Inter simple sequence repeat (ISSR) to assess genetic diversity within a collection of wild lingonberry (Vaccinium vitis-idaea L.) clones

Abstract

Debnath, S. C. 2007. Inter simple sequence repeat (ISSR) to assess genetic diversity within a collection of wild lingonberry (Vaccinium vitis-idaea L.) clones. Can. J. Plant Sci. 87: 337-344. Forty-three wild lingonberry [Vaccinium vitis-idaea ssp. minus (Lodd) Hult.] clones collected from four Canadian provinces were assessed for genetic variability by using inter simple sequence repeat (ISSR). Fifteen primers generated 356 polymorphic ISSR-PCR bands. A substantial degree of genetic diversity was found among the wild collections. Cluster analysis by the unweighted pair-group method with arithmetic averages (UPGMA) separated the wild clones into four main clusters, and identified the two remaining clones as outliers. Furthermore, within four clusters, the genotypes tended to form sub-clusters that were in agreement with the principal coordinate (PCO) analysis. Geographical distribution explained 10% of total variation as revealed by analysis of molecular variance (AMOVA). The ISSR markers detected a sufficient degree of polymorphism to differentiate among lingonberry clones, making this technology valuable for germplasm management and the more efficient choice of parents in current lingonberry breeding programs. Les auteurs ont découvert un degré sensible de diversité génétique parmi les spécimens sauvages. L'analyse typologique par la méthode des paires-groupes non pondérés avec la moyenne arithmétique (UPGMA) a permis de séparer les clones en quatre groupes principaux et d'identifier les deux clones restants comme des aberrations. Par ailleurs, dans quatre groupes, les génotypes avaient tendance à former des sous-groupes concordant avec l'analyse de la coordonnée principale (PCO). La répartition géographique explique 10 % de la variation totale révélée par l'analyse de la variance moléculaire (AMOVA). Les marqueurs ISSR permettent de détecter un degré de polymorphisme suffisant pour qu'on différencie les clones d'airelle vigned'Ida, ce qui démontre l'utilité de cette technologie pour la gestion du matériel génétique et le choix de meilleurs parents pour les programmes actuels d'amélioration de cette culture.

EST-PCR, EST-SSR and ISSR markers to identify a set of wild cranberries and evaluate their relationships

“…The knowledge on optimal amplification condition and polymorphism level becomes useful data for cranberry researchers. While ISSRs were also found effective in diversity analysis of lowbush blueberry (Debnath 2009) and lingonberry (Debnath 2007b), EST-SSR and EST-PCR markers were used in blueberry (Boches et al 2006) and cranberry (Bell et al 2008), respectively. Zalapa et al (2014 and Bassil et al (2009) reported the use of SSR and EST-SSR markers, respectively, in discriminating cranberries.…”

Fingerprinting of Vaccinium corymbosum cultivars using DNA of fruits

“…The number of polymorphic bands (12.7 per primer in the two tissues) obtained with ISSR markers in V. corymbosum cultivars was slightly lower compared to other studies in other Vaccinium species [Debnath (2007) in V. vitis-idaea (23.7 polymorphic bands), Debnath (2009) in V. angustifolium (17 polymorphic bands) and Yakimowski and Eckert (2008) in V. stamineum (18 polymorphic bands)]. The differences in the number of polymorphic bands in these studies can be explained because different species were studied, although they all belong to the Vaccinium genus.…”

“…The high genetic diversity between V. corymbosum cultivars could be hypothetically explained by the high ploidy level (Boches et al 2006) and the origin of each cultivar that consequently makes the blueberry a highly heterozygote genetic structure. High levels of genetic variation were also observed with these molecular markers in V. myrtillus (Albert et al 2005), Vaccinium vitis-idaea (Debnath 2007) and V. angustifolium (Debnath 2009). …”

“…Their use is the most effective way for characterization and identification of species and cultivars with the advantage that they allow for a direct comparison of genetic material independently of environmental influences (Weising et al 1995;Debnath 2007). Moreover, it could be useful (i) to promote the efficient use of genetic variations in crop improvement programs, (ii) to help in germplasm characterization and (iii) field genebank management, and (iv) to guarantee proprietary-right protection (Paterson et al 1991;Debnath 2009).…”

“…So far only a few attempts have been made to identify and characterize blueberry cultivars by molecular markers and to determine genetic diversity in the highbush blueberry or other species of Vaccinium genus (Levi, Rowland 1997;Albert et al 2003;Garkava-Gustavsson et al 2005;Debnath 2007Debnath , 2009Yakimowski, Eckert 2008).…”