Interaction Effect of Auxin and Cytokinin on &amp;lt;i&amp;gt;in Vitro&amp;lt;/i&amp;gt; Shoot Regeneration and Rooting of Endangered Medicinal Plant &amp;lt;i&amp;gt;Valeriana jatamansi&amp;lt;/i&amp;gt; Jones through Tissue Culture

Nazir, Urooj; Gul, Zishan; Shah, Ghulam Mujtaba; Khan, Nayyar Iqbal

doi:10.4236/ajps.2022.132014

Cited by 11 publications

(5 citation statements)

References 16 publications

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“…These observations align with previous studies in plant micropropagation that have reported IBA and NAA as suitable for root induction and optimal root length at low auxin concentration levels (Aroonpong and Chang 2015;Nazir et al 2022;Hejazi et al 2023).…”

Section: In Vitro Rootingsupporting

confidence: 91%

The effect of plant growth regulators on micropropagation of Melientha suavis Pierre. and assessment of genetic fidelity of regenerants based on iPBS and SRAP markers

SIRINGAM,

VANIJAJIVA

2023

Biodiversitas

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Abstract. Siringam T, Vanijajiva O. 2023. The effect of plant growth regulators on micropropagation of Melientha suavis Pierre. and assessment of genetic fidelity of regenerants based on iPBS and SRAP markers. Biodiversitas 24: 4628-4634. Melientha suavis Pierre, a significant deciduous edible plant species with high nutritional value and belonging to the Opiliaceae family, holds paramount importance for both agricultural and conservation purposes in its native Southeast Asia. However, the species faces challenges due to habitat fragmentation and negative anthropogenic impacts, leading to a decline in its population. This study aimed to explore the effects of various concentrations of plant growth regulators (cytokinin and auxin) on the shoot and root development of M. suavis, and to assess the genetic stability of micropropagated plants obtained from nodal explants. In vitro shoot regeneration and proliferation were conducted on Murashige and Skoog (MS) semi-solid medium, supplemented with 6-benzylaminopurine (BAP) or kinetin (Kn) at different doses. The optimal shoot length, shoot number, and leaf volume were observed in the modified MS medium with 1.0 mg/L BAP after an 8-week incubation period. Efforts to enhance the rooting of micropropagated shoots included the addition of auxins, such as ?-naphthalene acetic acid (NAA) or indole-3-butyric acid (IBA). However, the species exhibited recalcitrant behavior during the reproduction and rooting stages, as the rooting percentage did not correlate with the increase in auxin concentration. Interestingly, the highest root number and length were achieved in the MS medium without plant growth regulators after 8 weeks of incubation. To ensure genetic fidelity, regenerants were subjected to inter-primer binding site (iPBS) and sequence-related amplified polymorphism (SRAP) marker analysis. The results revealed no genetic variation between the micropropagated plants and the mother trees, confirming the production of genetically stable progeny. Overall, this protocol presents a promising alternative for profitable propagation and establishment of genetically constant progeny of M. suavis, with important implications for sustainable applications and germplasm conservation in Southeast Asia. Addressing the challenges faced by this valuable species through micropropagation and genetic fidelity assessment can contribute significantly to its preservation and utilization for agricultural and conservation initiatives.

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Section: In Vitro Rootingsupporting

confidence: 91%

The effect of plant growth regulators on micropropagation of Melientha suavis Pierre. and assessment of genetic fidelity of regenerants based on iPBS and SRAP markers

SIRINGAM,

VANIJAJIVA

2023

Biodiversitas

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“…These results are consistent with those of Sreekissoon et al (2021) who found that IBA was the most suitable auxin for root formation from nodal explants of Sceletium tortuosum. Auxin acts by attaching to a receptor protein and initiating a signaling cascade that most likely includes the proteolysis of transcription regulators (AUX/IAA) through the ubiquitin-proteasome system, which un-represses genes (Nazir et al, 2022). Furthermore, given that auxin has been demonstrated to play a key role in rooting apical organizing, it is conceivable to interpret the auxin oscillations from induction throughout formation as potentially connected to apical organizational processes (Nadal et al, 2022).…”

Section: Discussionmentioning

confidence: 99%

Enhancement Rutin Production from Capparis spinosa Plant by UV-C or Gamma Irradiation using In vitro Culture

Saleh,

M. Serag El-Din,

Youssef

2024

Egyptian Journal of Horticulture

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I N THE FRAMEWORK of climate changing adaptation and mitigation efforts, biotechnology can positively contribute to lowering the likelihood of environmental systems being affected by climate change's consequences. Caper bush is notoriously difficult to grow through traditional techniques, although despite the substantial social-economic and medical value of this species, its micropropagation has received very little study thus far. Therefore, in our study the internode segments were collected from the Southern of Sinai, and disinfected with various treatments of disinfectant agents and exposure times. Then the successive clean shootlets formed in the sterilization stage were separated and cultured on MS medium at various strengths of salts (Full, ¾, ½ and ¼ strength). The established micronodes were cut into small pieces, and re-cultured on MS medium supplemented with BAP or Kin each at 0.0, 1.0, 2.0 and 3.0 mg/l. The growing shootlets were removed individually and cultured on rooting MS medium supplemented with 0.0, 1.0, 2.0, 3.0 and 4.0 mg/l IBA or IAA for each, and finally the rooted explants were acclimatization in green house. UV-C rays exposure times (2, 4 and 6 hrs) or gamma radiation wave doses (0.5, 1.0 and 1.5 Gy) were applied. The successive rooted explants cultured on MS media containing IAA 4.0 mg/l (scored 41.66 % roots) were transferred on peat moss: vermiculite 2:1 which scored 41.66 % acclimatized plantlets. Also, gamma radiation at 1.0 Gy increased flavonoid and rutin content to 12.34 and 3.25 mg/g DW compared to 7.21 and 0.06 mg/g DW for control plants, respectively.

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“…The results of another study indicated that MS medium with 0.5 mgL -1 BAP and 0.25 mgL -1 IBA was the optimum medium for in vitro shoot multiplication of rodent tuber (16 shoots in 6 weeks) [27]. In the micropropagation of the medicinal plant Valeriana jatamansi Jones utilizing rhizome explants, it was discovered that adding BAP up to 1 -2 mgL -1 increased shoot length, number of leaves, and leaf growth when used in combination with NAA at 0-1 mgL -1 [28]. This is consistent with research on the P14 treatment (50 ppm BAP and 25 ppm NAA), which produced 8.10 shoots.…”

Section: The Effect Of Pgr Bap and Naa Combination On Rodent Tuber Sh...mentioning

confidence: 99%

Ex vitro shoot induction of rodent tuber (Typhonium flagelliforme) with benzyl amino purine and naphthalene acetic acid

Hardiany,

Fauzan,

Pramono

et al. 2023

IOP Conf. Ser.: Earth Environ. Sci.

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A medicinal plant called rodent tuber has the potential to both prevent and treat various diseases. There is no Standard Operating Procedure (SOP) governing the growth method of rodent tuber because it still grows wild and is rarely cultivated. This research aimed to study the optimal growth of rodent tuber for mass seed propagation based on the type of explants and the concentration of auxin and cytokinin combinations for inducing shoots. While generative parameters (harvesting of tubers/shoots) were observed up to 13 WAP (Week After Planting), vegetative parameters were observed up to 10 WAP. The results showed that the best type of explants for shoot induction and growth of rodent tuber were whole plants with a survival rate of 100%; the number of shoots was 6.65; the plant height was 21.27 cm with 6.34 leaves; 26.56 roots with a length of 16.05 cm; and 40.80% flowering plants. PGR (Plant Growth Regulator) combination of 50 ppm BAP (Benzyl Amino Purine) and 100 ppm NAA (Naphthalene Acetic Acid) became the best explant soaking solution by forming eight shoots and the height of plants was 24.13 cm with seven leaves. The explant produced the highest root length in a combination of 100 ppm BAP and 50 ppm NAA, which was 17.70 cm. The interaction between whole plant explants immersed in 0 ppm BAP and 100 ppm NAA solution resulted in the most significant number of roots (41). Those goals can support the SOP of propagated methods and ensure the consistency, quality, and availability of raw materials for traditional medicine.

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Interaction Effect of Auxin and Cytokinin on <i>in Vitro</i> Shoot Regeneration and Rooting of Endangered Medicinal Plant <i>Valeriana jatamansi</i> Jones through Tissue Culture

Cited by 11 publications

References 16 publications

The effect of plant growth regulators on micropropagation of Melientha suavis Pierre. and assessment of genetic fidelity of regenerants based on iPBS and SRAP markers

The effect of plant growth regulators on micropropagation of Melientha suavis Pierre. and assessment of genetic fidelity of regenerants based on iPBS and SRAP markers

Enhancement Rutin Production from Capparis spinosa Plant by UV-C or Gamma Irradiation using In vitro Culture

Ex vitro shoot induction of rodent tuber (Typhonium flagelliforme) with benzyl amino purine and naphthalene acetic acid

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