Integration of the SV40 promoter/enhancer sequences in an anchorage-independent clonal subline of SV40-infected human keratinocytes

Chen, Guangtian; Baez, Juan M.; Steinberg, Mark L.

doi:10.1016/s0378-1119(96)00432-5

Cited by 3 publications

(2 citation statements)

References 11 publications

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“…The most frequently detected region in SV40 genome in this study is the regulatory region sequence (21/54 in tumour samples). The sequence of this region contains a transcription enhancer element which is able to activate the expression of genes in either orientation (Fanning and Knippers, 1992), and it was shown that the integrated sequence of this region produced fusion transcripts in SV40-infected human keratinocytes (Chen et al, 1996). Therefore, some genes flanking the integrated SV40 sequences are possibly up-regulated to endow cells with enhanced proliferation activity, although we did not confirm whether SV40 genome detected in our samples was integrated into the genomic DNA.…”

Section: mentioning

confidence: 67%

True

et al. 2000

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Recent studies have revealed the evidence for the significance of SV40 genome in human malignancies. In this paper, the presence of SV40-like sequences was investigated in 54 Japanese osteosarcomas in which mutations of the retinoblastoma (Rb), p53, MDM2, and CDK4 genes had been already analysed. Using polymerase chain reaction and Southern hybridization, SV40-like sequences were detected in 25 cases (46.3%). In most cases, only a part of SV40 genome was detected, and the regulatory region containing enhancer sequences was most frequently found (21/54, 38.9%). There was no apparent relationship between the presence of SV40-like sequences and tumour suppressor genes mutations in each tumour. The SV40-like sequences were also detected in peripheral blood cells of substantial proportion of the patients (43.3%), whereas the incidence was much lower (4.7%) in normal healthy controls. This difference is statistically highly significant (P< 0.0001), suggesting that the presence of SV40-like sequences, even if only a part, may play some roles to predispose individuals to osteosarcoma. © 2000 Cancer Research Campaign

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