Inter-Individual and Inter-Strain Variations in Zebrafish Locomotor Ontogeny

Lange, Merlin; Neuzeret, Frederic; Fabrèges, Benoît; Froc, Cynthia; Bedu, Sébastien; Bally‐Cuif, Laure; Norton, William

doi:10.1371/journal.pone.0070172

Cited by 59 publications

(54 citation statements)

References 43 publications

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“…Some studies demonstrated that a large variability exists in the individual motion and shoaling tendency of the zebrafish according to their age or strain. For example, adults AB and casper zebrafish swim longer distance than ABstrg, EK, TU or WIK zebrafish [51]. Likewise, the interindividual distance between shoal members decreases from 16 body length to 3.5 body length between day 7 and five months after fertilization [52].…”

Section: Discussionmentioning

confidence: 99%

Strain differences in the collective behaviour of zebrafish (Danio rerio) in heterogeneous environment

Séguret¹,

Collignon²,

Halloy³

2016

R. Soc. open sci.

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Recent studies show differences in individual motion and shoaling tendency between strains of the same species. Here, we analyse collective motion and response to visual stimuli in two morphologically different strains (TL and AB) of zebrafish. For both strains, we observed 10 groups of 5 and 10 zebrafish swimming freely in a large experimental tank with two identical landmarks (cylinders or discs) for 1 h. We tracked the positions of the fish by an automated tracking method and compute several metrics at the group level. First, the probability of the presence shows that both strains avoid free space and are more likely to swim in the vicinity of the walls of the tank and the landmarks. Second, the analysis of landmarks occupancy shows that AB zebrafish are more present in their vicinity than TL ones and that both strains regularly transit from one to the other one with no preference on the long duration. Finally, TL zebrafish show a higher cohesion than AB zebrafish. Thus, environmental heterogeneity and duration of the trials allow to reveal individual and collective behavioural variabilities among different strains of zebrafish. These results provide a new insight into the need to take into account individual variability of zebrafish strains for studying collective behaviour.

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Section: Discussionmentioning

confidence: 99%

Strain differences in the collective behaviour of zebrafish (Danio rerio) in heterogeneous environment

Séguret¹,

Collignon²,

Halloy³

2016

R. Soc. open sci.

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“…Indeed, assessment of movement can be done in embryos and it was recently suggested as a potential assay for developmental neurotoxicity testing (Selderslaghs et al, 2010(Selderslaghs et al, , 2013. However, in many cases the patterns of responses as well as sensitivity to certain toxicants significantly differ between embryos and later stages (Airhart et al, 2007;Jin et al, 2009;Lange et al, 2012;Sloman and McNeil, 2012), and one particular disadvantage of embryonic stages is that spontaneous swimming activity is not yet established.…”

Section: Kes ''Locomotion Impairment'' and ''Reduction In Food Intake''mentioning

confidence: 99%

Development and application of the adverse outcome pathway framework for understanding and predicting chronic toxicity: II. A focus on growth impairment in fish

et al. 2015

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Adverse outcome pathways (AOPs) organize knowledge on the progression of toxicity through levels of biological organization. By determining the linkages between toxicity events at different levels, AOPs lay the foundation for mechanism-based alternative testing approaches to hazard assessment. Here, we focus on growth impairment in fish to illustrate the initial stages in the process of AOP development for chronic toxicity outcomes. Growth is an apical endpoint commonly assessed in chronic toxicity tests for which a replacement is desirable. Based on several criteria, we identified reduction in food intake to be a suitable key event for initiation of middle-out AOP development. To start exploring the upstream and downstream links of this key event, we developed three AOP case studies, for pyrethroids, selective serotonin reuptake inhibitors (SSRIs) and cadmium. Our analysis showed that the effect of pyrethroids and SSRIs on food intake is strongly linked to growth impairment, while cadmium causes a reduction in growth due to increased metabolic demands rather than changes in food intake. Locomotion impairment by pyrethroids is strongly linked to their effects on food intake and growth, while for SSRIs their direct influence on appetite may play a more important role. We further discuss which alternative tests could be used to inform on the predictive key events identified in the case studies. In conclusion, our work demonstrates how the AOP concept can be used in practice to assess critically the knowledge available for specific chronic toxicity cases and to identify existing knowledge gaps and potential alternative tests.

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“…These published estimates of U crit cover a range of values, from c . 5 to 28 L S s −1 , which might reflect differences in methodology or test temperature (Sfakianakis et al , ), genetic variation among test populations, many of which are sourced from local pet shops and have unpublished histories (Lange et al , ), age (Gilbert et al , ) or, as suggested by this study, sex. Few studies of U crit in D .…”

Section: Discussionmentioning

confidence: 78%

Sexually dimorphic morphology and swimming performance relationships in wild‐type zebrafish Danio rerio

Conradsen

McGuigan

2015

Journal of Fish Biology

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This study compared prolonged swimming performance (Ucrit ) between male and female Danio rerio, and characterized how body shape was associated with this performance measure in each sex. When swimming in small (n = 6) mixed-sex groups at 28 °C, males swam, on average, over 10 cm s(-1) faster than females despite being significantly smaller. Body shape was sexually dimorphic, with males and females exhibiting small, but statistically significant differences in most aspects of body shape. Body shape explained 18 and 43% of the variation in Ucrit among males and females. In general, effects of body shape on swimming performance appeared to be sex limited, whereby different aspects of body shape affected performance in each sex, although the contribution of the distance between pelvic and anal fins to swimming performance was weakly sexually antagonistic.

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Inter-Individual and Inter-Strain Variations in Zebrafish Locomotor Ontogeny

Cited by 59 publications

References 43 publications

Strain differences in the collective behaviour of zebrafish (Danio rerio) in heterogeneous environment

Strain differences in the collective behaviour of zebrafish (Danio rerio) in heterogeneous environment

Development and application of the adverse outcome pathway framework for understanding and predicting chronic toxicity: II. A focus on growth impairment in fish

Sexually dimorphic morphology and swimming performance relationships in wild‐type zebrafish Danio rerio

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