2015
DOI: 10.1002/clen.201400006
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Interaction between Microbes DNA and Atrazine in Black Soil Analyzed by Spectroscopy

Abstract: It could be shown that atrazine bound to microbes DNA in black soil by using ethidium bromide (EB) as a fluorescence probe. To study the interaction between atrazine and the microbes DNA in black soil, spectral absorption and fluorescence analysis were adopted. Atrazine, when bound to DNA, showed a hyperchromic and red shift in the absorption spectra and fluorescence quenching (>50%) in the fluorescence spectra, which indicated the intercalation. The effect of phosphate ion showed that there were electrostatic… Show more

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Cited by 11 publications
(3 citation statements)
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“…After filtration, the samples were kept at −20 °C for the three-dimensional fluorescence spectral analysis. The organic carbon levels of the filtrate were detected using a total organic carbon (TOC) analyzer (TOC-VCPH, Shimadzu, Japan) [ 34 ]. Each measurement was carried out in triplicate.…”
Section: Methodsmentioning
confidence: 99%
“…After filtration, the samples were kept at −20 °C for the three-dimensional fluorescence spectral analysis. The organic carbon levels of the filtrate were detected using a total organic carbon (TOC) analyzer (TOC-VCPH, Shimadzu, Japan) [ 34 ]. Each measurement was carried out in triplicate.…”
Section: Methodsmentioning
confidence: 99%
“…However, in the process of constructing the evaporation layer with various photothermal materials, few people pay attention to the effect of the aggregation effect of the material itself on the transport of water and light utilization. The black soil (BS) formed under the condition of grassland vegetation in a temperate humid climate is rich in hydroxyl groups on the surface and has high surface energy and hydrophilicity. In addition, the soil aggregate structure of BS leads to self-organizing pores at the nanoscale, which in turn produces an ideal capillary effect for efficient water transport. In terms of photothermal performance, the special aggregate structure and abundant internal pores can realize multilevel utilization of sunlight and obtain excellent light absorption capacity . Therefore, as an excellent natural photothermal conversion material, BS can be successfully used in seawater desalination.…”
Section: Introductionmentioning
confidence: 99%
“…The USDA soil classification categorizes black land as Udoll black soil, which is also known as Mollisols [1]. There are just four large black soil regions in the world, one of which is located in Diversity 2020, 12, 476 2 of 15 the northeast plains of China [2]. Black land offers a vital soil resource, with soils that are highly productive fertile soil containing a high (5-8%) organic carbon content [3].…”
Section: Introductionmentioning
confidence: 99%