Intensification of p-coumaric acid heterologous production using extractive biphasic fermentation

Developing Multi-Copy Chromosomal Integration Strategies for Heterologous Biosynthesis of Caffeic Acid in Saccharomyces cerevisiae

“…The high number of endogenous decarboxylases in S. cerevisiae such as phenylacrylic acid decarboxylase (PAD1) and ferulic acid decarboxylase (FDC1) have been shown to possess p -CA decarboxylase activity ( Mukai et al, 2010 ; Koopman et al, 2012 ). And the decreased p -CA titers are in accordance with Combes et al (2021) who have found the limitation of p -CA accumulation in fermentation broth and observed 4-vinylphenol as the main p -CA degradation product. Thus, apart from the conversion to caffeic acid, the decreased p -CA titers may also result from p -CA degradation being faster than its synthesis in rich medium.…”

From Biomass-Derived p-Hydroxycinnamic Acids to Novel Sustainable and Non-Toxic Phenolics-Based UV-Filters: A Multidisciplinary Journey

“…Indeed, since hydroxycinnamic acids are hydrophobic compounds, their solubility in aqueous solutions such as fermentation media is low ( Mota et al, 2008 ), they are also known to be antimicrobial agents ( Alves et al, 2013 ; Pernin et al, 2019 ), hence, their accumulation in the broth leads to toxicity towards the producer microorganism (inhibition). In a previous study, a different strategy was proposed to simultaneously address all these concerns, by focusing on the intensification through process optimization and, more precisely, on the implementation of an in situ product recovery (ISPR) ( Combes et al, 2021 ). The continuous recovery of heterologously synthesized p -CA through liquid-liquid extraction was implemented ( Figure 2 ), enabling the detoxification of the medium and the enhancement of accumulation limits.…”

Bioprocess intensification: A route to efficient and sustainable biocatalytic transformations for the future

“…Liquid-liquid extraction (LLE) in biotechnology is by no means a new technology; it has been widely employed as a downstream purification technique at commercial scale in Podbielniak centrifugal extractors for penicillin recovery in the pharmaceutical industry [9], which are the one of earliest examples of bioprocess intensification technology. However, the more recent developments in LLE have been concerned with its incorporation in-situ within fermentation systems using conventional organic solvents [121,123,124] or more novel solvents e.g. ionic liquids [125,126].…”

“…The fact that different types of microorgansims exhibit a range of toxicity behaviour vis-à-vis a certain class of solvent [123] means that solvent screening on a case-by-case basis is an important step in applying LLE to biocatalytic systems. Provided biocompatibility between the microorganisms and the solvent can be achieved, the in-situ LLE extraction technique can result in considerable improvements in product yield [121,122,124]. Even greater processing benefits for LLE as an ISPR technique in whole cell biocatalysis have been demonstrated in continuous flow reactors.…”