Interaction between cadmium and selenium in rat plasma

Abstract

The metabolism of 75Se-labeled SeO32-and its conversion by intact rat erythrocytes in vitro to a form which complexes with Cd and plasma proteins were studied. By utilizing both excess ethylmaleimide to lower erythrocyte reduced glutathione (GSH) concentrations, it was shown that the uptake and metabolism of SeO32-were GSH-dependent, the probable intermediate being glutathione selenotrisulfide (GSSeSG). Secondary release of selenium by rat erythrocytes had no relation to the erythrocyte transport of oxidized glutathione (GSSG). While fluoride depressed and chromate increased GSSG transport, chromate, a glutathione reductase inhibitor, decreased selenium release. This release appeared to be secondary to a reaction catalyzed by glutathione reductase. The similarity of 150 values for chromate's inhibition of glutathione reductase and for the inhibition of selenium release further suggested a relationship between these two events. H2Se or a similar product of GSSeSG reduction is proposed to be the active product of SeO32-metabolism by rat erythrocytes. By use of gel-filtration and ion-exchange methods it was noted that the incubation of H2Se with cadmium and plasma produced a Cd-Se complex indistinguishable from that produced by incubation of Cd, SeO32-, plasma, and erythrocytes in vitro, or that noted following the administration of Cd and SeO3 in vivo. A mechanism whereby the tissue distribution and toxicity of cadmium are altered by selenium is suggested.

Assessment of lifestyle effect on oxidative stress biomarkers in free‐living elderly in rural Japan

“…5 It has been documented that Cd can interact with Se in vivo; Cd toxicity can be prevented by Se, and exposure to a high dose of Cd suppresses anticancer effects of Se. 6,7 Because many of the acute and chronic age-related diseases have an underlying mechanism of oxidative stress, 8 assessment of oxidative stress in the elderly would be a worthwhile public-health goal.…”

The Interaction of Cadmium and Selenium in Horse Kidney Cortex in Relation to Histopathological Changes

“…The interactions of Cd with different cations, complexes, and biological compounds have been intensively studied (eg. Chen et al 1975, Piotrowski et al 1977, Gasiewicz & Smith 1978, Flegal et al 1980, Chavez 1981, Mahaffey et al 1981, Bozkurt & Smith 1981, Flora et al 1982, Tei/mann & Hansen 1984, Nielsen & Winge 1985. However, the information of the possible interaction of Cd and Se in kidneys is scanty.…”