Integrative analysis of proteomics and metabolomics of anaphylactoid reaction induced by Xuesaitong injection

Xu, Yubin; Dou, Deqiang; Ran, Xin; Liu, Chunyan; Chen, Jing

doi:10.1016/j.chroma.2015.09.019

Cited by 24 publications

(25 citation statements)

References 19 publications

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“…Functional annotations of the DEPs were employed by KOBAS 2.0 program search against the non-redundant (nr) protein database deposited in NCBI. The database of Kyoto Encyclopedia of Genes and Genomes (KEGG), Gene Ontology (GO), and Cluster of Orthologous Groups (COG) were performed to categorize and group the DEPs 31 , 33 .…”

Section: Methodsmentioning

confidence: 99%

Comparative proteomic analysis provides insight into a complex regulatory network of taproot formation in radish (Raphanus sativus L.)

Xie

Wang

et al. 2018

Hortic Res

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The fleshy taproot of radish is an important storage organ determining its yield and quality. Taproot thickening is a complex developmental process in radish. However, the molecular mechanisms governing this process remain unclear at the proteome level. In this study, a comparative proteomic analysis was performed to analyze the proteome changes at three developmental stages of taproot thickening using iTRAQ approach. In total, 1862 differentially expressed proteins (DEPs) were identified from 6342 high-confidence proteins, among which 256 up-regulated proteins displayed overlapped accumulation in S1 (pre-cortex splitting stage) vs. S2 (cortex splitting stage) and S1 vs. S3 (expanding stage) pairs, whereas 122 up-regulated proteins displayed overlapped accumulation in S1 vs. S3 and S2 vs. S3 pairs. Gene Ontology (GO) and pathway enrichment analysis showed that these DEPs were mainly involved in several processes such as “starch and sucrose metabolism”, “plant hormone signal transduction”, and “biosynthesis of secondary metabolites”. A high concordance existed between iTRAQ and RT-qPCR at the mRNA expression levels. Furthermore, association analysis showed that 187, 181, and 96 DEPs were matched with their corresponding differentially expressed genes (DEGs) in S1 vs. S2, S1 vs. S3, and S2 vs. S3 comparison, respectively. Notably, several functional proteins including cell division cycle 5-like protein (CDC5), expansin B1 (EXPB1), and xyloglucan endotransglucosylase/hydrolase protein 24 (XTH24) were responsible for cell division and expansion during radish taproot thickening process. These results could facilitate a better understanding of the molecular mechanism underlying taproot thickening, and provide valuable information for the identification of critical genes/proteins responsible for taproot thickening in root vegetable crops.

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Section: Methodsmentioning

confidence: 99%

Comparative proteomic analysis provides insight into a complex regulatory network of taproot formation in radish (Raphanus sativus L.)

Xie

Wang

et al. 2018

Hortic Res

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“…However, along with the wide use of red ginseng, adverse drug reactions occur frequently, especially anaphylactoid reactions (ARs). Several red ginseng-containing TCMIs [9] or ginsenoside-containing TCMIs [10] have been shown to cause ARs that may be related to components in red ginseng. Moreover, several cases of ARs induced by oral administration of ginseng or inhalation of ginseng dust have also been recently reported [11, 12].…”

Section: Introductionmentioning

confidence: 99%

Allergens in red ginseng extract induce the release of mediators associated with anaphylactoid reactions

et al. 2017

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BackgroundAnaphylactoid reactions induced by preparations containing red ginseng have been reported. The aim of this study is to evaluate the allergenicity and screen potential allergens in red ginseng extract thoroughly.MethodsRed ginseng extract (RGE) and different fractions of RGE were prepared and evaluated by measuring the degranulation and viability of rat basophilic leukemia 2H3 (RBL-2H3) cells. Potential allergens were screened by RBL-2H3 cell extraction and allergenicity verified in RBL-2H3 cells, mouse peritoneal mast cells, Laboratory of Allergic Disease 2 (LAD2) human mast cells and mice, respectively.Results80% ethanol extract of red ginseng extract induced mast cell degranulation with less cytotoxicity, but 40% ethanol extract could not. Ginsenoside Rd and 20(S)-Rg3 could induce a significant increase in β-hexosaminidase release, histamine release and translocation of phosphatidylserine in RBL-2H3 cells. Ginsenoside Rd and 20(S)-Rg3 also increased β-hexosaminidase release and the intracellular Ca2+ concentration in mouse peritoneal mast cells and LAD2 cells. In addition, histamine levels in serum of mice were elevated dose-dependently.ConclusionsGinsenoside Rd and 20(S)-Rg3 are potential allergens that induce the release of mediators associated with anaphylactoid reactions. Our study could guide optimization of methods associated with Rd/20(S)-Rg3-containing preparations and establishment of quality standards for safe application of Traditional Chinese Medicines.

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“…Mediators such as histamine and β-hexosaminidase released from mast cells and RBL-2H3 cells are stimulated by Xuesaitong injection [67]. Xuesaitong-induced pseudoallergy occurs via direct stimulation, the complement and the kallikrein-kinin pathways [68]. In addition, substances that induce an anaphylactoid effect induce histamine, LTB4, uric acid and other drugs, which have been confirmed to be involved in arginine and proline metabolism [68].…”

Section: Drug-induced Pseudoallergymentioning

confidence: 99%

Drug-Induced Pseudoallergy: A Review of the Causes and Mechanisms

Zhang

Li²,

Shi³

et al. 2017

Pharmacology

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Adverse drug reactions occur frequently and can trigger pseudoallergy, which has become a serious threat to public health. Pseudoallergy is a typical non-immune anaphylactic reaction characterized by the independence of antigen-specific immune responses. In the clinic, pseudoallergy is often elicited by the first dose of medication, and here lies its unpredictability and occasional lethal outcome. However, the mechanisms of pseudoallergy are not well understood. This review focusses on the causes and mechanisms of pseudoallergy induced by drugs. Two categories of mechanisms will be considered, namely, (1) complement activation-related pseudoallergy and (2) mast cell activation-related pseudoallergy. The factors that induce pseudoallergy include opioid drugs, complement activation-related pseudoallergenic drugs, nonsteroidal anti-inflammatory drugs and traditional Chinese medicine injections.

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Integrative analysis of proteomics and metabolomics of anaphylactoid reaction induced by Xuesaitong injection

Cited by 24 publications

References 19 publications

Comparative proteomic analysis provides insight into a complex regulatory network of taproot formation in radish (Raphanus sativus L.)

Comparative proteomic analysis provides insight into a complex regulatory network of taproot formation in radish (Raphanus sativus L.)

Allergens in red ginseng extract induce the release of mediators associated with anaphylactoid reactions

Drug-Induced Pseudoallergy: A Review of the Causes and Mechanisms

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