behavior.…”

“…Similar conclusions have been reached from recent studies with a thermoresponsive rProtein A sorbent packed in a traveling heating zone reactor (THZR) chromatographic system. 41 The results of the current investigation confirmed that the environmental temperature and the composition of the grafted copolymer played an important role in controlling the adsorption behavior. The higher affinity of the hIgG2 mAb with the negatively charged copolymer-modified surface was found for adsorptions at 50 °C compared with 20 °C due to the enhanced hydrophobic and electrostatic interactions at this higher temperature.…”

Implementations of temperature gradients in temperature-responsive liquid chromatography

“…A different approach to the radial cooling of the column, which more closely mimics a theoretical axial temperature gradient was introduced by the traveling cooling zone device. [28][29][30] This device moves along the column whilst applying radial cooling to only a small section of the column. The axial movement of this device introduces an axial gradient like peak refocusing effect, but this setup has only been demonstrated for a single column continuous operation and might suffer from some significant limitations if applied to separate a sample in a traditional separation.…”