Interaction of bacteria-feeding soil flagellates and Pseudomonas spp.

Pedersen, Annette L.; Johansen, Anders; Winding, Anne

doi:10.1007/s00374-009-0417-4

Cited by 9 publications

(12 citation statements)

References 35 publications

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“…To examine further as to how differences in the mode of action of Pseudomonas secondary metabolites relate to their effect on protozoa, Pedersen et al (2010) incubated the protozoan C. longicauda in batch cultures with three different P. fluorescens strains that we also used in the experiments reported here. These three P. fluorescens strains have contrasting secondary metabolite properties.…”

Section: Discussionmentioning

confidence: 99%

“…Please notice that a treatment without bacteria (no bacteria added) was included as a negative control. Jousset et al, 2006;Pedersen et al, 2010). Further, growth of different protozoa increased considerably when grown on mutants where synthesis of secondary metabolites was blocked completely compared with wild-type bacteria, which produce the secondary metabolites (Jousset et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

“…Thus, the type strain DSM50090 T produces no known secondary metabolites, DR54 produces a membrane-bound cyclic lipopeptide, and CHA0 produces various extracellular metabolites. For all three Pseudomonas strains, Pedersen et al (2010) set up batch cultures with washed bacterial suspensions, presumed to be devoid of extracellular metabolites, as well as unwashed cultures retaining potential extracellular metabolites. In accordance with their assumptions, Pedersen et al (2010) found that when offered washed CHA0, C. longicauda was able to multiply, whereas for the two other Pseudomonas strains washed and unwashed bacteria affected C. longicauda similarly.…”

Section: Discussionmentioning

confidence: 99%

“…For all three Pseudomonas strains, Pedersen et al (2010) set up batch cultures with washed bacterial suspensions, presumed to be devoid of extracellular metabolites, as well as unwashed cultures retaining potential extracellular metabolites. In accordance with their assumptions, Pedersen et al (2010) found that when offered washed CHA0, C. longicauda was able to multiply, whereas for the two other Pseudomonas strains washed and unwashed bacteria affected C. longicauda similarly. Likewise, Andersen & Winding (2004) found that cell extract from P. fluorescens DR54 inhibited a mixed community of soil protozoa.…”

Section: Discussionmentioning

confidence: 99%

“…Thus, we hope to find protozoan characteristics that correlate with their ability to grow on specific bacteria. Moreover, we hypothesize that Pseudomonas production of secondary metabolites will affect protozoan growth compared with non‐metabolite producers, and, that this effect may be related to the nature of the toxin (Pedersen et al , 2010).…”

Section: Introductionmentioning

confidence: 99%

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Protozoan growth rates on secondary-metabolite-producing Pseudomonas spp. correlate with high-level protozoan taxonomy

Pedersen

Winding²,

Altenburger

2011

FEMS Microbiology Letters

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Different features can protect bacteria against protozoan grazing, for example large size, rapid movement, and production of secondary metabolites. Most papers dealing with these matters focus on bacteria. Here, we describe protozoan features that affect their ability to grow on secondary-metabolite-producing bacteria, and examine whether different bacterial secondary metabolites affect protozoa similarly. We investigated the growth of nine different soil protozoa on six different Pseudomonas strains, including the four secondary-metabolite-producing Pseudomonas fluorescens DR54 and CHA0, Pseudomonas chlororaphis MA342 and Pseudomonas sp. DSS73, as well as the two nonproducers P. fluorescens DSM50090(T) and P. chlororaphis ATCC43928. Secondary metabolite producers affected protozoan growth differently. In particular, bacteria with extracellular secondary metabolites seemed more inhibiting than bacteria with membrane-bound metabolites. Interestingly, protozoan response seemed to correlate with high-level protozoan taxonomy, and amoeboid taxa tolerated a broader range of Pseudomonas strains than did the non-amoeboid taxa. This stresses the importance of studying both protozoan and bacterial characteristics in order to understand bacterial defence mechanisms and potentially improve survival of bacteria introduced into the environment, for example for biocontrol purposes.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Protozoan growth rates on secondary-metabolite-producing Pseudomonas spp. correlate with high-level protozoan taxonomy

Pedersen

Winding²,

Altenburger

2011

FEMS Microbiology Letters

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Soil protists: An untapped microbial resource of agriculture and environmental importance

Chandarana

Amaresan

2022

Pedosphere

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Soil bacteria and protists show different sensitivity to polycyclic aromatic hydrocarbons at controlled chemical activity

Winding

Modrzyński

Christensen

et al. 2019

FEMS Microbiology Letters

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This study linked growth inhibition of soil bacteria and protists to the chemical activity (a) of polycyclic aromatic hydrocarbons (PAHs) and compared the sensitivities of bacteria and protists. Passive dosing from pre-loaded silicone provided well-defined and constant a of PAHs in independent tests. Single-species growth inhibition with two bacterial (Pseuodomonas fluorescens DR54 and Sinorhizobium meliloti) and two protist (Cercomonas longicauda and Acanthamoeba castellanii) strains at maximum a (amax) of nine and four PAHs, respectively, showed no inhibition of PAHs with amax below 0.1 (pyrene and anthracene), while growth inhibition was observed for PAHs with amax above 0.1 (e.g. fluorene, fluoranthene and naphthalene). The bacteria were less sensitive than the protists. Soil bacterial community-level growth inhibition by naphthalene was in good agreement with single-species data, but also indicated the presence of sensitive bacteria that were inhibited by a below 0.05 and increasing pre-exposure time giving higher inhibition. The a of 50% inhibition (Ea50) was 0.434 and 0.329 for 0.5 and 4 h pre-exposure time, respectively. Invertebrates tended to be more sensitive than single-celled organisms tested here. This suggests that PAH exposure leads to differential toxicity in soil biota, which may affect soil food web structure and cycling of organic matter.

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Interaction of bacteria-feeding soil flagellates and Pseudomonas spp.

Cited by 9 publications

References 35 publications

Protozoan growth rates on secondary-metabolite-producing Pseudomonas spp. correlate with high-level protozoan taxonomy

Protozoan growth rates on secondary-metabolite-producing Pseudomonas spp. correlate with high-level protozoan taxonomy

Soil protists: An untapped microbial resource of agriculture and environmental importance

Soil bacteria and protists show different sensitivity to polycyclic aromatic hydrocarbons at controlled chemical activity

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