Interaction between mRNA, ribosomes and the cytoskeleton

Hesketh, John E.; Pryme, Ian F.

doi:10.1042/bj2770001

Cited by 200 publications

(109 citation statements)

References 80 publications

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“…This suggests that mechanisms unrelated to microtubule alterations bring about these responses. On the other hand, the cytoskeleton was suggested to be involved in the regulation of protein turnover (Hesketh and Pryme, 1991). Here, the interesting question arises as to what extent cytoskeletal alterations are involved in mediating the cell-volume effects on proteolysis and protein synthesis …”

Section: Methodsmentioning

confidence: 99%

Involvement of microtubules in the swelling-induced stimulation of transcellular taurocholate transport in perfused rat liver

Häussinger

Saha

Hallbrucker

et al. 1993

Biochemical Journal

101

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An increase of the hepatocellular hydratation state, induced by hypotonic exposure, amino acids or tauroursodeoxycholate, was shown to increase within minutes the Vmax of transcellular taurocholate transport and excretion into bile [Häussinger, Hallbrucker, Saha, Lang and Gerok (1992) Biochem. J. 288, 681-689]. This stimulatory effect of cell swelling on taurocholate excretion into bile is abolished in the presence of colchicine (5 microM). On the other hand, colchicine did not affect the stimulatory action of hypotonic cell swelling on 14CO2 production from [1-14C]glycine or [1-14C]glucose. Likewise, volume regulatory K+ fluxes following anisotonic exposure were not influenced in the presence of colchicine. Lumicolchicine (5 microM), a stereoisomer of colchicine without an inhibitory effect on microtubules, did not abolish the stimulation of taurocholate excretion into bile following hypo-osmotic exposure. Hypertonic cell shrinkage decreased taurocholate excretion into bile by about 35%; this effect was fully reversible upon normotonic re-exposure. With colchicine pretreatment, however, the hypertonicity-induced inhibition of taurocholate excretion was blunted and was no longer reversible upon normotonic re-exposure. The results suggest that stimulation of taurocholate excretion into bile in response to cell swelling involves a colchicine-sensitive, probably microtubule-dependent, mechanism, but not the stimulation of other cell-volume-sensitive pathways such as glycine oxidation or the pentose-phosphate shunt. It is hypothesized that the swelling-induced stimulation of taurocholate excretion into bile is due to a microtubule-dependent insertion of bile acid transporter molecules into the canalicular membrane.

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Section: Methodsmentioning

confidence: 99%

Involvement of microtubules in the swelling-induced stimulation of transcellular taurocholate transport in perfused rat liver

Häussinger

Saha

Hallbrucker

et al. 1993

Biochemical Journal

101

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“…The distance between the start and the final destination is 21 m. A video depicting this transport is available in the supplemental data online (http://www.plantcell.org). Bar ϭ 10 m. riched for translation factors, such as eEF1A (Lenk and Penman, 1979;Davies et al, 1991Davies et al, , 1996Hesketh and Pryme, 1991).…”

Section: Discussionmentioning

confidence: 99%

The Transport of Prolamine RNAs to Prolamine Protein Bodies in Living Rice Endosperm Cells[W]

et al. 2003

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RNAs that code for the major rice storage proteins are localized to specific subdomains of the cortical endoplasmic reticulum (ER) in developing endosperm. Prolamine RNAs are localized to the ER and delimit the prolamine intracisternal inclusion granules (PB-ER), whereas glutelin RNAs are targeted to the cisternal ER. To study the transport of prolamine RNAs to the surface of the prolamine protein bodies in living endosperm cells, we adapted a two-gene system consisting of green fluorescent protein (GFP) fused to the viral RNA binding protein MS2 and a hybrid prolamine RNA containing tandem MS2 RNA binding sites. Using laser scanning confocal microscopy, we show that the GFP-labeled prolamine RNAs are transported as particles that move at an average speed of 0.3 to 0.4 m/s. These prolamine RNA transport particles generally move unidirectionally in a stop-and-go manner, although nonlinear bidirectional, restricted, and nearly random movement patterns also were observed. Transport is dependent on intact microfilaments, because particle movement is inhibited rapidly by the actin filament-disrupting drugs cytochalasin D and latrunculin B. Direct evidence was obtained that these prolamine RNA-containing particles are transported to the prolamine protein bodies. The significance of these results with regard to protein synthesis in plants is discussed.

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“…Beside its participation in cytoplasm organization and cell locomotion actin filaments seem to play an important role in controlling gene expression. It is well documented that large population of mRNAs and polyribosomes are linked to the cytoskeleton [1]. At present it seems likely that untranslated mRNAs are associated with intermediate filaments [2,3], while most of the evidence suggests that cytoskeleton-bound polyribosomes are associated with the actin filaments [1,[4][5][6].…”

Section: Introductionmentioning

confidence: 99%

Alterations in protein synthesis induced by C2 toxin in 3T3 cells

Sklyarova¹,

Kostyukovski²,

Sharov³

et al. 1995

FEBS Letters

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We have studied the effect of actin skeleton depolymerisation induced by C2 toxin on protein synthesis in 3T3 cells. The toxin that was purified from culture medium of Clostridium botulinum type C was shown to specifically ADP-ribosylate actin in vitro and in vivo. Cells exposed to C2 toxin were rounded off, which was accompanied by disappearance of stress fibers. The rate of total protein synthesis decreased two-three times in the treated cells. This correlated with the reduction in amount of polyribosomes. The rates of specific protein synthesis were compared using 2D electrophoresis of pulse-labeled proteins. Dramatic changes were observed in the synthesis of a small group of cellular proteins. Our results indicate that actin filament depolymerization affects gene expression at the level of translation and/or through the control of mRNA concentrations.

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Interaction between mRNA, ribosomes and the cytoskeleton

Cited by 200 publications

References 80 publications

Involvement of microtubules in the swelling-induced stimulation of transcellular taurocholate transport in perfused rat liver

Involvement of microtubules in the swelling-induced stimulation of transcellular taurocholate transport in perfused rat liver

The Transport of Prolamine RNAs to Prolamine Protein Bodies in Living Rice Endosperm Cells[W]

Alterations in protein synthesis induced by C2 toxin in 3T3 cells

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