Inter- and intra-observer variability of time-lapse annotations

Sundvall, Linda; Ingerslev, Hans Jakob; Knudsen, Ulla Breth; Kirkegaard, Kirstine

doi:10.1093/humrep/det366

Cited by 132 publications

(93 citation statements)

References 19 publications

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“…However, the lack of information in this abstract regarding patient and sperm characteristics and cycle outcome prevents us from drawing any firm conclusion. Although annotating embryo development up to the blastocyst stage with TLM devices has been shown to be feasible with a fair reproducibility by trained operators [10], evaluating morula and the blastocyst stages remains a challenging issue. In order to minimize the operatordependent variation in blastocyst annotation, two trained embryologists performed this analysis.…”

Section: Discussionmentioning

confidence: 99%

“…Indeed, morphokinetics has been shown to be more reproducible [10] and more accurate in predicting embryo quality and subsequent implantation than morphology alone [22], which suffers from limited performance. As the few other studies comparing embryo development according to sperm origin have been based on conventional morphology assessment, their conclusions should be treated with caution.…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, all data are based on conventional embryo quality assessment with morphology, this technique being known to suffer from moderate sensitivity, moderate specificity [7], and inter/intra-observer variability [8]. The recent introduction of time-lapse monitoring (TLM) systems, providing stable incubation conditions and continuous follow-up of embryo development with exact measurement of all cleavage timings, has begun a new era in the field of embryology, allowing the implementation of a more accurate and reproducible embryo quality assessment method based on morphokinetics [9,10]. TLM can thus be used to compare embryo development in ICSI cycles in terms of sperm origin more accurately than conventional morphology.…”

Section: Introductionmentioning

confidence: 99%

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Does sperm origin affect embryo morphokinetic parameters?

Lammers

Reignier

Splingart

et al. 2015

J Assist Reprod Genet

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Purpose The purpose of our study was to use time-lapse in order to evaluate the impact of sperm origin (fresh ejaculate or surgically retrieved) on embryo morphokinetic parameters and clinical outcome in intracytoplasmic sperm injection (ICSI) cycles. Methods This retrospective monocentric study was conducted in 485 unselected couples undergoing 604 ICSI cycles with embryo culture in the Embryoscope®. Among them, 445 couples underwent ICSI cycle with fresh ejaculated sperm and 40 with surgically retrieved sperm (26 with testicular sperm and 14 with epididymal sperm). Embryo morphokinetic parameters and clinical cycle outcome were compared between fresh ejaculated sperm and surgically retrieved sperm. A subgroup analysis was also conducted between testicular and epididymal sperm ICSI cycles.Results Clinical outcome was comparable between groups according to sperm origin. Although most early morphokinetic parameters were comparable between ejaculated and surgical sperm groups, a few parameters were significantly different between both groups, but with a considerable overlap in their distribution. Late cellular events occurred significantly later in the surgical sperm group than in the ejaculated sperm group. Conclusions Morphokinetic analysis did not allow us to identify clinically relevant differences between fresh ejaculate and surgically retrieved sperm groups. Further studies are needed, especially concerning the relationship between sperm origin and late morphokinetic parameters, such as blastocyst development.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Does sperm origin affect embryo morphokinetic parameters?

Lammers

Reignier

Splingart

et al. 2015

J Assist Reprod Genet

View full text Add to dashboard Cite

show abstract

“…Recent studies reported information on the association of the type of fertilization [22], the IVF protocol used [23], female obesity [24], and smoking [25] on embryo kinetics and development. Interestingly, the sensitivity and specificity of the modality in terms of predicting the progression to the blastocyst stage was >90 % by measuring parameters at day 2 after fertilization, before embryonic genome activation [26,27], while the bad prognosis of good-performing but unviable embryos reaches a specificity of 100 % [28]; moreover, the area under the receiver operating characteristic curve has been reported to be 0.74 for live birth [29] with high intra-and inter-observer correlation [30].…”

Section: Introductionmentioning

confidence: 99%

Morphokinetic parameters of early embryo development via time lapse monitoring and their effect on embryo selection and ICSI outcomes: a prospective cohort study

Siristatidis

Komitopoulou

Makris

et al. 2015

J Assist Reprod Genet

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Purpose To compare the outcomes of embryos selected via time lapse monitoring (TLM) versus those selected with conventional methods of selection in subfertile women undergoing ICSI. Methods The study population (239 women) was classified into two groups, based on the monitoring method used: Group 1 (TLM) and Group 2 (conventional monitoring). Groups were compared according to the clinical and ICSI cycle characteristics and reproductive outcomes, while transfers were performed at day 2 or 3. Subgroup analyses were performed, in women of both groups according to age and clinical parameters, and in embryos of Group 1 based on their cellular events.Results There was a statistically significant difference between the two study groups with regard to the outcome parameters, favoring Group 1 and especially in women >40 years of age. No differences were found in subgroup analyses in participants of both groups, regarding the stimulation protocol used, number of the oocytes retrieved and type of subfertility, while in Group 1 the percentages of "in range" cellular events were higher in certain divisions in ages 35-40, non-smokers, and the GnRH-agonist group, and in embryos that resulted in pregnancy. Conclusion Morphokinetic parameters of early embryo development via TLM are related to the characteristics of subfertile patients and associated with ICSI outcomes.

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“…Intra-observer variability evaluation demonstrated comparable results for most parameters. The authors indicated that embryo morphokinetic factors can be used certainly for embryo viability prediction, even the recording interpret by a trainee operator [54].…”

Section: Diferent Studies Based On Time-lapse Observationmentioning

confidence: 99%

Embryo Morphokinetics Based on Time-Lapse Observation

Tabibnejad¹

2017

Embryo Cleavage

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Embryo incubation and evaluation are critical steps in assisted reproductive technology (ART). Conventionally, embryo assessment has been done by embryologists through removing embryos from a conventional incubator during the culture period. Over recent years, time-lapse systems (TLS) have been established which can take digital images of embryos at key points and time intervals. This technique allows embryologists to assess the embryo quality in the steady culture environment. According to TLS studies and prepared algorithm models, it seems that TLS alone or in combination with conventional morphology can be considered as a useful diagnostic tool to determine high-quality embryos and improve embryonic implantation and pregnancy rates. In addition, there were remarkable diferences between embryo developmental time points and intervals regarding embryo gender, embryo fragmentation, and type of ovarian stimulation protocol. For conident conclusion, time-lapse imaging should be evaluated in further studies, and the system should be evaluated for cost/beneit ratio efectiveness in individual laboratory.Keywords: embryo cleavage, embryo morphokinetics, embryoscope, time-lapse imaging IntroductionAssisted reproductive technology (ART) may help infertile couples to realize their dream to have a child in their family, but pregnancy and live birth rates following in vitro fertilization (IVF) still remain low. It is ideal to identify viable embryos with the highest implantation potential to raise IVF success rates. In the traditional IVF practice, embryo assessments are mainly based on the morphologic observation and grade of embryologists at each stage of oocyte and embryonic development. Some features including oocyte and embryo quality, blastomere numbers and regularity, the percentage of fragmentation, and cytoplasmic granularity have been deined as prognostic indicators of successful pregnancy. This traditional embryo assessment method may have some detrimental efects on embryo growth because frequently opening and closing of incubators often cause to the change of embryo culture environmental steadiness. In order to reduce the inter-and intra-observer diference change, the time-lapse imaging (TLI) has been introduced into in vitro fertilization (IVF) laboratory. The application of time-lapse technology to the clinical IVF laboratory has supported more detailed observations on the embryo development researches quickly.The aim of this chapter is to determine whether TLI is useful for selection of "top quality" embryos for transfer to improve ART outcome rather than conventional morphological evaluation. The possible correlation between embryos' sex, embryo fragmentation, treatment protocols, diferent culture media, and embryo morphokinetics will be examined based on some new researches of TLI facilities. Furthermore, various algorithms and predictive models designed in ART cycles with TLI will be discussed.

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Inter- and intra-observer variability of time-lapse annotations

Abstract: NCT01139268.

Cited by 132 publications

References 19 publications

Does sperm origin affect embryo morphokinetic parameters?

Does sperm origin affect embryo morphokinetic parameters?

Morphokinetic parameters of early embryo development via time lapse monitoring and their effect on embryo selection and ICSI outcomes: a prospective cohort study

Embryo Morphokinetics Based on Time-Lapse Observation

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