Inter-subject registration and application of the SIGMA rat brain atlas for regional labeling in functional ultrasound imaging

Vidal, Benjamin; Droguerre, Marine; Venet, Ludovic; Valdebenito, Marco; Mouthon, Franck; Zimmer, Luc; Charvériat, Mathieu

doi:10.1016/j.jneumeth.2021.109139

Cited by 4 publications

(5 citation statements)

References 15 publications

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“…Although we could obtain reliable CBV changes in tinnitus mice using baseline correction and group comparison, anesthesia may affect the brain activities during fUS examination. [ 44 ] Further experiments using awake mice could avoid the bias of anesthesia and provide stronger evidence. To carry out multi‐omics analyzing of the ACC, more than 50 mice were used to harvest ACC tissues.…”

Section: Discussionmentioning

confidence: 99%

Functional Connectivity Alterations and Molecular Characterization of the Anterior Cingulate Cortex in Tinnitus Pathology without Hearing Loss

Fan,

Guan,

Zhong

et al. 2023

Advanced Science

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Compared with individuals with hearing loss, tinnitus patients without hearing loss have more psychological or emotional problems. Tinnitus is closely associated to abnormal metabolism and function of the limbic system, a key brain region for emotion experience, but the underlying molecular mechanism remains unknown. Using whole‐brain microvasculature dynamics imaging, the anterior cingulate cortex (ACC) is identified as a key brain region of limbic system involve in the onset of salicylate‐induced tinnitus in mice. In the tinnitus group, there is enhanced purine metabolism, oxidative phosphorylation, and a distinct pattern of phosphorylation in glutamatergic synaptic pathway according to the metabolome profiles, quantitative proteomic, and phosphoproteomic data of mice ACC tissue. Electroencephalogram in tinnitus patients with normal hearing thresholds show that the functional connectivity between pregenual anterior cingulate cortex and the primary auditory cortex is significantly increased for high‐gamma frequency band, which is positively correlated with the serum glutamate level. These findings indicate that ACC plays an important role in the pathophysiology of tinnitus by interacting with the primary auditory cortex and provide potential molecular targets in the ACC for tinnitus treatment.

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Section: Discussionmentioning

confidence: 99%

Functional Connectivity Alterations and Molecular Characterization of the Anterior Cingulate Cortex in Tinnitus Pathology without Hearing Loss

Fan,

Guan,

Zhong

et al. 2023

Advanced Science

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“…Before data processing, all acquisitions were automatically registered together in Matlab (Vidal et al, 2021). Briefly, a custom template was obtained by computing the mean image of all acquisitions, generating a first mean reference image, before linear registration of each single initial acquisition on this reference image.…”

Section: Discussionmentioning

confidence: 99%

“…Pharmaco-fUS has already allowed to assess both the hemodynamic effects and functional connectivity of various drugs in rodents (Rabut et al, 2020; Vidal et al, 2020a; Vidal et al, 2021; Vidal et al, 2020b). In particular, a previous proof-of-concept study evaluated the pharmacological impact of donepezil (DPZ) on brain dynamics in wild-type mice (Vidal et al, 2020a).…”

Section: Introductionmentioning

confidence: 99%

Pharmaco-fUS in cognitive impairment: Lessons from a preclinical model

et al. 2022

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Background: There is an urgent need to understand and reverse cognitive impairment. The lack of appropriate animal models combined with the limited knowledge of pathophysiological mechanisms makes the development of new cognition-enhancing drugs complex. Scopolamine is a pharmacologic agent which impairs cognition and functional imaging in a wide range of animal species, similarly to what is seen in cognitive impairment in humans. Methods: In this study, using a functional ultrasound (fUS) neuroimaging technique, we monitored the impact of donepezil (DPZ), a potent acetylcholinesterase inhibitor and first-line treatment in patients with mild to moderate Alzheimer's disease, in a scopolamine-induced mouse model. Results: We demonstrated that despite its low impact on the cerebral blood volume (CBV) signal, scopolamine injection produced an overall decrease in functional connectivity between various brain areas. In addition, we revealed that DPZ induced a strong decrease in CBV signal without causing a difference in functional connectivity. Conclusion: Finally, our work highlighted that DPZ counteracted the impact of scopolamine on functional connectivity changes and confirmed the interest of using pharmaco-fUS imaging on cognitive disorders, both in frequent and rare neurological disorders.

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“…Before data processing, all acquisitions were automatically registered together in MATLAB as previously described ( Vidal et al, 2021 ), along with the data from the resting-state protocol that were acquired in the same plane. Briefly, a custom template was obtained by computing the mean image of all acquisitions, generating a first mean reference image, before linear registration of each single initial acquisition on this reference image.…”

Section: Methodsmentioning

confidence: 99%

Impaired Local and Long-Range Brain Connectivity and Visual Response in a Genetic Rat Model of Hyperactivity Revealed by Functional Ultrasound

et al. 2022

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Attention-Deficit hyperactivity disorder (ADHD) is a central nervous system (CNS) disorder frequently associated with other psychiatric disorders. Pathophysiology processes at stake in ADHD are still under investigation and interestingly neuroimaging data points to modulated brain connectivity in patients. The genetic spontaneously hypertensive rat (SHR) model has been widely used to study pathophysiological underpinnings of ADHD and resting-state brain connectivity using functional magnetic resonance imaging. Here, functional ultrasound imaging, a new technique enabling fast measurement of cerebral blood volume (CBV), was used to further characterize resting-state functional connectivity – at both local and long-range – and visual response in SHR. We demonstrated that response to visual stimulation was increased in SHR in the visual cortex and the superior colliculus. They displayed altered long-range functional connectivity between spatially distinct regions. SHR also displayed modulated local connectivity, with strong increases of regional homogeneity in parts of the motor and visual cortex, along with decreases in the secondary cingulate cortex, the superior colliculus and the pretectal area. As CBV is intricately coupled to cerebral activity, these results suggest an abnormal neural activity in the SHR animal model, consistent with previous clinical studies and demonstrate the potential of functional ultrasound imaging as a translational tool in ADHD.

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Inter-subject registration and application of the SIGMA rat brain atlas for regional labeling in functional ultrasound imaging

Cited by 4 publications

References 15 publications

Functional Connectivity Alterations and Molecular Characterization of the Anterior Cingulate Cortex in Tinnitus Pathology without Hearing Loss

Functional Connectivity Alterations and Molecular Characterization of the Anterior Cingulate Cortex in Tinnitus Pathology without Hearing Loss

Pharmaco-fUS in cognitive impairment: Lessons from a preclinical model

Impaired Local and Long-Range Brain Connectivity and Visual Response in a Genetic Rat Model of Hyperactivity Revealed by Functional Ultrasound

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