Workshop Overview: Scientific and Regulatory Challenges for the Reduction, Refinement, and Replacement of Animals in Toxicity Testing,

Purchase, I. F. H.; Botham, Philip A.; Bruner, Leon H.; Flint, Oliver; Frazier, John M.; Stokes, William S.

doi:10.1006/toxs.1998.2450

Cited by 6 publications

(2 citation statements)

References 47 publications

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“…It has been shown that snake venom can induce inflammatory cytokine production and cell death [13]. In the present work, we investigate the cytotoxicity, the cytokine production, and the modulation of activation or regulation markers in human peripheral blood mononuclear cells treated with two concentrations of Crotalus durissus terrificus and Crotalus durissus collilineatus venom.…”

Section: Introductionmentioning

confidence: 99%

Crotalus durissus collilineatusVenom Induces TNF-αand IL-10 Production in Human Peripheral Blood Mononuclear Cells

et al. 2014

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Snake venom has been the subject of numerous studies in an attempt to find properties and biological effects that may be beneficial to man. In this study we evaluated in vitro the effects of Crotalus durissus terrificus (Cdt) and Crotalus durissus collilineatus (Cdc) venom in human peripheral blood mononuclear cells (PBMCs). At 24 h, a significant decrease of viable cells was observed in cells stimulated with the Cdc venom at 0.0005 mg/mL and 0.005 mg/mL compared to the negative control. At 48 h, a significant decrease of viable cells was observed only in cells stimulated with Cdc venom at 0.005 mg/mL. A significant increase of TNF-α and IL-10 was detected 48 hours after culture of PBMC with Cdc, but not with Cdt venom. The expression of CD69 and PD1 (programmed death-1), activation and regulatory cell markers, on CD8+ and CD8− T cells did not change in the presence of Cdt and Cdc venom. Our results suggest the presence of proinflammatory and anti-inflammatory components in the Cdc venom. Further analysis should be done to identify those Cdc venom components as it has been done for the Cdt venom by other authors, indicating that modulatory components are found in the venom of different species of Crotalus snakes.

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Section: Introductionmentioning

confidence: 99%

Crotalus durissus collilineatusVenom Induces TNF-αand IL-10 Production in Human Peripheral Blood Mononuclear Cells

et al. 2014

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“…MIT is a costly procedure involving the inoculation of RABV in animals, making it necessary to look for alternative approaches for rabies confirmatory diagnosis (Corona et al., 2018). Moreover, there is a worldwide trend toward replacing animal inoculation with in vitro methods, replacing animals whenever an alternative method is available; reducing their use by avoiding losses during trials and assays; and refining in order to correctly select the animals and techniques used (Brasil, 2016; Purchase, 1998). In accordance with that, the World Health Organization for Animal Health recommends the use of virus isolation in cell culture whenever possible (WOAH, 2018).…”

Section: Introductionmentioning

confidence: 99%

Laboratory validation of confirmatory tests for rabies diagnosis: Approaches to reduce animal use and facilitate sample collection

Cappelari

Godinho

Silva

et al. 2022

Transbounding Emerging Dis

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Rabies is an encephalitis caused by rabies virus, whose transmission occurs upon contact with infected animals' saliva. The diagnosis is usually performed post-mortem through a direct fluorescent antibody test (DFAT). If the DFAT results are negative, they must be confirmed with an isolation test, usually the mouse inoculation test (MIT), which implies the suffering and death of the animals, high costs and most importantly, up to 28 days to confirm a negative result. Another issue related to rabies diagnosis is the sample collection and storage, which is critical for the rabies virus' RNA genome.Thus, this study aimed to evaluate (i) reverse transcriptase polymerase chain reaction (RT-PCR) and Rabies Tissue Culture Infection Tests (RTCIT) in comparison to DFAT and MIT and (ii) FTA ® cards as an alternative sample collection and preservation method.Eighty animal samples were evaluated through DFAT, RTCIT and RT-PCR; MIT was performed only in DFAT-negative samples. FTA ® cards were evaluated with a subset of 64

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3D QSAR Markov model for drug-induced eosinophilia—theoretical prediction and preliminary experimental assay of the antimicrobial drug G1

González-Dı́az

Tenorio

Castañedo

et al. 2005

Bioorganic & Medicinal Chemistry

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Workshop Overview: Scientific and Regulatory Challenges for the Reduction, Refinement, and Replacement of Animals in Toxicity Testing,

Cited by 6 publications

References 47 publications

Crotalus durissus collilineatusVenom Induces TNF-αand IL-10 Production in Human Peripheral Blood Mononuclear Cells

Crotalus durissus collilineatusVenom Induces TNF-αand IL-10 Production in Human Peripheral Blood Mononuclear Cells

Laboratory validation of confirmatory tests for rabies diagnosis: Approaches to reduce animal use and facilitate sample collection

3D QSAR Markov model for drug-induced eosinophilia—theoretical prediction and preliminary experimental assay of the antimicrobial drug G1

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