Integration of hepatic lipidomics and transcriptomics reveals the effect of butter-derived ruminant trans fatty acids on lipid metabolism in C57BL/6J mice

Abstract

Integration of multi-omics provides a new perspective for exploring the relationship between butter-derived ruminant trans fatty acids and lipid metabolism.

Low intake of ruminant trans fatty acids ameliorates the disordered lipid metabolism in C57BL/6J mice fed a high-fat diet

“…Interestingly, our previous research suggested that when the intake of R-TFA accounts for 1.3% of the total energy requirement, it might trigger several inflammation pathways in the mice fed a high fat diet, for example, genes TBXAS1 , ALOXE3 and EPHX2 involved in the arachidonic acid metabolic pathway were up-regulated, which exhibited the opposite effects. 22 Besides, several studies indicated that a high dosage of R-TFA caused disordered lipid metabolism in vivo and in vitro , with R-TFA significantly affecting arachidonic acid metabolism and glycerophospholipid metabolism, respectively, which were different from the effects of the low dosage of R-TFA on health. 21,41…”

“…The butter-derived R-TFA were obtained through urea inclusion referred to in our previous method. 22 The fatty acid standard (GLC-463) for analyzing the fatty acid composition was purchased from Nu Chek Co., Ltd (MN, USA). Biochemistry kits for determining the concentrations of serum total cholesterol (TC), total triglyceride (TG), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), aspartate transaminase (AST) and alanine transaminase (ALT) were supplied by Nanjing Jiancheng Bioengineering Institute Co., Ltd (Nanjing, China).…”

“…The hepatic lipid profiles of the C57BL/6J mice ( n = 5) were detected using an ultra-performance liquid chromatography-electrospray ionization-tandem mass spectrometry system (Thermo Fisher Scientific, MA, USA), and the liver lipid samples were separated on a Phenomen Kinetex C18 column (2.1 mm × 100 mm × 1.7 μm, San Jose, CA, USA), with specific parameters as per our previous methods. 22 The relative contents of the potential lipid metabolites were represented as the peak area, and the potential lipid metabolites were screened according to the criteria of variable importance in projection (VIP > 1), P value ( P < 0.05) and fold change (FC ≥ 1.5 or FC ≤ 0.67) of the OPLS-DA models, which were subsequently used for identifying the potential metabolic pathways.…”

“…3,8 In addition, our previous investigations revealed that R-TFA accounting for 4% and 1.3% of the total energy requirement had a detrimental effect on the lipid metabolism in both ApoE −/− mice and C57BL/6J mice fed a high-fat diet. 21,22 Our current research addressed the effect of R-TFA in the daily diet of Chinese residents (0.15% E) on lipid metabolism and explored the potential metabolic pathway involved in the process, which may provide evidence for understanding the health effects of R-TFA and further give a reference for dietary R-TFA intake in residents. In this study, butter-derived R-TFA was obtained through urea inclusion.…”