Interaction of basic polyelectrolytes with the red blood cell II. Agglutination of red blood cells by polymeric bases

Karchalsky, A.; Danon, D.; Nevo, Abraham C.; Vries, A. De

doi:10.1016/0006-3002(59)90505-0

Cited by 197 publications

(58 citation statements)

References 16 publications

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“…Such synthetic vectors often show poor transfection efficiency coupled with transient gene expression [3,4]. They can display an unfavourable pharmacokinetic profile with high levels of liver/lung capture [5,6] and many have unacceptably toxicity [4,7,8]. Moreover most polyplexes are ultimately impractical to manufacture on an industrial scale and/or use clinically.…”

Section: Introductionmentioning

confidence: 99%

Intracellular fate of bioresponsive poly(amidoamine)s in vitro and in vivo

Richardson

Pattrick

Lavignac

et al. 2010

Journal of Controlled Release

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Linear poly(amidoamine)s (PAAs) have been designed to exhibit minimal non-specific toxicity, display pHdependent membrane lysis and deliver genes and toxins in vitro. The aim of this study was to measure PAA cellular uptake using ISA1-OG (and as a reference ISA23-OG) in B16F10 cells in vitro and, by subcellular fractionation, quantitate intracellular trafficking of 125 I-labelled ISA1-tyr in liver cells after intravenous (i.v.) administration to rats. The effect of time after administration (0.5-3 h) and ISA1 dose (0.04-100 mg/kg) on trafficking, and vesicle permeabilisation (N-acetyl-b-D-glucosaminidase (NAG) release from an isolated vesicular fraction) were also studied. ISA1-OG displayed~60-fold greater B16F10 cell uptake than ISA23-OG. Passage of ISA1 along the liver cell endocytic pathway caused a transient decrease in vesicle buoyant density (also visible by TEM). Increasing ISA1 dose from 10 mg/kg to 100 mg/kg increased both radioactivity and NAG levels in the cytosolic fraction (5-10 fold) at 1 h. Moreover, internalised ISA1 provoked NAG release from an isolated vesicular fraction in a dose-dependent manner. These results provide direct evidence, for the first time, of PAA permeabilisation of endocytic vesicular membranes in vivo, and they have important implications for potential efficacy/toxicity of such polymeric vectors.

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Section: Introductionmentioning

confidence: 99%

Intracellular fate of bioresponsive poly(amidoamine)s in vitro and in vivo

Richardson

Pattrick

Lavignac

et al. 2010

Journal of Controlled Release

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“…During these experiments, it became clear that sweat and saliva from patients lost transport inhibitory activity when stored in glass containers. Since it is known that glass interacts electrostatically with strongly positively charged polyamino acids and removes them from their solutions [5], we investigated the possibility that the sodium transport inhibitory factor of CFP may be a positively charged macromolecular substance.…”

Section: Introductionmentioning

confidence: 99%

“…Polylysine decreases the virulence of tobacco mosaic virus [2], agglutinates bacteria [6] and chicken erythrocytes [14], and agglutinates and hemolyzes human erythrocytes [5,13]. Protamine and polyethylene imine affect viability and decrease the size of experimental tumors [1,12].…”

Section: Introductionmentioning

confidence: 99%

Studies on the Mechanism of Inhibition of Sodium Transport in Cystic Fibrosis of the Pancreas

Mangos

McSherry

1968

Pediatr Res

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“…The surfaces of the cells are negatively charged, whereas poly-L-lysine is a cationic polymer. Thus, it is likely that poly-L-lysine bridged all the blood cells subsequently precipitating all of them (27).…”

Section: Discussionmentioning

confidence: 99%

Platelet Separation From Whole Blood in an Aqueous Two-Phase System With Water-Soluble Polymers

et al. 2006

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Abstract. Platelet-rich plasma (PRP) stimulates tissue healing and centrifugation is the only method for PRP preparation. The purpose of the present study was to develop a method to separate platelets without centrifugation. We used 16 polymers of different chemical characteristics and mixed each polymer with fresh whole blood containing anticoagulant citrate-dextrose. Then, we observed blood cell separation. Focusing on the effective polymers and comparing our method with the conventional centrifugation method, we examined platelet recovery rate and Pselectin expression, which represents platelet activation. Poly-L-glutamic acid (PGA) and poly-L-aspartic acid (PAA) separated platelets efficiently; however, these polymers activated platelets. On the contrary, poly (2-methacryloyloxyethyl phosphorylcholine-co-n-butyl methacrylate) (PMB), which has phosphorylcholine residues mimicking a cellular membrane molecule, separated platelets moderately and the PMB-separated platelets were nearly inactive compared to the separation with the ordinary centrifugation and PGA. Conclusively, the present experiments demonstrated that without centrifugation platelets can be separated from whole blood with some water-soluble polymers, such as PGA, PAA, and PMB, and that PMB has an advantage not to activate platelets.

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Interaction of basic polyelectrolytes with the red blood cell II. Agglutination of red blood cells by polymeric bases

Cited by 197 publications

References 16 publications

Intracellular fate of bioresponsive poly(amidoamine)s in vitro and in vivo

Intracellular fate of bioresponsive poly(amidoamine)s in vitro and in vivo

Studies on the Mechanism of Inhibition of Sodium Transport in Cystic Fibrosis of the Pancreas

Platelet Separation From Whole Blood in an Aqueous Two-Phase System With Water-Soluble Polymers

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