Integrating photoacoustic microscopy, optical coherence tomography, OCT angiography, and fluorescence microscopy for multimodal imaging

Dadkhah, Arash; Jiao, Shuliang

doi:10.1177/1535370219897584

Cited by 11 publications

(5 citation statements)

References 29 publications

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“…Some technical advancements of needle-transducer-based OC-PAM systems have also been reported such as for dynamic focusing 128,129 and for incorporating additional imaging modalities. 130 However, due to the opaque nature of the needle transducer and knowing that these needle transducers are normally unfocused, alternatives for OC-PAM implementation have been explored. One direct approach is to make the transducer transparent, which was recently demonstrated in an OC-PAM system and applied in different disease or pathological models.…”

Section: System Configurations For Dual Modality Oct-paimentioning

confidence: 99%

Enhanced medical diagnosis for dOCTors: a perspective of optical coherence tomography

et al. 2021

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Significance: After three decades, more than 75,000 publications, tens of companies being involved in its commercialization, and a global market perspective of about USD 1.5 billion in 2023, optical coherence tomography (OCT) has become one of the fastest successfully translated imaging techniques with substantial clinical and economic impacts and acceptance.Aim: Our perspective focuses on disruptive forward-looking innovations and key technologies to further boost OCT performance and therefore enable significantly enhanced medical diagnosis.Approach: A comprehensive review of state-of-the-art accomplishments in OCT has been performed. Results:The most disruptive future OCT innovations include imaging resolution and speed (single-beam raster scanning versus parallelization) improvement, new implementations for dual modality or even multimodality systems, and using endogenous or exogenous contrast in these hybrid OCT systems targeting molecular and metabolic imaging. Aside from OCT angiography, no other functional or contrast enhancing OCT extension has accomplished comparable clinical and commercial impacts. Some more recently developed extensions, e.g., optical coherence elastography, dynamic contrast OCT, optoretinography, and artificial intelligence enhanced OCT are also considered with high potential for the future. In addition, OCT miniaturization for portable, compact, handheld, and/or cost-effective capsule-based OCT applications, home-OCT, and self-OCT systems based on micro-optic assemblies or photonic integrated circuits will revolutionize new applications and availability in the near future. Finally, clinical translation of OCT including medical device regulatory challenges will continue to be absolutely essential.Conclusions: With its exquisite non-invasive, micrometer resolution depth sectioning capability, OCT has especially revolutionized ophthalmic diagnosis and hence is the fastest adopted imaging technology in the history of ophthalmology. Nonetheless, OCT has not been completely exploited and has substantial growth potential-in academics as well as in industry. This applies not only to the ophthalmic application field, but also especially to the original motivation of OCT to enable optical biopsy, i.e., the in situ imaging of tissue microstructure with a resolution approaching that of histology but without the need for tissue excision.

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Section: System Configurations For Dual Modality Oct-paimentioning

confidence: 99%

Enhanced medical diagnosis for dOCTors: a perspective of optical coherence tomography

et al. 2021

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“…The interference light (the OCT signal) was detected by a home-built spectrometer with a line scan CMOS camera (Sprint, Basler) with an A-line rate of up to 140 KHz. For in vivo mouse ear imaging, we used the OCTA function of our recently developed penta-modal imaging system, 25 in which a SLD-based NIR light source with a center wavelength of 840 nm and bandwidth of 100 nm (Broadlighter, Superlume Diodes) was used. In the penta-modal imaging system, the OCT signal was detected by a home-built spectrometer with a fast CCD camera (Aviva EM4, e2V, maximum A-line rate: 70 KHz).…”

Section: System and In Vivo Animal Imagingmentioning

confidence: 99%

Comparative study of optical coherence tomography angiography algorithms for rodent retinal imaging

Dadkhah

Paudel

Jiao

2021

Exp Biol Med (Maywood)

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Optical coherence tomography angiography (OCTA) is a functional extension of optical coherence tomography for non-invasive in vivo three-dimensional imaging of the microvasculature of biological tissues. Several algorithms have been developed to construct OCTA images from the measured optical coherence tomography signals. In this study, we compared the performance of three OCTA algorithms that are based on the variance of phase, amplitude, and the complex representations of the optical coherence tomography signals for rodent retinal imaging, namely the phase variance, improved speckle contrast, and optical microangiography. The performance of the different algorithms was evaluated by comparing the quality of the OCTA images regarding how well the vasculature network can be resolved. Quantities that are widely used in ophthalmic studies including blood vessel density, vessel diameter index, vessel perimeter index, vessel complexity index were also compared. Results showed that both the improved speckle contrast and optical microangiography algorithms are more robust than phase variance, and they can reveal similar vasculature features while there are statistical differences in the calculated quantities.

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“…By adding OCTA function, the system also achieved penta-modal imaging as shown in Figure 5. 41 Generated by the same photons, the images of PAM and CFM are precisely registered in the lateral directions, while the OCT, ODT, and OCTA images are also registered. Registration among the PAM and OCT-based images was achieved by light alignment and synchronization control.…”

Section: Latest Progress For Oct-guided Penta-modal Pam Imaging Technmentioning

confidence: 99%

Integrating photoacoustic microscopy with other imaging technologies for multimodal imaging

Dadkhah

Jiao

2020

Exp Biol Med (Maywood)

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As a hybrid optical microscopic imaging technology, photoacoustic microscopy images the optical absorption contrasts and takes advantage of low acoustic scattering of biological tissues to achieve high-resolution anatomical and functional imaging. When combined with other imaging modalities, photoacoustic microscopy-based multimodal technologies can provide complementary contrast mechanisms to reveal complementary information of biological tissues. To achieve intrinsically and precisely registered images in a multimodal photoacoustic microscopy imaging system, either the ultrasonic transducer or the light source can be shared among the different imaging modalities. These technologies are the major focus of this minireview. It also covered the progress of the recently developed penta-modal photoacoustic microscopy imaging system featuring a novel dynamic focusing technique enabled by OCT contour scan.

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Integrating photoacoustic microscopy, optical coherence tomography, OCT angiography, and fluorescence microscopy for multimodal imaging

Cited by 11 publications

References 29 publications

Enhanced medical diagnosis for dOCTors: a perspective of optical coherence tomography

Enhanced medical diagnosis for dOCTors: a perspective of optical coherence tomography

Comparative study of optical coherence tomography angiography algorithms for rodent retinal imaging

Integrating photoacoustic microscopy with other imaging technologies for multimodal imaging

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