Inter and intraspecific genetic diversity (RAPD) among three most frequent species of macrofungi ( Ganoderma lucidum , Leucoagricus sp. and Lentinus sp.) of Tropical forest of Central India

Abstract

In present study seven RAPD primers were used to access the diversity within and among twelve populations of three mushroom species Ganoderma lucidum, leucoagaricus sp. and Lentinus sp. Total of 111 bands were scored by 7 RAPD primers in 30 accessions of three mushroom species collected from different sampling sites of central India. Total 111 bands were generated using seven primers which were F-1, OPG-06, OPC-07, OPD-08, OPA-02, OPD-02, OPB-10. All 111 bands were polymorphic in nature (100%). Therefore, it revealed that the used primers had sufficient potency for population studies and 30 accessions had higher genetic differences among each other. In best of the knowledge, this is the first report, which accesses the genetic diversity between three mushroom species (Gd Ganoderma lucidum, Lg Leucoagaricus sp., Ls Lentinus). The polymorphic percentage ranged from 3.60 to 23% within twelve populations, while polymorphic percentage among group was 40.56, among population within groups was 41.12 and within population was 18.32. This indicated that the genetic diversity within the population was very low, but slightly higher in the populations of three species. Among three groups representing Gd., Lg and Ls, Among populations within groups shown highest percentage of variation (Pv = 41.12) while within populations, the lowest percentage of variation (18.32) was observed. This result also support that the highest genetic variation was present among groups in comparison to among the population within a species and lowest genetic variation was observed within the population.

Genotypic Characterization of Rhizopus species from Tempeh and Usar: Traditional Inoculum of Tempeh in Indonesia

“…Figure 4 showed that RAPD markers can show genetic variation in nine Rhizopus. Previously it has been reported that RAPD marker can be used as an important technique to investigate for the genetic variations of fungal (Dwivedi et al 2018). These reported are in line with our result, where RAPD marker can also distinguish the genetic variations of nine R. microsporus and two R. delemar well.…”

Improved production of polysaccharides in Ganoderma lingzhi mycelia by plasma mutagenesis and rapid screening of mutated strains through infrared spectroscopy

“…The RAPD assay is a common and useful method for the identification of fungal strains and for the identification of Ganoderma lucidum strains [ 45 , 46 ]. It is often used in mutagenesis identification as well [ 34 , 47 , 48 ].…”

Genetic diversity using random amplified polymorphic DNA (RAPD) and inter-simple sequence repeats (ISSR) markers in Tinospora cordifolia from the Rayalseema region in Andhra Pradesh

“…Though RAPD markers have the problem of reproducibility, until recently and till to date these are the proven markers in terms of cost effectiveness that were extensively used to study genetic diversity among the species of bacteria, fungi, insects, plants and animals. Genetic diversity of twelve populations of three mushroom species were evaluated using seven RAPD markers that were able to differentiate the percent variability within and among different populations of these mushroom species (Dwivedi et al, 2018). Sugarcane when genetic diversity was estimated contain similarity coefficient which ranged from 0.43 to 0.91 using thirty five RAPD markers while the ISSR primers could explain the similarity coefficient from 0.73 to 0.93 which is less compared to RAPD markers (Patel et al, 2018).…”