Interaction of Aeromonas Strains with Lactic Acid Bacteria via Caco-2 Cells

Abstract

The genus Aeromonas includes some species that have now been identified as human pathogens of significant medical importance. We investigated the ability of 13 selected Aeromonas strains belonging to nine species isolated from clinical cases (n ‫؍‬ 5), environmental waters (n ‫؍‬ 5), and fish (n ‫؍‬ 3) to adhere to and translocate Caco-2 cells in the absence and presence of two lactic acid bacteria (LAB), i.e., Lactobacillus acidophilus and Bifidobacterium breve. Aeromonas isolates were also assessed for their cytotoxicity, the presence of virulence genes, and hemolysin production. Among the clinical isolates, one strain of Aeromonas veronii biovar veronii and two strains of Aeromonas hydrophila carried cytotoxin (act), heat-labile toxin (alt), hemolysin (hlyA), and aerolysin (aerA) genes, were cytotoxic to Vero cells, produced hemolysin, and showed higher adherence to Caco-2 cells. In contrast, this was seen in only one environmental strain, a strain of A. veronii biovar sobria. When Aeromonas strains were coinoculated with LAB onto Caco-2 cells, their level of adhesion was reduced. However, their rate of translocation in the presence of LAB increased and was significantly (P < 0.05) higher among fish strains. We suggest that either the interaction between Aeromonas and LAB strains could have a detrimental effect on the Caco-2 cells, allowing the Aeromonas to translocate more readily, or the presence of the LAB stimulated the Aeromonas strains to produce more toxins and/or increase their translocation rate.

Efficacy of Selected Live Biotherapeutic Candidates to Inhibit the Interaction of an Adhesive-Invasive Escherichia coli Strain with Caco-2, HT29-MTX Cells and Their Co-Culture

“…The adhesion of AIEC alone, LBPs strains alone, and AIEC in the presence of the LBP strains was tested on both cell lines and their co-culture using methods described previously [ 35 ], with modifications from previous protocols [ 36 , 37 ]. Before the adhesion assay, cell lines were seeded onto an eight-well glass chamber slide system (Nunc Lab-Tek II).…”

Isolation and characterization of Enterobacteriaceae species infesting post-harvest strawberries and their biological control using bacteriophages

“…Following the high-resolution biochemical fingerprinting method (PhPlate system), representative isolates from different clonal clusters were selected and tested for their adhesion to and translocation through Caco-2 cells (ATCC = HTB37) (Grey and Kirov 1993;Hatje et al 2014). The adherence index used for the number of bacterial cells adhering to the Caco-2 cells was as follows: low adherence: 1-10; adherent: >10-20; high adherence: >20 (Wells et al 1999;Snowden et al 2006).…”

“…In this assay, the selected bacterial strains and phages were simultaneously inoculated onto the Caco-2 cell line and their adhesion was measured after 1.5 h. In the exclusion adhesion assay, polyvalent phage suspension was inoculated onto Caco-2 cells 45 min prior to inoculation of the enteric isolates (10 8 CFU/well). The bacterial isolates were then inoculated onto the cells and their adhesion was measured after 1.5 h. Translocation of strains selected through Caco-2 cells was tested as described before by Hatje et al (2014). Fig.…”

Antibacterial Properties of Flavonoids from Kino of the Eucalypt Tree, Corymbia torelliana

“…No cytopathic effect (CPE), such as detachment or rounding of the cells, was observed. However, cells showed morphological changes, characterized by shrinking, within 48 h. Studies investigating the CPE of bacterial toxins on eukaryotic cells, including Vero cells, have defined cytotoxicity as 50% or more of the cells showing CPE such as rounding or disruption of the cell monolayer within 4 h [ 38 , 39 , 40 ].…”