“…The UV/vis absorption spectra of tetracyclines are generally discussed as superposition of the contributions of two separated chromophores [22][23][24]: the first is the so-called A chromophore which comprises a tricarbonylmethane keto-enol system spanning from C1 to C3 including the substituents (O1, carboxamide group at C2 and OH3); the second, usually termed the BCD chromophore, comprises the π-electron system located on rings B, C and D (Fig. 3 a).…”