Integrating proteomics and metabolomics approaches to elucidate the ripening process in white Psidium guajava

Monribot‐Villanueva, Juan L.; Altúzar-Molina, Alma; Aluja, Martı́n; Zamora‐Briseño, Jesús Alejandro; Elizalde‐Contreras, José M.; Bautista-Valle, Mirna V.; Santos, Jiovanny Arellano de los; Sánchez-Martínez, Daniela E.; Rivera-Reséndiz, Francisco J.; Vázquez-Rosas-Landa, Mirna; Camacho-Vázquez, Carolina; Guerrero‐Analco, José A.; Ruiz‐May, Eliel

doi:10.1016/j.foodchem.2021.130656

Cited by 28 publications

(17 citation statements)

References 36 publications

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“…However, NCED of ABA biosynthetic pathway were upregulated in the three stages of treatment groups, and during the development of P. cyrtonema seed from corm to seedling emergence, the development of embryo needed to transport nutrients from endosperm to new corm tissue and cold stratification before seedling establishment ( Liao et al, 2021 ). This means that seeds are still not fully mature, and there is a direct correlation between ABA excess production and fruit maturity ( Monribot-Villanueva et al, 2022 ). So the genes of the ABA biosynthetic pathway always upregulated in three stages may be to complete the seed maturation.…”

Section: Discussionmentioning

confidence: 99%

Transcriptomics and metabolomics changes triggered by exogenous 6-benzylaminopurine in relieving epicotyl dormancy of Polygonatum cyrtonema Hua seeds

et al. 2022

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Polygonatum cyrtonema Hua is one of the most useful herbs in traditional Chinese medicine and widely used in medicinal and edible perennial plant. However, the seeds have the characteristics of epicotyl dormancy. In this study, the molecular basis for relieving epicotyl dormancy of P. cyrtonema seeds under exogenous 6-benzylaminopurine (6-BA) treatment was revealed for the first time through transcriptome and metabolomics analysis. We determined the elongation of epicotyl buds as a critical period for dormancy release and found that the content of trans-zeatin, proline, auxin and gibberellin was higher, while flavonoids and arginine were lower in the treatment group. Transcriptome analysis showed that there were significant differences in gene expression in related pathways, and the expression patterns were highly consistent with the change of metabolites in corresponding pathways. Co-expression analysis showed that cytokinin dehydrogenase of P. cyrtonema (PcCKXs) and pelargonidin in flavonoid biosynthesis, as well as L-proline, L-ornithine, and L-citrulline in arginine and proline metabolism form network modules, indicating that they have related regulatory roles. Above all, our findings provide new insight into the exogenous 6-BA relieving epicotyl dormancy of P. cyrtonema seeds.

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Section: Discussionmentioning

confidence: 99%

Transcriptomics and metabolomics changes triggered by exogenous 6-benzylaminopurine in relieving epicotyl dormancy of Polygonatum cyrtonema Hua seeds

et al. 2022

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“…The identification and quantification of individual phenolic compounds in ECn was performed by ultra-high-performance liquid chromatography (Agilent, 1290, Santa Clara, CA, USA) coupled to a triple quadrupole mass spectrometer (Agilent, 6460, Santa Clara, CA, USA; UPLC-MS-QqQ) with a dynamic multiple reaction monitoring method as is shown in Supplementary Table S1 and as we previously described [42,43]. Chromatographic analysis was carried out using a reversed-phase column (Zorbax SB-C18; 1.8 µm, 2.1 × 50 mm; Agilent, Santa Clara, CA, USA) with the column oven temperature at 40 • C. The mobile phase (flow rate of 0.1 mL min −1 ) consisted of water (A) and acetonitrile (B), both containing formic acid (0.1%).…”

Section: Analysis Of Phenolic Compounds By Uplc-ms-qqqmentioning

confidence: 99%

Isolation and Identification of Pennogenin Tetraglycoside from Cestrum nocturnum (Solanaceae) and Its Antifungal Activity against Fusarium kuroshium, Causal Agent of Fusarium Dieback

et al. 2022

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Antifungal assay-guided fractionation of the methanolic crude extract of Cestrum nocturnum (Solanaceae), popular known as ‘lady of the night’, led the isolation and identification of the steroidal saponin named pennogenin tetraglycoside, which was identified for the first time in this plant species by spectroscopic means. The crude extract, fractions and pennogenin tetraglycoside exhibited mycelial growth inhibition of Fusarium solani and F. kuroshium. F. solani is a cosmopolitan fungal phytopathogen that affects several economically important crops. However, we highlight the antifungal activity displayed by pennogenin tetraglycoside against F. kuroshium, since it is the first plant natural product identified as active for this phytopathogen. This fungus along with its insect symbiont known as Kuroshio shot hole borer (Euwallacea kuroshio) are the causal agents of the plant disease Fusarium dieback that affects more than 300 plant species including avocado (Persea americana) among others of ecological relevance. Scanning electron microscopy showed morphological alterations of the fungal hyphae after exposure with the active fractions and 12 phenolic compounds were also identified by mass spectrometry dereplication as part of potential active molecules present in C. nocturnum leaves.

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“…We were surprised/intrigued at the similarity of the gut microbiota of A. striata reared in guava and A. ludens reared in bitter orange, when this similarity would have been expected in the cases of A. striata and A. fraterculus, both attacking guava in nature (A. ludens and A. striata do not share a single host in nature). It is known that when a fruit fly larva develops in guavas, particularly in unripe fruit, it is exposed to high concentrations of secondary metabolites (Gull et al, 2012;Dos Santos et al, 2017;Monribot-Villanueva et al, 2022), especially tannins and other polyphenols, many of them toxic to the larvae (Lee et al, 2010;Birke et al, 2015;Birke and Aluja, 2018). In this sense, while A. striata prefers to oviposit into totally unripe fruit (Birke et al, 2015) that has still not reached full development (stage four or "player marble size"), A. fraterculus prefers stages five or six, green but fully developed and ripe "turning yellow" stages, respectively (Birke et al, 2015;De Oliveira et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

“…striata do not share a single host in nature). It is known that when a fruit fly larva develops in guavas, particularly in unripe fruit, it is exposed to high concentrations of secondary metabolites ( Gull et al, 2012 ; Dos Santos et al, 2017 ; Monribot-Villanueva et al, 2022 ), especially tannins and other polyphenols, many of them toxic to the larvae ( Lee et al, 2010 ; Birke et al, 2015 ; Birke and Aluja, 2018 ). In this sense, while A .…”

Section: Discussionmentioning

confidence: 99%

Bitter friends are not always toxic: The loss of acetic acid bacteria and the absence of Komagataeibacter in the gut microbiota of the polyphagous fly Anastrepha ludens could inhibit its development in Psidium guajava in contrast to A. striata and A. fraterculus that flourish in this host

et al. 2022

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The gut microbiota is key for the homeostasis of many phytophagous insects, but there are few studies comparing its role on host use by stenophagous or polyphagous frugivores. Guava (Psidium guajava) is a fruit infested in nature by the tephritids Anastrepha striata and A. fraterculus. In contrast, the extremely polyphagous A. ludens infests guava only under artificial conditions, but unlike A. striata and the Mexican A. fraterculus, it infests bitter oranges (Citrus x aurantium). We used these models to analyze whether the gut microbiota could explain the differences in host use observed in these flies. We compared the gut microbiota of the larvae of the three species when they developed in guava and the microbiota of the fruit pulp larvae fed on. We also compared the gut microbiota of A. ludens developing in C. x aurantium with the pulp microbiota of this widely used host. The three flies modified the composition of the host pulp microbiota (i.e., pulp the larvae fed on). We observed a depletion of Acetic Acid Bacteria (AAB) associated with a deleterious phenotype in A. ludens when infesting P. guajava. In contrast, the ability of A. striata and A. fraterculus to infest this fruit is likely associated to a symbiotic interaction with species of the Komagataeibacter genus, which are known to degrade a wide spectrum of tannins and polyphenols. The three flies establish genera specific symbiotic associations with AABs. In the case of A. ludens, the association is with Gluconobacter and Acetobacter, but importantly, it cannot be colonized by Komagataeibacter, a factor likely inhibiting its development in guava.

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Integrating proteomics and metabolomics approaches to elucidate the ripening process in white Psidium guajava

Cited by 28 publications

References 36 publications

Transcriptomics and metabolomics changes triggered by exogenous 6-benzylaminopurine in relieving epicotyl dormancy of Polygonatum cyrtonema Hua seeds

Transcriptomics and metabolomics changes triggered by exogenous 6-benzylaminopurine in relieving epicotyl dormancy of Polygonatum cyrtonema Hua seeds

Isolation and Identification of Pennogenin Tetraglycoside from Cestrum nocturnum (Solanaceae) and Its Antifungal Activity against Fusarium kuroshium, Causal Agent of Fusarium Dieback

Bitter friends are not always toxic: The loss of acetic acid bacteria and the absence of Komagataeibacter in the gut microbiota of the polyphagous fly Anastrepha ludens could inhibit its development in Psidium guajava in contrast to A. striata and A. fraterculus that flourish in this host

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