Inter- and intra-rater reliability of computer-assisted planimetry in experimental stroke research

Braun, Tobias; Pukropski, Jan; Yeniguen, Mesut; El-Shazly, Jasmin; Schoenburg, Markus; Gerriets, Tibo; Kaps, Manfred; Tschernatsch, Marlene; Juenemann, Martin

doi:10.1016/j.jneumeth.2018.11.012

Cited by 1 publication

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“…The SWI sequences were checked for the presence of intracerebral haemorrhage. Computer-aided planimetric assessments of ischemic lesion volumes and hemispheric volumes were performed by two blinded investigators experienced in experimental stroke MRI and sufficient training in planimetry (27). After optimal adjustment of contrast, the edges of the hemispheres were traced manually on each slice using neuroanatomic landmarks.…”

Section: Neurological Examination and Imagingmentioning

confidence: 99%

Microbubble-mediated sonothrombolysis with BR38 of a venous full blood thrombus in a rat embolic stroke model

et al. 2021

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Background: Early recanalization of an occluded vessel is associated with a better clinical outcome in acute ischemic stroke. Intravenous thrombolysis using recombinant tissue plasminogen activator (rt-PA) is only available in a minority of patients and often fails to reopen the occluded vessel. Mechanical recanalization is more effective in this matter but only available for selected patients when a thrombectomy centre can be reached. Therefore, sonothrombolysis might represent an alternative or complementary approach. Here, we tested microbubble-mediated sonothrombolysis (mmSTL) in a thromboembolic stroke model for middle cerebral artery occlusion (MCAO) in rats.Methods: Sixty-seven male Wistar rats underwent MCAO using an autologous full blood thrombus and were randomly assigned to four groups receiving rt-PA, mmSTL, a combination of both, or a placebo. Diagnostic workup included neurological examination, assessment of infarct size, and presence of intracerebral haemorrhage by magnetic resonance imaging (MRI) and presence of microbleedings in histological staining.Results: Neurological examination revealed no differences between the treatment groups. In all treatment groups, there was a reduction in infarct size 24 hours after MCAO as compared to the placebo (P≤0.05), but there were no differences between the active treatment groups (P>0.05) (placebo 0.75±0.10 cm 3 ; mmSTL 0.43±0.07 cm 3 ; rt-PA 0.4±0.07 cm 3 ; mmSTL + rt-PA 0.27±0.08 cm 3 ). Histological staining displayed intracerebral microbleedings in all animals. The frequency of gross bleeding detected by MRI did not differ between the groups (placebo 3; mmSTL 4; rt-PA 2; mmSTL + rt-PA 2; P>0.05) and was not associated with worse performance in clinical testing (P>0.05). There were no statistical differences in the mortality between the groups (P>0.05).Conclusions: Our study showed the efficacy and safety of mmSTL with or without rt-PA in an embolic rat stroke model using a continuous full blood thrombus. Sonothrombolysis might be useful for patients who need to be transported to a thrombectomy centre or for those with distal vessel occlusion.

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Section: Neurological Examination and Imagingmentioning

confidence: 99%