Integration of genetically engineered virus nanofibers and fibrin to form injectable fibrous neuron-rich hydrogels and enable neural differentiation

Chen, Yingfan; Liu, Xiangyu; Yang, Mingying; Sun, Weilian; Mao, Chuanbin

doi:10.1039/d2tb01712a

Cited by 7 publications

(5 citation statements)

References 54 publications

(77 reference statements)

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“…Thus, hydrogels have the potential to revolutionize regenerative medicine [168][169][170][171][172]. They are biocompatible, biodegradable, and have several other advantages, making them a versatile tool for clinical use.…”

Section: Addition Of Myoglobinmentioning

confidence: 99%

Diffusion-Limited Processes in Hydrogels with Chosen Applications from Drug Delivery to Electronic Components

et al. 2023

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Diffusion is one of the key nature processes which plays an important role in respiration, digestion, and nutrient transport in cells. In this regard, the present article aims to review various diffusion approaches used to fabricate different functional materials based on hydrogels, unique examples of materials that control diffusion. They have found applications in fields such as drug encapsulation and delivery, nutrient delivery in agriculture, developing materials for regenerative medicine, and creating stimuli-responsive materials in soft robotics and microrobotics. In addition, mechanisms of release and drug diffusion kinetics as key tools for material design are discussed.

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Section: Addition Of Myoglobinmentioning

confidence: 99%

Diffusion-Limited Processes in Hydrogels with Chosen Applications from Drug Delivery to Electronic Components

et al. 2023

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“…Filamentous phage regenerative therapy presents a promising pathway for enhancing stroke recovery, unveiling novel mechanisms for safeguarding and restoring post-stroke brain tissue. Bacteriophages showcase varied capabilities, spanning pathogen clearance, immune modulation, promotion of neurogenesis, and enhancement of vascular function ( 22 – 24 ). These combined effects culminate in neuroprotection, diminished edema, reduction in infarct size, and improved neural functionality.…”

Section: Introductionmentioning

confidence: 99%

“…The modified filamentous bacteriophage promotes the generation of oligodendrocyte precursor cells, stimulates myelin regeneration, significantly enhances neural function and cognitive abilities, and facilitates neural repair. Additionally, it serves as a potential alternative to autologous nerve transplants for restoring extensive nerve damage ( 22 , 26 ). These investigations not only underscore the promising potential of phage therapy in the context of enhancing stroke recovery but also emphasize the need for meticulous parameter optimization encompassing aspects like selection, dosing, administration routes, and temporal considerations to ensure heightened safety and efficacy.…”

Section: Introductionmentioning

confidence: 99%

Harnessing filamentous phages for enhanced stroke recovery

Li,

Yang,

Kong

et al. 2024

Front. Immunol.

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Stroke poses a critical global health challenge, leading to substantial morbidity and mortality. Existing treatments often miss vital timeframes and encounter limitations due to adverse effects, prompting the pursuit of innovative approaches to restore compromised brain function. This review explores the potential of filamentous phages in enhancing stroke recovery. Initially antimicrobial-centric, bacteriophage therapy has evolved into a regenerative solution. We explore the diverse role of filamentous phages in post-stroke neurological restoration, emphasizing their ability to integrate peptides into phage coat proteins, thereby facilitating recovery. Experimental evidence supports their efficacy in alleviating post-stroke complications, immune modulation, and tissue regeneration. However, rigorous clinical validation is essential to address challenges like dosing and administration routes. Additionally, genetic modification enhances their potential as injectable biomaterials for complex brain tissue issues. This review emphasizes innovative strategies and the capacity of filamentous phages to contribute to enhanced stroke recovery, as opposed to serving as standalone treatment, particularly in addressing stroke-induced brain tissue damage.

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“…Many materials have been developed to prepare injectable hydrogel wound dressings, such as natural polymers (gelatin, chitosan, , alginate, , fibrin, , etc.) and synthetic polymers (poly(vinyl alcohol), , poly(acrylic acid), etc.).…”

Section: Introductionmentioning

confidence: 99%

Gelatin-Based Injectable Hydrogels Loaded with Copper Ion Cross-linked Tannic Acid Nanoparticles for Irregular Wound Closure Repair

Zhang,

Chen,

et al. 2023

ACS Appl. Nano Mater.

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It is often difficult to close and repair irregular wounds such as gums with traditional dressings that have a fixed shape, while injectable hydrogels that can be molded in situ offer tremendous advantages in treating complex, large wounds and can better seal or fill a variety of irregular wounds. While the majority of injectable hydrogels developed in current research have poor mechanical properties and biocompatibility and obstacles to clinical applications, this paper reports an injectable hydrogel that can be molded in situ with appropriate mechanical properties, excellent biocompatibility, and antibacterial activity. Under UV irradiation, a methacrylate-gelatin/poly(vinyl alcohol) loaded with copper ion cross-linked tannic acid nanoparticle (GelMA/PVA-TA/Cu2+NPs) precursor solution could rapidly gel within 45 s and adhere stably to the wound tissue by topological adhesion and hydrogen bonding. Through hydrogen bonding and physical entanglement, TA/Cu2+NPs are anchored in the GelMA/PVA interpenetrating network to form the GelMA/PVA-TA/Cu2+NPs composite hydrogel. This injectable hydrogel, which can quickly achieve photocurable in situ molding, can not only achieve closure and repair of irregular wounds but also has appropriate swelling characteristics, absorbing tissue exudate and blocking bacteria, while providing a good moist environment for wounds and continuously promoting wound healing. Cytotoxicity tests and wound healing experiments have shown that GelMA/PVA-TA/Cu2+NPs injectable hydrogels have good biocompatibility and wound closure and repair ability and have promising applications in the treatment of irregularly wounded orifices.

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Integration of genetically engineered virus nanofibers and fibrin to form injectable fibrous neuron-rich hydrogels and enable neural differentiation

Abstract: RGD-displaying phage, fibrin, and neural stem cells (NSCs) are integrated to form a fiber-like hydrogel, which promotes preferential differentiation of NSCs and serves as a nerve graft to repair the long-gap nerve defect by enhanced neurogenesis.

Cited by 7 publications

References 54 publications

Diffusion-Limited Processes in Hydrogels with Chosen Applications from Drug Delivery to Electronic Components

Diffusion-Limited Processes in Hydrogels with Chosen Applications from Drug Delivery to Electronic Components

Harnessing filamentous phages for enhanced stroke recovery

Gelatin-Based Injectable Hydrogels Loaded with Copper Ion Cross-linked Tannic Acid Nanoparticles for Irregular Wound Closure Repair

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