Interaction between nanoparticles generated by zinc chloride treatment and oxidative responses in rat liver

Abstract

The aim of the present study was to investigate the interaction of zinc chloride (3 mg/kg, intraperitoneally [ip]) in rat liver in terms of the biosynthesis of nanoparticles. Zinc treatment increased zinc content in rat liver. Analysis of fluorescence revealed the presence of red fluorescence in the liver following zinc treatment. Interestingly, the co-exposure to zinc (3 mg/kg, ip) and selenium (0.20 mg/L, per os [by mouth]) led to a higher intensity of red fluorescence compared to zinc-treated rats. In addition, X-ray diffraction measurements carried out on liver fractions of zinc-treated rats point to the biosynthesis of zinc sulfide and/or selenide nanocomplexes at nearly 51.60 nm in size. Moreover, co-exposure led to nanocomplexes of about 72.60 nm in size. The interaction of zinc with other mineral elements (S, Se) generates several nanocomplexes, such as ZnS and/or ZnSe. The nanocomplex ZnX could interact directly with enzyme activity or indirectly by the disruption of mineral elements’ bioavailability in cells. Subacute zinc or selenium treatment decreased malondialdehyde levels, indicating a drop in lipid peroxidation. In addition, antioxidant enzyme assays showed that treatment with zinc or co-treatment with zinc and selenium increased the activities of glutathione peroxidase, catalase, and superoxide dismutase. Consequently, zinc complexation with sulfur and/or selenium at nanoscale level could enhance antioxidative responses, which is correlated to the ratio of number of ZnX nanoparticles (X=sulfur or X=selenium) to malondialdehyde level in rat liver.

Toxicity of Glutathione-Binding Metals: A Review of Targets and Mechanisms

“…The metal-containing fraction of liver tissue, analyzed by X-ray diffraction, yielded reflection signals characteristic for cubic (zinc blende), zinc sulfide (ZnS), and/or zinc selenide (ZnSe) [ 166 ]. Co-administration of cadmium chloride and sodium selenite allows us to harvest the corresponding sulphide and selenide of cadmium both from the liver [ 167 ] and from the kidney [ 168 ]. The authors consider this phenomenon “ as a green biosynthesis of nanoparticles ”, and biological techniques that exploit plant cells and micro-organisms and for the biological production of metal sulphide nanoparticles are currently evaluated as an economically competitive and environmentally benign alternative to “chemical” processes [ 169 , 170 , 171 , 172 , 173 ].…”

The protection of Rhein lysinate to liver in diabetic mice induced by high-fat diet and streptozotocin

“…Fractions of tissues (50 mg) were homogenized in buffer (Tris 10 mM, EDTA 1 mM, phenylmethylsulfonyl fluoride (PMSF) 1 mM; pH = 7.40). The homogenates were centrifuged at 12,0009g for 20 min at 4°C to obtain supernatant fractions (Azzouz et al 2014). The antioxidant enzyme activities (including the activities of SOD and GSH-px) and the MDA content in the obtained supernatant were measured using assay kits, according to the manufacturer's instructions.…”

Effect of zinc and selenium on breast cancer risk: a NHANES cross-sectional study and mediation analysis

“…Also for selenium, in the form of selenoproteins (particularly selenocysteine), directly facilitates the reduction of H2O2 and other peroxides by catalysis [43]. The application of zinc or the simultaneous application of zinc and selenium resulted in elevated levels of glutathione peroxidase, catalase, and superoxide dismutase [44]. Therefore, the roles of zinc and selenium in mitigating oxidative stress and lipid peroxidation may have a positive impact on preventing or slowing the development of tumors.…”