INTERACTION BETWEEN PTERIDINE SYNTHESIS AND RIBOFLAVIN ACCUMULATION IN<i>DROSOPHILA MELANOGASTER</i>

Nickla, Harry

doi:10.1139/g72-013

Cited by 18 publications

(17 citation statements)

References 15 publications

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“…Riboflavin is an essential B vitamin in nearly all pro- and eukaryotic organisms, serving as a precursor for the synthesis of flavin coenzymes and flavin adenine dinucleotide which are essential cofactors for a wide variety of metabolic enzymes and electron transport38. In addition to metabolic processes, riboflavin is also involved in areas of yellow pigmentation in the mosquito, such as within the eyes and malpighian tubules39. The accumulation of riboflavin in mosquitoes exposed to live M. ulcerans , and the reduced accumulation in mosquitoes exposed dead M. ulcerans is surprising (Figure 5).…”

Section: Resultsmentioning

confidence: 99%

Analysis of the metabolome of Anopheles gambiae mosquito after exposure to Mycobacterium ulcerans

Hoxmeier

Thompson

Broeckling

et al. 2015

Sci Rep

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Infection with Mycobacterium ulcerans causes Buruli Ulcer, a neglected tropical disease. Mosquito vectors are suspected to participate in the transmission and environmental maintenance of the bacterium. However, mechanisms and consequences of mosquito contamination by M. ulcerans are not well understood. We evaluated the metabolome of the Anopheles gambiae mosquito to profile the metabolic changes associated with bacterial colonization. Contamination of mosquitoes with live M. ulcerans bacilli results in disruptions to lipid metabolic pathways of the mosquito, specifically the utilization of glycerolipid molecules, an affect that was not observed in mosquitoes exposed to dead M. ulcerans. These results are consistent with aberrations of lipid metabolism described in other mycobacterial infections, implying global host-pathogen interactions shared across diverse saprophytic and pathogenic mycobacterial species. This study implicates features of the bacterium, such as the putative M. ulcerans encoded phospholipase enzyme, which promote virulence, survival, and active adaptation in concert with mosquito development, and provides significant groundwork for enhanced studies of the vector-pathogen interactions using metabolomics profiling. Lastly, metabolic and survival data suggest an interaction which is unlikely to contribute to transmission of M. ulcerans by A. gambiae and more likely to contribute to persistence of M. ulcerans in waters cohabitated by both organisms.

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Section: Resultsmentioning

confidence: 99%

Analysis of the metabolome of Anopheles gambiae mosquito after exposure to Mycobacterium ulcerans

Hoxmeier

Thompson

Broeckling

et al. 2015

Sci Rep

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“…Here we describe a LRO in the Malpighian tubules of Drosophila that forms the major physiological zinc storage site in this animal. This zinc storage granule concentrates the entire chelatable pool of total body zinc in flies, and is distinct from the previously described riboflavin-containing granules that give the wild type tubule its characteristic yellow-orange color (NICKLA 1972).…”

Section: Introductionmentioning

confidence: 55%

“…Moreover, another common feature of all LRO-biogenesis mutants is a reduction of riboflavin containing pigment granules in their Malpighian tubules (BREHME and DEMEREC 1942;NICKLA 1972). Generally, yellow Malpighian tubules correlated with normal zinc content, whereas loss of coloration correlated with low body zinc ( Figure 4).…”

Section: Malpighian Tubule Lros Are a Major Site For Physiological Zimentioning

confidence: 98%

“…Furthermore, and despite the generally held idea that w mutants lack pigment because of defective transport of 3-hydroxy-kynurenine and 6-pyruvoyl tetrahydropterin into pigment granules (SULLIVAN et al 1979;EVANS et al 2008;GREEN et al 2012;HERSH 2016;NAVROTSKAYA and OXENKRUG 2016), earlier studies had demonstrated physical absence of these organelles in the w mutants (NOLTE 1961;SHOUP 1966;NICKLA 1972).…”

Section: Mapping the Mutant That Caused Three-fold Reduction In Totalmentioning

confidence: 98%

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Zinc storage granules in the Malpighian tubules ofDrosophila melanogaster

Tejeda-Guzmán

Rosas‐Arellano

Kröll

et al. 2017

Preprint

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Membrane transporters and sequestration mechanisms concentrate metal ions differentially into discrete subcellular microenvironments for usage in protein cofactors, signaling, storage, or excretion. Here we identify zinc storage granules as the insect’s major zinc reservoir in primary Malpighian tubule epithelial cells of Drosophila melanogaster. The concerted action of Adaptor Protein-3, Rab32, HOPS and BLOC complexes as well as of the white-scarlet (ABCG2-like) and ZnT35C transporters is required for zinc storage granule biogenesis. Due to similar lysosome related organelle defects, patients with Hermansky-Pudlak syndrome may lack zinc granules in beta pancreatic cells, intestinal paneth cells and presynaptic vesicles of hippocampal mossy fibers.

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“…The emergence of highthroughput technologies has given us possibilities to systematically survey and study the underlying biological system. The molecular interaction maps have been built in model organisms (e.g., S.cerevisiae [8], D.melanogaster [9] and C.elegans [10]), as well as in higher vertebrate organisms (e.g., zebrafish [11], mouse [12] and human [13]). Evidently, the generated interaction maps offer us a rich resource for systematic studies of molecular networks and complement other types of biological data.…”

Section: Introduction (Heading 1)mentioning

confidence: 99%

Network-based analysis of time series RNA-seq gene expression data by integrating the interactome and gene ontology information

Zhang

2014

2014 8th International Conference on Systems Biology (ISB)

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Monitoring the changes in gene expression patterns over time provides the distinct possibility of unraveling the mechanistic drivers characterizing cellular responses. Such time series gene expression data allow us to broadly "watch" the dynamics of the system. However, one challenge in the analysis of time series data is to establish and characterize the interplay between genes that are activated, deactivated or sustained in the context of a biological process or functional category. To address such challenges, novel algorithms are required to improve the interpretation of these data by integrating multi-source prior functional evidence. In this paper, we introduced a novel network-based approach to extract functional knowledge from time-dependent biological processes at a system level using time series mRNA deep sequencing data. First, a list of differentially expressed genes (DEGs) at each time point was identified. Second, GO terms that are enriched in each DEG list were identified. Third, the significance of interactions between DEGs in these GO terms at consecutive time points was measured. Finally, the significant interactions between DEGs in different GO terms were used to construct the interaction networks among GO terms between two consecutive time points, called GO networks. The proposed method was applied to investigate 1α, 25(OH)2D3altered mechanisms in zebrafish embryo development. GO networks were constructed over 4 consecutive time points. Results suggest that biological processes such as cartilage development and one-carbon compound metabolic process are temporally regulated by 1α,25(OH)2D3. Such discoveries could not have been identified with canonical gene set enrichment analyses. These results demonstrate that the proposed approach can provide insight on the molecular mechanisms taking place in vertebrate embryo development upon treatment with 1α,25(OH)2D3. Our approach enables the monitoring of biological processes that can serve as a basis for generating new testable hypotheses. Such network-based integration approach can be easily extended to any temporal-or condition-dependent genomic data analyses.

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INTERACTION BETWEEN PTERIDINE SYNTHESIS AND RIBOFLAVIN ACCUMULATION INDROSOPHILA MELANOGASTER

Cited by 18 publications

References 15 publications

Analysis of the metabolome of Anopheles gambiae mosquito after exposure to Mycobacterium ulcerans

Analysis of the metabolome of Anopheles gambiae mosquito after exposure to Mycobacterium ulcerans

Zinc storage granules in the Malpighian tubules ofDrosophila melanogaster

Network-based analysis of time series RNA-seq gene expression data by integrating the interactome and gene ontology information

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