Integrative analysis of the miRNA–mRNA regulation network in hemocytes of Penaeus vannamei following Vibrio alginolyticus infection

Wang, Feifei; Huang, Lin; Liao, Meiqiu; Dong, Wenna; Liu, Can; Liu, Yuan; Liang, QingJian; Wang, Weina

doi:10.1016/j.dci.2022.104390

Cited by 13 publications

(7 citation statements)

References 71 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Vibrio alginolyticus is a halophilic Gram-negative bacterium, is widely distributed in the sea, inshore and river mouth areas. It is not only the main pathogen of mariculture economic animals, such as oysters, groupers and Penaeus vannamei ( 13 – 15 ), causing huge economic losses to the aquaculture industry and posing a great threat to the breeding environment, but also the key reason for acute gastroenteritis, diarrhea, and even septicemia in humans caused by exposure to contaminated seafood all over the world ( 16 , 17 ). It mainly produces pathogenic effects by producing pathogenic factors that damage host somatic cells and interfere with metabolism.…”

Section: Introductionmentioning

confidence: 99%

Holistic analysis of lysine acetylation in aquaculture pathogenic bacteria Vibrio alginolyticus under bile salt stress

Xiao

Pan

et al. 2023

Front. Vet. Sci.

View full text Add to dashboard Cite

Lysine acetylation modification is a dynamic and reversible post-translational modification, which plays an important role in the metabolism and pathogenicity of pathogenic bacteria. Vibrio alginolyticus is a common pathogenic bacterium in aquaculture, and bile salt can trigger the expression of bacterial virulence. However, little is known about the function of lysine acetylation in V. alginolyticus under bile salt stress. In this study, 1,315 acetylated peptides on 689 proteins were identified in V. alginolyticus under bile salt stress by acetyl-lysine antibody enrichment and high-resolution mass spectrometry. Bioinformatics analysis found that the peptides motif ****A*Kac**** and *******Kac****A* were highly conserved, and protein lysine acetylation was involved in regulating various cellular biological processes and maintaining the normal life activities of bacteria, such as ribosome, aminoacyl-tRNA biosynthesis, fatty acid metabolism, two-component system, and bacterial secretion system. Further, 22 acetylated proteins were also found to be related to the virulence of V. alginolyticus under bile salt stress through secretion system, chemotaxis and motility, and adherence. Finally, comparing un-treated and treated with bile salt stress lysine acetylated proteins, it was found that there were 240 overlapping proteins, and found amino sugar and nucleotide sugar metabolism, beta-Lactam resistance, fatty acid degradation, carbon metabolism, and microbial metabolism in diverse environments pathways were significantly enriched in bile salt stress alone. In conclusion, this study is a holistic analysis of lysine acetylation in V. alginolyticus under bile salt stress, especially many virulence factors have also acetylated.

show abstract

Section: Introductionmentioning

confidence: 99%

Holistic analysis of lysine acetylation in aquaculture pathogenic bacteria Vibrio alginolyticus under bile salt stress

Xiao

Pan

et al. 2023

Front. Vet. Sci.

View full text Add to dashboard Cite

show abstract

“…However, it also is a pathogen of aquaculture animals and leads to massive mortality of aquaculture animals in hatcheries and grow-out farms [1]. V. alginolyticus is the pathogen of vibriosis of several aquaculture animals [2][3][4][5][6][7]. Co-infection of V. alginolyticus and other pathogen bacteria also caused disease in fsh [25,26].…”

Section: Discussionmentioning

confidence: 99%

“…Vibrio alginolyticus is an important pathogen causing vibriosis in the marine culture. In the past several decades, some marine-cultured animals including Lates calcarifer, Sparus aurata, Dicentrarchus labrax, Litopenaeus vannamei, Marsupenaeus japonicas, and Crassostrea gigas sufered serious production losses due to infection by V. alginolyticus [2][3][4][5][6][7]. It has been reported that V. alginolyticus also infected humans and caused gastroenteritis, wound infections, and septicemia and even amputations and death [8].…”

Section: Introductionmentioning

confidence: 99%

Construction and Evaluation of Recombinant Lactococcus lactis Expressing the vscO Gene as a Safe Live Oral Vaccine against Vibrio alginolyticus

Yang

Huang

et al. 2023

Aquaculture Research

View full text Add to dashboard Cite

Vibrio alginolyticus is responsible for significant economic losses in the fish industry. It is urgent to develop a vaccine against V. alginolyticus. In this study, a recombinant Lactococcus lactis expressing the VscO protein of V. alginolyticus (Lc-pMG36e-vscO) is constructed. The Lc-pMG36e-vscO has good hereditary stability. The immune responses in pearl gentian grouper (Epinephelus fuscoguttatus (♀) × E. lanceolatu (♂)) vaccinated with Lc-pMG36e-vscO are evaluated. The titers of specific antibody in the immunized grouper displayed an upward and then downward tendency during the total experiments and reached the peak value of 9.6 at the fourth week after booster immunization. The expression levels of IL-1β, IL-16, TNF-α, and MHC-1α gene in the Lc-pMG36e-vscO group were significantly upregulated after vaccination, indicating that the Lc-pMG36e-vscO could induce an effective and durable immune response. In vivo challenge of the Lc-pMG36e-vscO-immunized fish with V. alginolyticus showed a relative survival percentage of 68%. These results indicated that Lc-pMG36e-vscO could be promising as a candidate oral vaccine against V. alginolyticus.

show abstract

“…V. alginolyticus is one of the major pathogens causing mass mortality of shrimps worldwide, affecting energy metabolism, immune response and development of shrimps (52)(53)(54). Our previous studies have shown that the infection of V. alginolyticus can affect the survival, growth and metabolism of shrimp, induce the production of ROS, apoptosis and autophagy, reduce immune function, and cause DNA damage (22,(55)(56)(57). Therefore, it is necessary to find a drug that can protect shrimp from V. alginolyticus.…”

Section: Discussionmentioning

confidence: 99%

Andrographolide promote the growth and immunity of Litopenaeus vannamei, and protects shrimps against Vibrio alginolyticus by regulating inflammation and apoptosis via a ROS-JNK dependent pathway

et al. 2022

Self Cite

View full text Add to dashboard Cite

Vibrio alginolyticus (V. alginolyticus) is one of the major pathogens causing mass mortality of shrimps worldwide, affecting energy metabolism, immune response and development of shrimps. In the context of the prohibition of antibiotics, it is necessary to develop a drug that can protect shrimp from V. alginolyticus. Andrographolide (hereinafter called Andr), a traditional drug used in Chinese medicine, which possesses diverse biological effects including anti-bacteria, antioxidant, immune regulation. In this study, we investigated the effect of Andr on growth, immunity, and resistance to V. alginolyticus infection of Litopenaeus vannamei (L. vannamei) and elucidate the underlying molecular mechanisms. Four diets were formulated by adding Andr at the dosage of 0 g/kg (Control), 0.5 g/kg, 1 g/kg, and 2 g/kg in the basal diet, respectively. Each diet was randomly fed to one group with three replicates of shrimps in a 4-week feeding trial. The results showed that dietary Andr improved the growth performance and non-specific immune function of shrimps. L. vannamei fed with Andr diets showed lower mortality after being challenged by V. alginolyticus. After 6 h of V. alginolyticus infection, reactive oxygen species (ROS) production, tissue injury, apoptosis, expression of inflammatory factors (IL-1 β and TNFα) and apoptosis-related genes (Bax, caspase3 and p53) were increased in hemocytes and hepatopancreas, while feeding diet with 0.5 g/kg Andr could inhibit the increase. Considering that JNK are important mediators of apoptosis, we examined the influence of Andr on JNK activity during V. alginolyticus infection. We found that Andr inhibited JNK activation induced by V. alginolyticus infection on L. vannamei. The ROS scavenger N-acetyl-l-cysteine (NAC) suppressed V. alginolyticus-induced inflammation and apoptosis, suggesting that ROS play an important role in V. alginolyticus-induced inflammation and apoptosis. Treated cells with JNK specific activator anisomycin, the inflammation and apoptosis inhibited by Andr were counteracted. Collectively, Andr promote the growth and immunity of L. vannamei, and protects shrimps against V. alginolyticus by regulating inflammation and apoptosis via a ROS-JNK dependent pathway. These results improve the understanding of the pathogenesis of V. alginolyticus infection and provide clues to the development of effective drugs against V. alginolyticus.

show abstract

Integrative analysis of the miRNA–mRNA regulation network in hemocytes of Penaeus vannamei following Vibrio alginolyticus infection

Cited by 13 publications

References 71 publications

Holistic analysis of lysine acetylation in aquaculture pathogenic bacteria Vibrio alginolyticus under bile salt stress

Holistic analysis of lysine acetylation in aquaculture pathogenic bacteria Vibrio alginolyticus under bile salt stress

Construction and Evaluation of Recombinant Lactococcus lactis Expressing the vscO Gene as a Safe Live Oral Vaccine against Vibrio alginolyticus

Andrographolide promote the growth and immunity of Litopenaeus vannamei, and protects shrimps against Vibrio alginolyticus by regulating inflammation and apoptosis via a ROS-JNK dependent pathway

Contact Info

Product

Resources

About