Integrative analysis reveals driver long non-coding RNAs in osteosarcoma

Luo, Zhenguo; Xiao, Li; Li, Jing; Dong, Buhuai; Wang, Chunsheng

doi:10.1097/md.0000000000014302

Cited by 7 publications

(8 citation statements)

References 35 publications

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“…This present study demonstrated the prognostic significance of ASMTL-AS1 in patients with EOC. Previous studies also revealed that dysregulation of ASMTL-AS1 would be a useful biomarker of osteosarcoma and bladder cancer 27 28 . Here, we observed a falling expression level of ASMTL-AS1 in EOC different stages, from stage I/II to III/IV.…”

Section: Discussionmentioning

confidence: 97%

Downregulation of lncRNA ASMTL-AS1 in Epithelial Ovarian Cancer Correlates with Worse Prognosis and Cancer Progression

Tang

Liu

et al. 2022

Horm Metab Res

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Given the characters of “Silent killer”, epithelial ovarian cancer (EOC) usually suffered late diagnosis and poor prognosis. Therefore, this study aimed to explore the prognostic significance of ASMTL-AS1 in EOC and investigated the effect of lncRNA ASMTL-AS1 dysregulation on tumor cellular function. ASMTL-AS1 expression was analyzed in 133 EOC tissues and five kinds of cell lines by RT-qPCR. The expression of ASMTL-AS1 was tested for correlation with clinical data using the chi-square test and clinical follow-up using Kaplan-Meier method with log-rank test. Further, the prognostic parameters in predicting EOC overall survival were assessed by using multivariate Cox proportional hazards analysis. In vitro assays, including MTT assay and transwell assay, were conducted using EOC cell lines with overexpression of ASMTL-AS1. In tumorous tissues and cell lines, ASMTL-AS1 was lowly expressed compared with normal ones. This downregulation was associated with the advanced FIGO stage, positive ascites cytology, and lymph node. In particular, low levels of ASMTL-AS1 were revealed to have a high prognostic impact on EOC. ASMTL-AS1 overexpression strongly decreased cell proliferation, migration, and invasion in vitro partly by moderating miR-1228-3p. This study demonstrates a significant role for lowly expressed ASMTL-AS1 in EOC allowing for the prediction of prognosis for EOC. Considering that ASMTL-AS1 is strongly involved in cell growth and invasion, ASMTL-AS1 may be a promising marker for EOC prognosis and therapy

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Section: Discussionmentioning

confidence: 97%

Downregulation of lncRNA ASMTL-AS1 in Epithelial Ovarian Cancer Correlates with Worse Prognosis and Cancer Progression

Tang

Liu

et al. 2022

Horm Metab Res

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“…Similarly, ASMTL-AS1 was significantly decreased in gastric tissues and cells when compared with the normal ones. Due to the role of ASMTL-AS1 in mediating cell functions and predicting overall survival [ 14 , 15 , 26 ], we also hypothesized that this lncRNA may play a role as a tumor suppressor in gastric cancer and may have the potential as a prognostic marker. Therefore, the distinguishing ability of ASMTL-AS1 between survivors and non-survivors of patients also was analyzed by ROC curves, and the cutoff value was thus determined.…”

Section: Discussionmentioning

confidence: 99%

LncRNA ASMTL-AS1/microRNA-1270 differentiate prognostic groups in gastric cancer and influence cell proliferation, migration and invasion

Song

Wang

2022

Bioengineered

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The objective of this study was to determine the expression levels of ASMTL-AS1 and miR-1270 in gastric cancer, and to explore whether ASMTL-AS1 and miR-1270 is associated with cancer prognosis and progression or not. ASMTL-AS1 and miR-1270 expression were quantified in gastric cancer tissues and adjacent normal tissues (n = 167) and cell lines. The potential of ASMTL-AS1 and miR-1270 as prognostic biomarkers was evaluated by the receiver operating characteristic (ROC) curve, Kaplan-Meier, and multivariate Cox regression analyses. The binding between ASMTL-AS1 and miR-1270 was verified by the Luciferase reporter assay and RNA pull-down assay. Functional roles of ASMTL-AS1/miR-1270 on cells were investigated in HGC-27 and NCI-N87 cells by MTS viability, Transwell migration, and Matrigel invasion assay. ASMTL-AS1 was significantly downregulated while miR-1270 was upregulated in gastric cancer tissues as compared with normal tissue and cell lines. According to the studies, ASMTL-AS1 and miR-1270 were related to unfavorable clinical parameters, such as the advanced TNM stage. Downregulated ASMTL-AS1 and upregulated miR-1270 were associated with reduced 5‐year overall survival. Functional studies suggested that ASMTL-AS1 inhibits proliferation, migration, and invasion of HGC-27 and NCI-N87 cells by regulation of miR-1270. In summary, ASMTL-AS1 and miR-1270 are associated with poor prognosis of patients with gastric cancer. ASMTL-AS1 inhibited gastric cancer progression by regulating miR-1270. Therefore, ASMTL-AS1/miR-1270 may be a potential prognostic biomarker and novel strategy for gastric cancer targeted therapy.

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“…The expression pattern of lncRNAs and their function in tumorigenesis is well investigated in osteosarcoma ( Table 1 ). A recent transcriptome profiling study based on the TARGET data detected a total of 13,903 expressed lncRNAs and their integrative gene expressions and SCNA analysis revealed 167 novel driver lncRNAs (including 2 previously reported lncRNA PVT1 and ZFAS1) to be associated with osteosarcoma ( Luo et al, 2019 ). Another microarray analysis noted 25,733 expressed lncRNAs in human osteosarcoma, among which 403 were upregulated and 798 were downregulated when comparing osteosarcoma tissues to adjacent normal tissues ( Li et al, 2018 ).…”

Section: Long Non-coding Rnasmentioning

confidence: 99%

Navigating the genomic instability mine field of osteosarcoma to better understand implications of non-coding RNAs

Fatema¹,

Larson²,

Barrott³

2022

Biocell

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Osteosarcoma is one of the most genomically complex cancers and as result, it has been difficult to assign genomic aberrations that contribute to disease progression and patient outcome consistently across samples. One potential source for correlating osteosarcoma and genomic biomarkers is within the non-coding regions of RNA that are differentially expressed. However, it is unsurprising that a cancer classification that is fraught with genomic instability is likely to have numerous studies correlating non-coding RNA expression and function have been published on the subject. This review undertakes the formidable task of evaluating the published literature of noncoding RNAs in osteosarcoma. This is not the first review on this topic and will certainly not be the last. The review is organized with an introduction into osteosarcoma and the epigenetic control of gene expression before reviewing the molecular function and expression of long non-coding RNAs, circular RNAs, and short non-coding RNAs such as microRNAs, piwi RNAs, and short-interfering RNAs. The review concludes with a review of the literature and how the biology of non-coding RNAs can be used therapeutically to treat cancers, especially osteosarcoma. We conclude that non-coding RNA expression and function in osteosarcoma is equally complex to understanding the expression differences and function of coding RNA and proteins; however, with the added lens of both coding and non-coding genomic sequence, researchers can begin to identify the patterns that consistently associate with aggressive osteosarcoma.

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Integrative analysis reveals driver long non-coding RNAs in osteosarcoma

Abstract: Supplemental Digital Content is available in the text

Cited by 7 publications

References 35 publications

Downregulation of lncRNA ASMTL-AS1 in Epithelial Ovarian Cancer Correlates with Worse Prognosis and Cancer Progression

Downregulation of lncRNA ASMTL-AS1 in Epithelial Ovarian Cancer Correlates with Worse Prognosis and Cancer Progression

LncRNA ASMTL-AS1/microRNA-1270 differentiate prognostic groups in gastric cancer and influence cell proliferation, migration and invasion

Navigating the genomic instability mine field of osteosarcoma to better understand implications of non-coding RNAs

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