Integration of a dark fermentation effluent in a microalgal-based biorefinery for the production of high-added value omega-3 fatty acids

Chalima, Angelina; Hatzidaki, Angeliki; Karnaouri, Anthi; Topakas, Evangelos

doi:10.1016/j.apenergy.2019.03.058

Cited by 65 publications

(23 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It has also been shown that using birch and spruce hydrolysates as a glucose source, P. tricornutum generated 3.11-and 3.2-times higher EPA productivity, respectively, compared to photoautotrophic cultivation [21]. Another significant candidate is the heterotrophic marine microalgal species Crypthecodinium cohnii, which has been used for commercial production of DHA [168][169][170][171][172][173][174]. C. cohnii is unique due to DHA being almost the only PUFA present in its lipid profile, and DHA content can be up to 65% of the total fatty acids [175].…”

Section: Oleaginous Microalgae and Diatomsmentioning

confidence: 99%

An Overview of Potential Oleaginous Microorganisms and Their Role in Biodiesel and Omega-3 Fatty Acid-Based Industries

et al. 2020

View full text Add to dashboard Cite

Microorganisms are known to be natural oil producers in their cellular compartments. Microorganisms that accumulate more than 20% w/w of lipids on a cell dry weight basis are considered as oleaginous microorganisms. These are capable of synthesizing vast majority of fatty acids from short hydrocarbonated chain (C6) to long hydrocarbonated chain (C36), which may be saturated (SFA), monounsaturated (MUFA), or polyunsaturated fatty acids (PUFA), depending on the presence and number of double bonds in hydrocarbonated chains. Depending on the fatty acid profile, the oils obtained from oleaginous microorganisms are utilized as feedstock for either biodiesel production or as nutraceuticals. Mainly microalgae, bacteria, and yeasts are involved in the production of biodiesel, whereas thraustochytrids, fungi, and some of the microalgae are well known to be producers of very long-chain PUFA (omega-3 fatty acids). In this review article, the type of oleaginous microorganisms and their expertise in the field of biodiesel or omega-3 fatty acids, advances in metabolic engineering tools for enhanced lipid accumulation, upstream and downstream processing of lipids, including purification of biodiesel and concentration of omega-3 fatty acids are reviewed.

show abstract

Section: Oleaginous Microalgae and Diatomsmentioning

confidence: 99%

An Overview of Potential Oleaginous Microorganisms and Their Role in Biodiesel and Omega-3 Fatty Acid-Based Industries

et al. 2020

View full text Add to dashboard Cite

show abstract

“…T66 was cultivated on single VFAs (C1, C2, C3, C4, C5, and C6), which showed that this strain could not utilize C3, C5, and C6 as a substrate if provided at >2 g/L; whereas C2 and C4 could be used efficiently at up to 40 g/L ( Patel et al, 2020c ). The freshwater microalgae C. protothecoides cannot utilize more than 2 g/L of total VFAs ( Fei et al, 2015 ); while some marine microalgae can easily assimilate 30 g/L of acetic acid, 10 g/L of propionate, and 15 g/L of butyric acid ( Chalima et al, 2019 ). C. cohnii was cultivated on various VFAs (C2, C3, and C4) at an initial concentration ranging from 5 to 50 g/L; the highest biomass was synthesized on 30 g/L of acetate, 10 g/L of propionate, and 15 g/L of butyrate, while any further increase in the concentration of these substrates caused growth inhibition ( Chalima et al, 2019 ).…”

Section: Resultsmentioning

confidence: 99%

“…The freshwater microalgae C. protothecoides cannot utilize more than 2 g/L of total VFAs ( Fei et al, 2015 ); while some marine microalgae can easily assimilate 30 g/L of acetic acid, 10 g/L of propionate, and 15 g/L of butyric acid ( Chalima et al, 2019 ). C. cohnii was cultivated on various VFAs (C2, C3, and C4) at an initial concentration ranging from 5 to 50 g/L; the highest biomass was synthesized on 30 g/L of acetate, 10 g/L of propionate, and 15 g/L of butyrate, while any further increase in the concentration of these substrates caused growth inhibition ( Chalima et al, 2019 ). Therefore, it might be possible that the VFAs mixture provided here to SR21 and Cohnii was converted into lipid-free biomass but was not enough to synthesize any lipids.…”

Section: Resultsmentioning

confidence: 99%

Volatile Fatty Acids (VFAs) Generated by Anaerobic Digestion Serve as Feedstock for Freshwater and Marine Oleaginous Microorganisms to Produce Biodiesel and Added-Value Compounds

et al. 2021

View full text Add to dashboard Cite

Given an increasing focus on environmental sustainability, microbial oils have been suggested as an alternative to petroleum-based products. However, microbial oil production relies on the use of costly sugar-based feedstocks. Substrate limitation, elevated costs, and risk of contamination have sparked the search for alternatives to sugar-based platforms. Volatile fatty acids are generated during anaerobic digestion of organic waste and are considered a promising substrate for microbial oil production. In the present study, two freshwater and one marine microalga along with two thraustochytrids were evaluated for their potential to produce lipids when cultivated on volatile fatty acids generated from food waste via anaerobic digestion using a membrane bioreactor. Freshwater microalgae Auxenochlorella protothecoides and Chlorella sorokiniana synthesized lipids rich in palmitic acid (C16:0), stearic acid (C18:0), oleic acid (C18:1), and linoleic acid (C18:2). This composition corresponds to that of soybean and jatropha oils, which are used as biodiesel feedstock. Production of added-value polyunsaturated fatty acids (PUFA) mainly omega-3 fatty acids was examined in three different marine strains: Aurantiochytrium sp. T66, Schizochytrium limacinum SR21, and Crypthecodinium cohnii. Only Aurantiochytrium sp. T66 seemed promising, generating 43.19% docosahexaenoic acid (DHA) and 13.56% docosapentaenoic acid (DPA) in total lipids. In summary, we show that A. protothecoides, C. sorokiniana, and Aurantiochytrium sp. T66 can be used for microbial oil production from food waste material.

show abstract

“…The effluent filtrate obtained from the anaerobic digestion of organic wastes was used as a suitable medium for biomass and lipid production. The same effluent was used before to assess the growth of Crypthecodinium cohnii , a microalgae, that was able to deplete the organic acid content of the effluent after 60 h of fed-batch cultivation [ 53 ].…”

Section: Resultsmentioning

confidence: 99%

Single Cell Oil Production by Oleaginous Yeasts Grown in Synthetic and Waste-Derived Volatile Fatty Acids

et al. 2020

View full text Add to dashboard Cite

Four yeast isolates from the species—Apiotrichum brassicae, Candida tropicalis, Metschnikowia pulcherrima, and Pichia kudriavzevii—previously selected by their oleaginous character and growth flexibility in different carbon sources, were tested for their capacity to convert volatile fatty acids into lipids, in the form of single cell oils. Growth, lipid yields, volatile fatty acids consumption, and long-chain fatty acid profiles were evaluated in media supplemented with seven different volatile fatty acids (acetic, butyric, propionic, isobutyric, valeric, isovaleric, and caproic), and also in a dark fermentation effluent filtrate. Yeasts A. brassicae and P. kudriavzevii attained lipid productivities of more than 40% (w/w), mainly composed of oleic (>40%), palmitic (20%), and stearic (20%) acids, both in synthetic media and in the waste-derived effluent filtrate. These isolates may be potential candidates for single cell oil production in larger scale applications by using alternative carbon sources, combining economic and environmental benefits.

show abstract

Integration of a dark fermentation effluent in a microalgal-based biorefinery for the production of high-added value omega-3 fatty acids

Cited by 65 publications

References 32 publications

An Overview of Potential Oleaginous Microorganisms and Their Role in Biodiesel and Omega-3 Fatty Acid-Based Industries

An Overview of Potential Oleaginous Microorganisms and Their Role in Biodiesel and Omega-3 Fatty Acid-Based Industries

Volatile Fatty Acids (VFAs) Generated by Anaerobic Digestion Serve as Feedstock for Freshwater and Marine Oleaginous Microorganisms to Produce Biodiesel and Added-Value Compounds

Single Cell Oil Production by Oleaginous Yeasts Grown in Synthetic and Waste-Derived Volatile Fatty Acids

Contact Info

Product

Resources

About