Integration of magnetic solid phase fishing and off-line two-dimensional high-performance liquid chromatography–diode array detector–mass spectrometry for screening and identification of human serum albumin binders from Radix Astragali

Separation and purification of α-glucosidase inhibitors from Polygonatum odoratum by stepwise high-speed counter-current chromatography combined with Sephadex LH-20 chromatography target-guided by ultrafiltration–HPLC screening

“…By FeCl 3 color reaction, ethyl acetate fraction of P. odoratum contains large quantity of phenolic components. According to our previous reports [16][17][18], the addition of acid to mobile phase could improve remarkably their separation efficiency and peak shape. The ultrafiltration-HPLC method could be used for a rapid screening of bioactive components from complex matrix, particularly for natural products without sample pretreatment.…”

“…It is noted that target-guided isolation of bioactive components has dominant advantageous. For screening methods, we have developed affinity HPLC based assays using ultrafiltration [14], magnetic microspheres [17][18], and surface plasmon resonance [19] to rapidly screen potential ligands in natural products with high sensitivity and selectivity. Among them, ultrafiltration method is easier to be operated without any sophisticated procedures, and a handful of elegant studies have applied this approach to find bioactive components [20][21].…”

Integrated Analysis for Identifying Radix Astragali and Its Adulterants Based on DNA Barcoding

“…Radix Astragali is reported to possess 47 bioactive compounds and has many bioactive properties [32–37]. Various Radix Astragali preparations are commercially available, not only in China as a TCM component, but also in the United States, as dietary supplements [38].…”

Bioactive compounds of Shuang-Huang-Lian prescription and an insight into its binding mechanism by β2-adrenoceptor chromatography coupled with site-directed molecular docking

“…The authors identify baicalin as one of the main bioactive compound of the prescription. Zhang et al [38] applied a facile method based on Fe 3 O 4 @SiO 2 -HSA magnetic solid phase to fish out HSAbinding compounds from Radix astragali. Their results have demonstrated that the immobilized HSA are stable and valid to screen bioactive compounds from complex matrices.…”