Integration of MALDI-TOFMS as high-throughput screening tool into the workflow of combinatorial polymer research

Abstract

The possibilities of an integration of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry ͑MALDI-TOFMS͒ as an high-throughput screening tool into the workflow of combinatorial materials research are discussed. A multiple layer sample preparation technique for MALDI is described in detail and its possibilities of automation and miniaturization are discussed. Automated MALDI sample preparation could be performed within an automated synthesizer robot as well as with an ink-jet printer. The first approach offers the possibility of online reaction monitoring, whereas the second approach gives the opportunity of applications in ultra-high-throughput environments. Moreover, an example of high-throughput screening of a polymerization reaction by MALDI-TOFMS is discussed.

Living Radical Polymerization by the RAFT Process—A First Update

“…This may form from cumyl dithiobenzoate on exposure to light and such disulfides are known to inhibit polymerization until consumed by reaction with initiator-derived radicals. [157,158] Schubert and co-workers [77][78][79]119,159,160] have reported on the development and application of high-throughput techniques in developing conditions for RAFT polymerization of various (meth)acrylate and for characterization of the synthesized polymers. The methods were used to study RAFT …”

Gradient and Microfluidic Library Approaches to Polymer Interfaces

“…By varying spin speed and solution concentration, a discrete film thickness library could be built; moreover, the method can be used to cast discrete compositional libraries of, for example, polymer blends. Another route for discrete film library fabrication is ink jetting, which has been explored by several groups [8][9][10][11]. As with sector spin coating, this technique can be used to create compositional spreads.…”

Sample Target Substrates with Reduced Spot Size for MALDI‐TOF Mass Spectrometry Based on Patterned Self‐Assembled Monolayers

“…An additional important component of the targeted development of highly sensitive MALDI-TOF MS substrates is the application of a suitable matrix, which enables the desorption of the matrix and the analyte as well as an efficient energy transfer of the laser irradiation to the analyte molecules. In our approach a multiple layer spotting approach [20] is applied.…”

“…[20] The hydrophilic hexagonal holes are being used to selectively deposit the matrix by drop casting. The solution automatically assembles into the hexagonal structure (Fig.…”