Integration of two pKPX-2-derived antibiotic resistance islands in the genome of an ESBL-producing Klebsiella pneumoniae ST3483 from Lebanon

Bitar, Ibrahim; Moussa, Jennifer; Abboud, Edmond; Hrabák, Jaroslav; Tokajian, Sima

doi:10.1016/j.jgar.2019.06.004

Cited by 3 publications

(1 citation statement)

References 3 publications

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“…Draft genomes of Illumina HiSeq sequences were assembled using the CLC Genomics Workbench 10.0.1 (CLC Bio, Aarhus, Denmark) [28]. HGAP4 analysis was used to generate assemblies de novo and data statistics for the original genome data that was measured using Pacbio sequel technology [29]. The Rapid Annotation using the Subsystem Technology (RAST) (https://rast.nmpdr.org/, accessed on 7 August 2021) server was accessed for genome annotation.…”

Section: Whole-genome Sequencing (Wgs) and Analysismentioning

confidence: 99%

Co-Existence of Oxazolidinone Resistance Genes cfr(D) and optrA on Two Streptococcus parasuis Isolates from Swine

Han

Wan

et al. 2023

Antibiotics

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This study was performed to investigate the presence and characteristics of the oxazolidinone resistance genes optrA and cfr(D) in Streptococcus parasuis. In total, 36 Streptococcus isolates (30 Streptococcus suis isolates, 6 Streptococcus parasuis isolates) were collected from pig farms in China in 2020–2021, using PCR to determine the presence of optrA and cfr. Then, 2 of the 36 Streptococcus isolates were further processed as follows. Whole-genome sequencing and de novo assembly were employed to analyze the genetic environment of the optrA and cfr(D) genes. Conjugation and inverse PCR were employed to verify the transferability of optrA and cfr(D). The optrA and cfr(D) genes were identified in two S. parasuis strains named SS17 and SS20, respectively. The optrA of the two isolates was located on chromosomes invariably associated with the araC gene and Tn554, which carry the resistance genes erm(A) and ant(9). The two plasmids that carry cfr(D), pSS17 (7550 bp) and pSS20-1 (7550 bp) have 100% nucleotide sequence identity. The cfr(D) was flanked by GMP synthase and IS1202. The findings of this study extend the current knowledge of the genetic background of optrA and cfr(D) and indicate that Tn554 and IS1202 may play an important role in the transmission of optrA and cfr(D), respectively.

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Section: Whole-genome Sequencing (Wgs) and Analysismentioning

confidence: 99%

Co-Existence of Oxazolidinone Resistance Genes cfr(D) and optrA on Two Streptococcus parasuis Isolates from Swine

Han

Wan

et al. 2023

Antibiotics

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Structures bounded by directly-oriented members of the IS26 family are pseudo-compound transposons.

Harmer

Pong

Hall

2020

Plasmid

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High-Quality Genome Assembly of Pseudopestalotiopsis theae, the Pathogenic Fungus Causing Tea Gray Blight

et al. 2021

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Tea grey blight is one of the most serious foliar diseases of tea tree caused by the plant pathogenic fungus Pseudopestalotiopsis theae which can affect production and quality of tea worldwide. We generated a highly contiguous, 50.41Mbp genome assembly (N50 1.30 Mbp) of P. theae strain CYF27 by combining PacBio long-read and Illumina short-read sequencing technologies. We identified a total of 15,626 gene models, of which 1,038 genes encode putative secreted proteins. The high-quality genome assembly and annotation resource reported here will be useful for the study of fungal infection mechanisms and pathogen-host interaction.

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Integration of two pKPX-2-derived antibiotic resistance islands in the genome of an ESBL-producing Klebsiella pneumoniae ST3483 from Lebanon

Cited by 3 publications

References 3 publications

Co-Existence of Oxazolidinone Resistance Genes cfr(D) and optrA on Two Streptococcus parasuis Isolates from Swine

Co-Existence of Oxazolidinone Resistance Genes cfr(D) and optrA on Two Streptococcus parasuis Isolates from Swine

Structures bounded by directly-oriented members of the IS26 family are pseudo-compound transposons.

High-Quality Genome Assembly of Pseudopestalotiopsis theae, the Pathogenic Fungus Causing Tea Gray Blight

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