Interaction of a plant pseudo-response regulator with a calmodulin-like protein

Perochon, Alexandre; Dieterle, Stefan; Pouzet, Cécile; Aldon, Didier; Galaud, Jean‐Philippe; Ranty, Benoı̂t

doi:10.1016/j.bbrc.2010.07.016

Cited by 38 publications

(39 citation statements)

References 24 publications

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“…These data indicate a link between the functional deficiency of the DDB1 signal transduction gene in the hp1 mutant and APRR2-Like gene expression. Evidence from Arabidopsis indicates that the APRR2 gene product interacts with the calmodulin-like protein CLM9, which is involved in tolerance to abiotic stress and the regulation of seed germination by ABA (Perochon et al, 2010). Additionally, APRR1, a gene related to APRR2, has been reported to encode a protein that interacts with the ABSCISIC ACID INSENSITIVE3 (ABI3) transcription factor (Kurup et al, 2000).…”

Section: Ripening-related Gene Expression Is Altered In Tomatomentioning

confidence: 99%

Network Inference Analysis Identifies an APRR2-Like Gene Linked to Pigment Accumulation in Tomato and Pepper Fruits

et al. 2013

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Carotenoids represent some of the most important secondary metabolites in the human diet, and tomato (Solanum lycopersicum) is a rich source of these health-promoting compounds. In this work, a novel and fruit-related regulator of pigment accumulation in tomato has been identified by artificial neural network inference analysis and its function validated in transgenic plants. A tomato fruit gene regulatory network was generated using artificial neural network inference analysis and transcription factor gene expression profiles derived from fruits sampled at various points during development and ripening. One of the transcription factor gene expression profiles with a sequence related to an Arabidopsis (Arabidopsis thaliana) ARABIDOPSIS PSEUDO RESPONSE REGULATOR2-LIKE gene (APRR2-Like) was up-regulated at the breaker stage in wild-type tomato fruits and, when overexpressed in transgenic lines, increased plastid number, area, and pigment content, enhancing the levels of chlorophyll in immature unripe fruits and carotenoids in red ripe fruits. Analysis of the transcriptome of transgenic lines overexpressing the tomato APPR2-Like gene revealed up-regulation of several ripening-related genes in the overexpression lines, providing a link between the expression of this tomato gene and the ripening process. A putative ortholog of the tomato APPR2-Like gene in sweet pepper (Capsicum annuum) was associated with pigment accumulation in fruit tissues. We conclude that the function of this gene is conserved across taxa and that it encodes a protein that has an important role in ripening.

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Section: Ripening-related Gene Expression Is Altered In Tomatomentioning

confidence: 99%

Network Inference Analysis Identifies an APRR2-Like Gene Linked to Pigment Accumulation in Tomato and Pepper Fruits

et al. 2013

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“…1 Experimental evidences demonstrate the ability of CML9 to bind Ca 2+ ions 11 and CML9 was shown to fulfil under certain conditions the role of CaM in yeast. 12 More recently, Perochon et al 13,14 and Popescu et al 15 identified CML9-interacting proteins suggesting that CML9 participates in Ca 2+ -regulated processes in plant. It was reported by various approaches (two-hybrid screens, in vitro or in planta interactions) that several transcription factors could be the downstream targets of CML9.…”

Section: What Could Be the Relationship Between Cml9 And The Flagellimentioning

confidence: 99%

CML9, a multifunctionalArabidopsis thalianacalmodulin-like protein involved in stress responses and plant growth?

Leba

Perochon²,

Cheval

et al. 2012

Plant Signaling & Behavior

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Many stimuli such as hormones and stress factors elicit changes in intracellular calcium content that serve to convey information and activate appropriate responses. 1 These Ca 2+ signals are perceived by different Ca 2+ sensors, and calmodulin (CaM) is one of the best characterized Ca 2+ sensors in eukaryotes. Calmodulinlike (CML) proteins extend the Ca 2+ -toolkit in plants; CMLs share sequence similarity with the ubiquitous and highly conserved CaM, however, except for some of them, their roles at physiological and molecular levels remain largely unknown. 2,3In our group, we reported data on Arabidopsis thaliana CML9 that exhibits 46% amino acid sequence identity with CaM. 4,5 We showed that CML9 transcripts are found in all organs and that CML9 gene is rapidly induced by both abiotic and biotic stress. In a recent publication in Plant Journal, we demonstrated that CML9 expression is also rapidly induced by the phytopathogenic bacteria Pseudomonas syringae pv tomato DC3000 (Pst DC3000) 4 and that this upregulation belongs to salicylic acid (SA) production and to the flagellin perception receptor FLS2. Moreover, exogenous applications of SA or flg22, the biological active peptide of flagellin, are also able to induce rapid and transient CML9 gene expression and using a reverse genetic approach, we established that CML9 participates in plant innate immunity through a flagellin-dependent signaling pathway. 4 In addition to mediate plant innate immunity, flagellin and flg22 are also known to inhibit root elongation and seedling growth.6 Thus, we explored this facet of flagellin effect and we bring here new informations on plant growth behavior of CML9 overexpressing and knockout lines upon flg22 treatments and Plants have evolved complex signaling networks to respond to their fluctuating environment and adapt their growth and development. Calcium-dependent signaling pathways play key role in the onset of these adaptive responses. in plant cells, the intracellular calcium transients are triggered by numerous stimuli and it is supposed that the large repertory of calcium sensors present in higher plants could contribute to integrate these signals in physiological responses. here, we present data on CmL9, a calmodulin-like protein that appears to be involved in plant responses to both biotic and abiotic stress. using a reverse genetic approach based on gain and loss of function mutants, we present here data indicating that this CmL might also be involved in root growth control in response to the flagellin, a pathogen-associated molecular pattern (PamP) also involved in plant immunity. Keywords: Calmodulin-like protein, Arabidopsis thaliana, root growth, flagellin, gibberellins discuss the possible involvement of CML9 in plant growth control through hormonal compounds. CML9, a multifunctional The cml9 Genotypes Exhibit Altered Responses to FlagellinAs previously showed, cml9 mutants or CML9 overexpressors exhibit respectively an enhanced susceptibility and a better resistance against phytopathogenic bacteria. 4 Using...

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“…They were then recombined into bait and prey vectors derived from pGADT7 and pGBKT7 plasmids (Clontech). Analysis of protein-protein interactions was performed using the Gal4 two-hybrid assay as described previously (Perochon et al, 2010). 3-AT was included as a competitive inhibitor of the imidazoleglycerolphosphate dehydratase protein to increase the stringency and prevent autoactivation.…”

Section: Yeast Two-hybrid Analysismentioning

confidence: 99%

TaFROG encodes a Pooideae orphan protein that interacts with SnRK1 and enhances resistance to the mycotoxigenic fungus Fusarium graminearum

et al. 2015

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Interaction of a plant pseudo-response regulator with a calmodulin-like protein

Cited by 38 publications

References 24 publications

Network Inference Analysis Identifies an APRR2-Like Gene Linked to Pigment Accumulation in Tomato and Pepper Fruits

Network Inference Analysis Identifies an APRR2-Like Gene Linked to Pigment Accumulation in Tomato and Pepper Fruits

CML9, a multifunctionalArabidopsis thalianacalmodulin-like protein involved in stress responses and plant growth?

TaFROG encodes a Pooideae orphan protein that interacts with SnRK1 and enhances resistance to the mycotoxigenic fungus Fusarium graminearum

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