Integrated Transcriptome Analysis Reveals the Impact of Photodynamic Therapy on Cerebrovascular Endothelial Cells

He, Yanyan; Lin, Dehui; Wu, Haigang; Chen, Song; Lu, Taoyuan; Li, Tianxiao; He, Yingkun

doi:10.3389/fonc.2021.731414

Cited by 6 publications

(3 citation statements)

References 56 publications

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“…The target genes ESPL1, KDM6A, and BCORL1 may be engaged in reproductive processes. In many previous studies, ESPL1 (extra spindle pole bodies like 1) have been found involved in cellular mitosis and cell cycle process ( 45 – 47 ). KDM6A (lysine demethylase 6A) belongs to the KDM6 family of histone H3 lysine 27 (H3K27) demethylases, which is an X- linked protein that contains a catalytic Jumonji C (JmjC) domain can facilitate the removal of the methyl group on di- and trimethylated H3K27 (H3K27me2/3), it can not only manifest demethylase activity but also regulate gene expression and enhancer activation independently of its demethylase activity.…”

Section: Discussionmentioning

confidence: 99%

The exploration of miRNAs and mRNA profiles revealed the molecular mechanisms of cattle-yak male infertility

et al. 2022

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Cattle-yak, the first-generation offspring of cattle and yak, inherited many excellent characteristics from their parents. However, F1 male hybrid infertility restricts the utilization of heterosis greatly. In this study, we first compared the testicular tissue histological characteristics of three cattle, three yaks, and three cattle-yak. Then we explored the miRNA profiles and the target functions of nine samples with RNA-seq technology. We further analyzed the function of DE gene sets of mRNA profiles identified previously with GSEA. Testicular histology indicated that the seminiferous tubules became vacuolated and few active germ cells can be seen. RNA-seq results showed 47 up-regulated and 34 down-regulated, 16 up-regulated and 21 down-regulated miRNAs in cattle and yaks compared with cattle-yak, respectively. From the intersection of DE miRNAs, we identified that bta-miR-7 in cattle-yak is down-regulated. Target prediction indicated that the filtered genes especially MYRFL, FANCA, INSL3, USP9X, and SHF of bta-miR-7 may play crucial roles in the reproductive process. With further network analysis and GSEA, we screened such hub genes and function terms, we also found some DE gene sets that enriched in ATP binding, DNA binding, and reproduction processes. We concluded that bta-miR-7 may play an important role in influencing fecundity. Our study provides new insights for explaining the molecular mechanism of cattle-yak infertility.

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Section: Discussionmentioning

confidence: 99%

The exploration of miRNAs and mRNA profiles revealed the molecular mechanisms of cattle-yak male infertility

et al. 2022

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“…It has been shown that ALA-PDT can lead to both down-regulation of NF-kB and enhanced its activation in glioma cells [189,192,193]. Moreover, low-dose PDT with PS Porfimer sodium induces NF-kB of TNF-a/NF-kB pathway in cerebral vascular endothelial cells, and impairment of their function [194]. This effect may be important for the effectiveness of delivering other therapeutics to the tumor after PDT.…”

Section: Nuclear Factor Kbmentioning

confidence: 99%

Molecular Insights into Photodynamic Therapy of Glioma

Aebisher,

Woźnicki,

Bartusik-Aebisher

2024

Preprint

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Cancers, a large and heterogeneous group of malignancies, are becoming an increasingly important cause of premature mortality worldwide. 2% of these are primary CNS tumors, of which 24% are gliomas. These tumors are diverse, but what they have in common is one of the most unfavorable prognoses among all cancers. A patient's future varies depending on the grade of the diagnosed glioma. To date, however, no standard treatment for grade IV has been established. All glioblastomas eventually undergo progression or recurrence. The current standard of treatment, which includes surgical intervention, radiation therapy and chemotherapy, is therefore far from sufficient. Work is constantly underway to discover a new, effective form of glioma therapy. Photodynamic therapy (PDT) may be one of them. It involves the local or systemic application of a photosensitive compound - a photosensitizer (PS), which accumulates in the affected tissues. Photosensitizer molecules absorb light of the appropriate wavelength, initiating activation processes leading to the formation of reactive oxygen species and selective destruction of inappropriate cells. Currently, this method has been approved for the treatment of several cancers. Research focusing on the effective use of PDT in glioma therapy is already underway, with promising results. In our work, we present molecular insights into PDT of glioma. Based on the available literature, we analyze and systematize the impact of various molecules, proteins, transporters and transmitters on the efficacy of PDT and the effect of PDT on their expression. In addition, we highlight gaps in current knowledge and point out directions for future research that may contribute to the efficacy of PDT glioma.

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“…The clinical reports suggest that the PDT intervention may significantly stimulate the endothelial cells of the blood-brain barrier (BBB) for specific responses. Identifying and exploring the pivotal changes in endothelial cells after PDT intervention may provide necessary information to guide the clinical application of PDT on GBM therapy [5]. The study investigated the temporary opening of BBB due to PDT [6,7], initiation of an immune response against tumor cells [8][9][10], and deactivation of tumorassociated macrophages that promote tumor progression [11].…”

Section: Introductionmentioning

confidence: 99%

Effect of Photodynamic Therapy with the Photosensitizer Methylene Blue on Cerebral Endotheliocytes In Vitro

Makarov,

Skobeltsin,

Averchuk

et al. 2024

Photonics

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Background: Microvessels in tumor tissue play a crucial role in meeting the metabolic needs of transformed cells, controlling the entry of xenobiotics into tumor tissue, and regulating local inflammation that promotes metastasis. Methylene blue has photosensitizing properties and can also affect dysfunctional mitochondria. Methods: The study was performed on the primary culture of CECs. The cells underwent photodynamic treatment through 660 nm laser irradiation at a power density of 300 mW/cm2. The MTT, TMRE, and TUNEL assays were used to assess the survival, redox metabolism, mitochondrial activity, and apoptosis of CECs. Additionally, the metabolic activity of cells was evaluated using FLIM by measuring the fluorescence lifetime of NADH and FAD. Results: When CECs were incubated with MB, there was an increase in mitochondrial activity that was dependent on the concentration of MB. Additionally, mitochondrial activity increased when the CECs were exposed to 660 nm laser irradiation at an energy dose of up to 5 J/cm2. Following PDT, a slight shift towards oxidative phosphorylation was observed. Conclusions: In vitro application of MB accumulation or laser irradiation causes a shift in the redox status of CECs towards increased reducing activity, without causing any cell damage. However, the combined action of PS and laser radiation has the opposite effect on the redox status of cells, resulting in an increase in the oxidized form of FAD.

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Integrated Transcriptome Analysis Reveals the Impact of Photodynamic Therapy on Cerebrovascular Endothelial Cells

Cited by 6 publications

References 56 publications

The exploration of miRNAs and mRNA profiles revealed the molecular mechanisms of cattle-yak male infertility

The exploration of miRNAs and mRNA profiles revealed the molecular mechanisms of cattle-yak male infertility

Molecular Insights into Photodynamic Therapy of Glioma

Effect of Photodynamic Therapy with the Photosensitizer Methylene Blue on Cerebral Endotheliocytes In Vitro

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