Integrative transcriptomic and metabolomic analysis in mice reveals the mechanism by which ginseng stem-leaf saponins enhance mucosal immunity induced by a porcine epidemic diarrhea virus vaccination

Su, Fei; Xue, Yin; Ye, Shiyi; Yu, Bin; Li, Junxing; Xu, Lihua; Yuan, Xiufang

doi:10.1016/j.vaccine.2023.09.017

Cited by 1 publication

(1 citation statement)

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“…Ginsenosides are small-molecule metabolites composed of the triterpenoid parent nucleus and various glycosyl units [20], with masses ranging from 350 to 1500 Da [21]. The leaves of P. ginseng, exhibiting immune-boosting properties and suppressing oxidative stress-related apoptosis [22,15], have a rich variety of ginsenosides [23,24]. Therefore, we use the P. ginseng leaves (PGL) as the research object for instrument performance comparison by analyzing the contained ginsenosides.…”

Section: Introductionmentioning

confidence: 99%

Evaluation and comparison of liquid chromatography/high‐resolution mass spectrometry platforms for the separation and characterization of ginsenosides from the leaves of Panax ginseng

Li,

Zou,

Cheng

et al. 2024

J of Separation Science

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The measurement of data repeatability in small‐molecule metabolites acquired within and among different liquid chromatography‐mass spectrometry (LC‐MS) platforms is crucial for data sharing or data transfer in natural products research. This work was designed to investigate and evaluate the separation and detection performance of three commercial high‐resolution LC‐MS platforms (e.g., Agilent 6550 QTOF, Waters Vion IM‐QTOF, and Thermo Scientific Orbitrap Exploris 120) using 68 ginsenoside references and the extract of Panax ginseng leaf. The retention time (tR), measured on these three platforms (under the same chromatography condition), showed good stability in different concentration tests, and within/among different instruments for both intra‐day and inter‐day precision examinations. Correlation in tR of ginsenosides was also highly determined on these three platforms. In spite of the different mass analyzers involved, these three platforms gave the accurate mass determination ability, especially enhanced resolution gained because of the ion mobility (IM) separation facilitated by IM‐quadrupole time‐of‐flight. The current study has systematically evaluated the separation and MS detection performance enabled by three high‐resolution LC‐MS platforms taking ginsenosides as the template, and the reported findings can benefit the researchers for the selection of analytical platforms and the purpose of data sharing or data transfer.

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