In this study, we investigated the impact of the lighting environment on psychological perception, physiology, and productivity and then designed lighting control strategies based on the experimental results. The research was conducted in a smart lighting laboratory, and 67 subjects were tested in different illuminances and correlated color temperatures (CCTs). During the experiment, the physiological data of subjects were continuously recorded, while the psychology and productivity results were evaluated by questionnaires and working tests, respectively. The experimental results found that both illuminance and CCT could significantly influence the feeling of comfort and relaxation of the subjects. Warm CCT and higher illuminance (3000 K–590 lux) made subjects feel more comfortable. Productivity reached its maximum value with illuminance above 500 lux and equivalent melanopic lux (EML) higher than 150. The brain-wave and heart-rate changes did not have a close relationship with either illuminance or CCT, but the heart rate slightly increased in the adjustable lighting mode. Regardless of the initial value setting, the subjects preferred intermediate CCT (4200 K) and bright illumination (500 lux) after self-adjustment. Finally, we proposed three comprehensive lighting control strategies based on psychology, productivity, circadian rhythm, and energy-saving.