Integrative transcriptomic and genomic analysis of odorant binding proteins and chemosensory proteins in aphids

Wang, Q.; Zhou, Jing‐Jiang; Liu, J.-T.; Guixiu, Huang; Xu, Weina; Zhang, Qiaoqin; Chen, J.-L.; Zhang, Y.-J.; Li, X.-C.; Gu, Siyi

doi:10.1111/imb.12513

Cited by 49 publications

(45 citation statements)

References 71 publications

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“…Based on the number of conserved cysteines, the 10 AglyOBPs were divided into 3 subfamilies: Classic OBPs (AglyOBP2, 3, 4, 7, 8, 9, and 10), Plus-C OBPs (AglyOBP5 and 6), and Minus-C OBPs (AglyOBP11). Compared with previous aphid studies, identifying only 13, 9, 9 and 15 OBPs in Aphis gossypii, Sitobion avenae, Myzus persicae and Acyrthosiphon pisum, respectively (Zhou et al, 2010;Gu et al, 2013;Xue et al, 2016;Wang et al, 2019), we found the members of subfamilies were significantly similar between diverse aphid species. For example, although AgosOBP2-10 belong to classic OBPs, all OBP5 and OBP6 of other diverse aphid species belong to Plus-C OBPs, which indicates that Plus-C OBPs must have evolved before the divergence of the aphids from the dipterans (Zhou et al, 2010).…”

Section: Discussioncontrasting

confidence: 81%

Identification and expression profiles analysis of odorant‐binding proteins in soybean aphid, Aphis glycines (Hemiptera: Aphididae)

Bi¹,

Liu²,

Li³

et al. 2019

Insect Science

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The soybean aphid, Aphis glycines, is an extreme specialist and an important invasive pest that relies on olfaction for behaviors such as feeding, mating, and foraging. Odorant‐binding proteins (OBPs) play a vital role in olfaction by binding to volatile compounds and by regulating insect sensing of the environment. In this work we used rapid amplification of complementary DNA ends technology to identify and characterize 10 genes encoding A. glycines OBPs (AglyOBPs) belonging to 3 subfamilies, including 4 classic OBPs, 5 Plus‐C OBPs, and one Minus‐C OBP. Quantitative real‐time polymerase chain reaction demonstrated variable specific expression patterns for the 10 genes based on developmental stage and aphid tissue sampled. Expression levels of 7 AglyOBPs (2, 3, 4, 5, 7, 9, and 10) were highest in the 4th instar, indicating that the 4th nymphal instar is an important developmental period during which soybean aphids regulate feeding and search for host plants. Tissue‐specific expression results demonstrated that AglyOBP2, 7, and 9 exhibited significantly higher expression levels in antennae. Meanwhile, ligand‐binding analysis of 5 OBPs demonstrated binding of AglyOBP2 and AglyOBP3 to a broad spectrum of volatiles released by green leaf plants, with bias toward 6‐ to 8‐carbon chain volatiles and strong binding of AglyOBP7 to trans‐β‐farnesene. Taken together, our findings build a foundation of knowledge for use in the study of molecular olfaction mechanisms and provide insights to guide future soybean aphid research.

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Section: Discussioncontrasting

confidence: 81%

Identification and expression profiles analysis of odorant‐binding proteins in soybean aphid, Aphis glycines (Hemiptera: Aphididae)

Bi¹,

Liu²,

Li³

et al. 2019

Insect Science

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“…4A ). This phenomenon is also observed in other insect species, such as Drosophila melanogaster Meigen (Diptera: Drosophilidae), Apis mellifera L. (Hymenoptera: Apidae), B. mori , and Myzus persicae (Sulzer) (Homoptera: Aphididae) ( Hekmat-Scafe et al 2002 , Forêt and Maleszka 2006 , Gong et al 2009 , Wang et al 2019 ). Among insect OBP s, GOBP s and PBP s are lepidopteran specific, and the location of GOBP and PBP genes on the same genomic scaffold has been found in several lepidopterans, including B. mori , M. sexta , Danaus plexippus (L.) (Lepidoptera: Nymphalidae), and S. frugiperda (Smith) (Lepidoptera: Noctuidae) ( Gong et al 2009 , Yasukochi et al 2018 ).…”

Section: Discussionmentioning

confidence: 56%

Identification and Expression Profiles of 14 Odorant-Binding Protein Genes From Pieris rapae (Lepidoptera: Pieridae)

Jiang

et al. 2020

Journal of Insect Science

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The small white butterfly, Pieris rapae (L.), is an important insect pest of Brassica crops. This species utilize olfactory cues to find their hosts and mates. However, the molecular mechanism underlying the olfactory perception in this species remains unclear. Here, we identified 14 odorant-binding proteins (OBP) genes—essential for insect olfaction—in P. rapae by exploring a previously published transcriptome dataset. Proteins encoded by all of these genes contain N-terminal signal peptides and six positionally conserved cysteine residues, which are characteristic of insect OBPs. These OBPs displayed high amino acid identity with their respective orthologs in other lepidopterans, and several conserved motifs were identified within these OBPs. Phylogenetic analysis showed that these OBPs were well segregated from each other and clustered into different branches. PrapOBP1 and PrapOBP2 were clustered into the ‘general odorant-binding protein’ clade, and PrapOBP3 and PrapOBP4 fall into the ‘pheromone-binding protein’ clade. The 14 OBP genes were located on seven genomic scaffolds. Of these, PrapOBP1, 2, 3, and 4 were located on scaffold332, whereas PrapOBP5, 6, 7, 8, and 9 were located on scaffold116. Ten of the 14 genes had antenna-biased expression. Of these, PrapOBP1, 2, 4, and 13 were enriched in male antennae, whereas PrapOBP7 and PrapOBP10 were female-biased. Our findings suggest that these OBPs may be involved in olfactory communication. To the best of our knowledge, this is the first report on the identification and characterization of OBPs in P. rapae, and our findings provide a solid foundation for studying the functions of these genes.

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“…persicae and A . pisum species . Thus, these derivatives might have the broad‐spectrum effective responses across aphid species, suggesting a widely conserved interaction and potential for management.…”

Section: Resultsmentioning

confidence: 99%

Bioactivities of synthetic salicylate‐substituted carboxyl (E)‐β‐Farnesene derivatives as ecofriendly agrochemicals and their binding mechanism with potential targets in aphid olfactory system

Qin

Yang

Wang

et al. 2020

Pest Management Science

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BACKGROUND: The aphid alarm pheromone, (E)-⊎-farnesene (E⊎F), is a natural product secreted from the aphid cornicle as a signal to warn companions of danger. Odorant binding proteins (OBPs) are the vital targets in insect signal transduction pathways. To improve bioactivity of E⊎F as more economic and stable aphid control agents, E⊎F derivatives containing an active substructure, salicylic acid moiety, were designed, synthesized, and evaluated for their bioactivities in a structure-function study under laboratory conditions. RESULTS: E⊎F derivatives, (E)-3,7-dimethylocta-2,6-dien-1-yl-2-hydroxy-3-methylbenzoate and (E)-3,7-dimethylocta-2,6-dien-1-yl-2-hydroxy-3-methoxybenzoate showed outstanding aphid-repellent activity at a dose of 5 ∼g against Acyrthosiphon pisum (repellency proportions of 67.3% and 71.2%, respectively) and Myzus persicae (repellency proportions of 80.0% and 74.4%, respectively) in laboratory. E⊎F and most of its derivatives bound strongly to ApisOBP9 with a higher affinity than those of the reported potential targets AphisOBP3 and ApisOBP7. The binding affinities to these three ApisOBPs were generally consistent with the in vivo aphid-repellent activity. A molecular docking study suggested that the hydrophobic effect was crucial for the interactions between the derivatives and the OBPs. CONCLUSION: New E⊎F derivatives containing salicylic acid moiety and their repellent activity, binding mechanism with three potential OBPs are presented. A new OBP, ApisOBP9, was characterized as a potential E⊎F and E⊎F derivatives binding protein for the first time. The hydrophobic nature of these analogues is responsible for their activity. Two analogues 3b and 3e with outstanding aphid-repellent activity could be new leads for aphid control agents.

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Integrative transcriptomic and genomic analysis of odorant binding proteins and chemosensory proteins in aphids

Cited by 49 publications

References 71 publications

Identification and expression profiles analysis of odorant‐binding proteins in soybean aphid, Aphis glycines (Hemiptera: Aphididae)

Identification and expression profiles analysis of odorant‐binding proteins in soybean aphid, Aphis glycines (Hemiptera: Aphididae)

Identification and Expression Profiles of 14 Odorant-Binding Protein Genes From Pieris rapae (Lepidoptera: Pieridae)

Bioactivities of synthetic salicylate‐substituted carboxyl (E)‐β‐Farnesene derivatives as ecofriendly agrochemicals and their binding mechanism with potential targets in aphid olfactory system

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