Integration of Nanomaterials and Bioluminescence Resonance Energy Transfer Techniques for Sensing Biomolecules

Abstract

Bioluminescence resonance energy transfer (BRET) techniques offer a high degree of sensitivity, reliability and ease of use for their application to sensing biomolecules. BRET is a distance dependent, non-radiative energy transfer, which uses a bioluminescent protein to excite an acceptor through the resonance energy transfer. A BRET sensor can quickly detect the change of a target biomolecule quantitatively without an external electromagnetic field, e.g., UV light, which normally can damage tissue. Having been developed quite recently, this technique has evolved rapidly. Here, different bioluminescent proteins have been reviewed. In addition to a multitude of bioluminescent proteins, this manuscript focuses on the recent development of BRET sensors by utilizing quantum dots. The special size-dependent properties of quantum dots have made the BRET sensing technique attractive for the real-time monitoring of the changes of target molecules and bioimaging in vivo. This review offers a look into the basis of the technique, donor/acceptor pairs, experimental applications and prospects.

Theoretical Study on Chemiluminescence of H2O2-dependent Tetrachloro-1,4-benzoquinone

“…In general, in the CL process, thermally activated molecules react on the ground (S 0 ) state to produce excited-state products through a nonadiabatic transition, and these products release energy in the form of light . Currently, both CL and BL are widely utilized as very powerful analytical tools in medical, − chemical, and biotechnological applications. − Most CL and BL processes are O 2 - or H 2 O 2 -dependent . O 2 -dependent CL systems have been extensively explored experimentally − and theoretically. − Some O 2 -dependent BL systems have also undergone experimental − and theoretical − investigations.…”

Nanomaterials-Based Biosensors for COVID-19 Detection—A Review

“…Similarly, TQDs as acceptors in BRET (Bioluminescence Resonance Energy Transfer) systems have been exploited for biosensing, such as glucose detection via CdTe QDs [39] and nucleic acid using carboxylated QDs [40], as well as in in vivo bioimaging applications [41]. Wang et al developed a CuFe 2 O 4 nanoparticles and ZnO composite for detection of toxic methylene blue molecules [42].…”

Biomedical Applications of Electromagnetic Detection: A Brief Review

“…Currently, the stability and reproducibility of biological detection are still poor due to the shortcomings of instability and variability of biologically active units. Nowadays, biological detection will inevitably be greatly developed with the rapid development of biology, informatics, materials science, and microelectronics [160][161][162][163][164]. It is foreseeable that electromagnetic detection will have the following characteristics in the future:…”