Interação enxerto e porta-enxerto na incidência da resinose do cajueiro

Cardoso, J. E.; Cavalcanti, J. J. V.; Cysne, Alex Queiroz; Sousa, Tomil Ricardo Maia de; Corrêa, Márcio Cléber de Medeiros

doi:10.1590/s0100-29452010005000101

Cited by 5 publications

(4 citation statements)

References 17 publications

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“…For the L. theobromae-inoculated plants, however, significant and continuous reductions in the F v /F m ratios over time demonstrated actual perturbations in plant metabolism, as clearly observed in both types of samples. For cashew plants, the pathogenic action of Lasiodiplodia is not fully understood, but damage to the plant might be occurring via reductions in water and nutrient transport, leading to reduced photosynthesis, which would explain the substantial decreases in the F v /F m ratios (Cardoso et al 2010). Previous determinations of the microscopic features of the colonization of cashew plants by L. theobromae (Muniz et al 2011) indicated the presence of the fungus within the xylem vessels.…”

Section: Discussionmentioning

confidence: 99%

“…Once infected, cashew seedlings constitute a primary inoculum for the fungus in the field and usually display no visual symptoms of infection. Therefore, the lack of an early detection method contributes to the spread of the disease (Cardoso et al 2010). Thus far, diagnosis and identification of the gummosis pathogen has been based upon the emergence of symptoms and subsequent isolation of the pathogen on agar media.…”

Section: Introductionmentioning

confidence: 99%

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Monitoring cashew seedlings during interactions with the fungus Lasiodiplodia theobromae using chlorophyll fluorescence imaging

Muniz¹,

Freire²,

Viana³

et al. 2014

Photosynt.

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The chlorophyll (Chl) fluorescence imaging technique was applied to cashew seedlings inoculated with the fungus Lasiodiplodia theobromae to assess any disturbances in the photosynthetic apparatus of the plants before the onset of visual symptoms. Two-month-old cashew plants were inoculated with mycelium of L. theobromae isolate Lt19 or Lt32. Dark-adapted and light-acclimated whole plants or previously labelled, single, mature leaf from each plant were evaluated weekly for Chl fluorescence parameters. From 21 to 28 days, inoculation with both isolates resulted in the significantly lower maximal photochemical quantum yield of PSII (F v /F m ) than those for control samples, decreasing from values of 0.78 to 0.62. In contrast, the time response of the measured fluorescence transient curve from dark-acclimated plants increased in both whole plants and single mature leaves in inoculated plants compared with controls. The F v /F m images clearly exhibited photosynthetic perturbations 14 days after inoculation before any visual symptoms appeared. Additionally, decays in the effective quantum yield of PSII photochemistry and photochemical quenching coefficient were also observed over time. However, nonphotochemical quenching increased during the evaluation period. We conclude that F v /F m images are the effective way of detecting early metabolic perturbations in the photosynthetic apparatus of cashew seedlings caused by gummosis in both whole plants and single leaves and could be potentially employed in larger-scale screening systems.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Monitoring cashew seedlings during interactions with the fungus Lasiodiplodia theobromae using chlorophyll fluorescence imaging

Muniz¹,

Freire²,

Viana³

et al. 2014

Photosynt.

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“…Ademais, diferentes combinações entre porta-enxertos e copa resultam em alterações fisiológicas das plantas, que podem originar melhores adaptações a diferentes condições ambientais (Paiva et al, 2008). Alguns autores evidenciam a existência de diferentes interações entre porta-enxertos e copas de cajueiro quanto à resistência às doenças (Cardoso et al, 2010), tolerância à salinidade (Carneiro et al, 2004;Ferreira-Silva et al, 2009;Sousa et al, 2011) e exigências hídricas (Oliveira et al, 2003). Ressaltese, ainda, a utilização de um número reduzido de genótipos, tanto como porta-enxerto quanto como copa, que caracteriza uma situação de vulnerabilidade genética a fatores bióticos ou abióticos que podem comprometer toda a cadeia produtiva (Melo Filho et al, 2006;Paiva et al, 2008 Os garfos utilizados foram obtidos dos jardins clonais de cajueiro localizados no CEP, que apresentavam aproximadamente 10 cm de comprimento e 5 mm de diâmetro, com gema terminal em início de brotação, conforme Cavalcanti Júnior (2005).…”

Section: Methodsunclassified

Porta-enxertos para a produção de mudas de cajueiro

Serrano

Melo

Taniguchi

et al. 2013

Pesq. agropec. bras.

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Resumo -O objetivo deste trabalho foi avaliar clones de cajueiro como porta-enxertos para quatro genótipos-copa. Foram avaliados como porta-enxertos os clones CCP 06, CCP 09, CCP 76, CCP 1001, Embrapa 50, Embrapa 51, BRS 189, BRS 226, BRS 253, BRS 265, BRS 274 e BRS 275 e, como copas, os clones CCP 76, BRS 189, BRS 226 e BRS 274. Aos 60 dias após a semeadura, os porta-enxertos foram avaliados quanto às características biométricas e quanto às taxas de germinação e de aptidão à enxertia. Aos 80 dias após a enxertia, foram avaliadas as taxas de sucesso da enxertia, a aptidão para o plantio e as características biométricas das mudas. Houve correlação negativa entre massa de semente e taxas de germinação e de aptidão à enxertia. BRS 226, BRS 253 e BRS 274 apresentaram taxas insatisfatórias de germinação e de aptidão à enxertia. As maiores taxas de plantas aptas ao plantio, para a copa CCP 76, ocorreram nos porta-enxertos CCP 06, CCP 76 e CCP 1001; para BRS 189, destacou-se o CCP 06; para BRS 226, destacaram-se CCP 06, Embrapa 50, Embrapa 51, BRS 189 e BRS 265; e, para BRS 274, os porta-enxertos CCP 06, CCP 76, CCP 1001, Embrapa 50, Embrapa 51 e BRS 189.Termos para indexação: Anacardium occidentale, enxertia, genótipo-copa, germinação de semente, matéria seca total. Rootstocks for the production of grafted cashew seedlingsAbstract -The objective of this work was to evaluate cashew clones as rootstocks for four scions. The clones CCP 06, CCP 09, CCP 76, CCP 1001, Embrapa 50, Embrapa 51, BRS 189, BRS 226, BRS 253, BRS 265, BRS 274, and BRS 275 were evaluated as rootstocks; and the clones CCP 76, BRS 189, BRS 226, and BRS 274 were evaluated as scions. At 60 days after sowing, the rootstocks were evaluated for their biometric characteristics, germination rate, and grafting ability. At 80 days after grafting, grafted seedlings were evaluated for their grafting success rates, planting ability, and biometric characteristics. Seed mass were negatively correlated with germination rate and grafting ability. BRS 226, BRS 253 and BRS 274 showed unsatisfactory germination rate and grafting ability. The highest planting ability rates for CCP 76 occurred with CCP 06, CCP 76, and CCP 1001 as rootstocks; for BRS 189, with CCP 06; for BRS 226, with CCP 06, Embrapa 50, Embrapa 51, BRS 189, and BRS 265; and, for BRS 274, with CCP 06, CCP 76, CCP 1001, Embrapa 50, Embrapa 51, and BRS 189.

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“…Initial symptoms appear as a wound in the trunk that evolves into a gum exudative canker and, soon after, the yellowing of the leaves and plant decay. Protective measures are not available, with treatments remaining inefficient (Cardoso et al, 2010). In addition to cashew plants, L. theobromae negatively impacts several different crop cultures, including avocado (Persea americana Mill.…”

Section: Introductionmentioning

confidence: 99%

Agrobacterium tumefaciens-mediated transformation of Lasiodiplodia theobromae, the causal agent of gummosis in cashew nut plants

Muniz¹,

Silva²,

Júnior³

et al. 2014

Genet. Mol. Res.

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ABSTRACT. Lasiodiplodia theobromae is a major pathogen of many different crop cultures, including cashew nut plants. This paper describes an efficient Agrobacterium tumefaciens-mediated transformation (ATMT) system for the successful delivery of T-DNA, transferring the genes of green fluorescent protein (gfp) and hygromycin B phosphotransferase (hph) to L. theobromae. When the fungal pycnidiospores were co-cultured with A. tumefaciens harboring the binary vector with hph-gfp gene, hygromycin-resistant fungus only developed with acetosyringone supplementation. The cashew plants inoculated with the fungus expressing GFP revealed characteristic pathogen colonization by epifluorescence microscopy. Intense and 2907 ATMT of Lasiodiplodia theobromae©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 13 (2): 2906-2913 (2014) bright green hyphae were observed for transformants in all extensions of mycelium cultures. The penetration of parenchyma cells near to the inoculation site, beneath the epicuticle surface, was observed prior to 25 dpi. Penetration was followed by the development of hyphae within invaded host cells. These findings provide a rapid and reproducible ATMT method for L. theobromae transformation.

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Interação enxerto e porta-enxerto na incidência da resinose do cajueiro

Cited by 5 publications

References 17 publications

Monitoring cashew seedlings during interactions with the fungus Lasiodiplodia theobromae using chlorophyll fluorescence imaging

Monitoring cashew seedlings during interactions with the fungus Lasiodiplodia theobromae using chlorophyll fluorescence imaging

Porta-enxertos para a produção de mudas de cajueiro

Agrobacterium tumefaciens-mediated transformation of Lasiodiplodia theobromae, the causal agent of gummosis in cashew nut plants

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